LOCUS pLenti6-hKPNA1-V5-blast 8710 bp DNA circular UNA 9-SEP-2009 DEFINITION LMBP 7928. COMMENT This file is created by Vector NTI http://www.invitrogen.com/ FEATURES Location/Qualifiers Misc._feature complement(join(8703..8710,1..13)) /vntifkey="21" /label=attB2 CDS 67..108 /invitrogen="1350000" /vntifkey="4" /label="V5 epitope" Primer complement(76..96) /invitrogen="2140000" /vntifkey="27" /label="V5 reverse primer" CDS 118..126 /invitrogen="123000001" /vntifkey="4" /label="3 stops" Promoter 163..472 /invitrogen="2290000" /vntifkey="29" /label="SV40 early promoter" Promoter 526..593 /invitrogen="2340000" /vntifkey="30" /label="EM7 promoter" CDS 594..992 /invitrogen="1050000" /vntifkey="4" /label=Bsd(R) LTR 1078..1131 /invitrogen="1410000" /vntifkey="19" /label=deltaU3 LTR 1079..1312 /invitrogen="1390000" /vntifkey="19" /label="deltaU3/3' LTR" LTR 1132..1312 /invitrogen="1430000" /vntifkey="19" /label="HIV-1 3' LTR" PolyA_signal 1384..1514 /invitrogen="1840000" /vntifkey="25" /label="SV40 pA" Replication_ori 1887..2342 /invitrogen="2520000" /vntifkey="33" /label="f1 origin" Promoter 2374..2472 /invitrogen="2330000" /vntifkey="30" /label="bla promoter" CDS 2473..3333 /invitrogen="1020000" /vntifkey="4" /label=Amp(R) Replication_ori 3478..4151 /invitrogen="2540000" /vntifkey="33" /label="pUC origin" Promoter 4559..4968 /invitrogen="2280000" /vntifkey="29" /label="RSV/5' LTR hybrid promoter" Promoter 4559..4787 /invitrogen="2270000" /vntifkey="29" /label="RSV promoter" LTR 4788..4968 /invitrogen="1430000" /vntifkey="19" /label="HIV-1 5' LTR" splicing_signal 5078 /invitrogen="123000012" /vntifkey="38" /label="splice donor" Misc._binding 5079..5123 /invitrogen="1780000" /vntifkey="20" /label="HIV-1 psi packaging signal" Protein_binding 5633..5866 /invitrogen="2440000" /vntifkey="31" /label="HIV-1 Rev response element" splicing_signal 6214 /invitrogen="123000013" /vntifkey="38" /label="splice acceptor" splicing_signal 6242 /invitrogen="123000014" /vntifkey="38" /label="splice acceptor" Promoter 6367..6949 /invitrogen="2170000" /vntifkey="29" /label="CMV promoter" Primer 6832..6852 /invitrogen="1930000" /vntifkey="27" /label="CMV forward primer" TATA_box 6867..6870 /invitrogen="123000002" /vntifkey="41" /label="TATA box" Misc._feature 6999..7019 /vntifkey="21" /label=attB1 Misc._feature 7070..8683 /vntifkey="21" /label=KPNA1-S /note="sequence BC090864" ORIGIN 1 TTGTACAAAG TGGTTGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGCGG 61 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTTAG 121 TAATGAGTTT GGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 181 GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT 241 GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA 301 GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC 361 CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT 421 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 481 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT GACAATTAAT 541 CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA ACCATGGCCA 601 AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA ATCAACAGCA 661 TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC GGCCGCATCT 721 TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC GTGGTGCTGG 781 GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC GGAAATGAGA 841 ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT CTGCATCCTG 901 GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG ATTCGTGAAT 961 TGCTGCCCTC TGGTTATGTG TGGGAGGGCT AAGCACAATT CGAGCTCGGT ACCTTTAAGA 1021 CCAATGACTT ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG 1081 GAAGGGCTAA TTCACTCCCA ACGAAGACAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC 1141 TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG 1201 CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT 1261 GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT 1321 TCATGTCATC TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG 1381 AGGAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC 1441 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA 1501 TCTTATCATG TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 1561 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 1621 CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 1681 TAGGGACGTA CCCAATTCGC CCTATAGTGA GTCGTATTAC GCGCGCTCAC TGGCCGTCGT 1741 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 1801 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 1861 GTTGCGCAGC CTGAATGGCG AATGGGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 1921 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 1981 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 2041 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 2101 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 2161 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 2221 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 2281 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT 2341 TTAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 2401 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 2461 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 2521 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 2581 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 2641 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 2701 GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 2761 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 2821 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 2881 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 2941 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 3001 GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 3061 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 3121 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 3181 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC 3241 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 3301 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 3361 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 3421 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 3481 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 3541 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 3601 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG 3661 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 3721 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 3781 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 3841 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 3901 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 3961 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 4021 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 4081 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 4141 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 4201 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 4261 GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT 4321 TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT 4381 AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT 4441 ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT 4501 TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA 4561 TGTAGTCTTA TGCAATACTC TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG 4621 CCTTACAAGG AGAGAAAAAG CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC 4681 GTGCCTTATT AGGAAGGCAA CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC 4741 CGCATTGCAG AGATATTGTA TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT 4801 TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC 4861 AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA 4921 ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA 4981 CAGGGACTTG AAAGCGAAAG GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC 5041 TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC 5101 TAGCGGAGGC TAGAAGGAGA GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT 5161 TAGATCGCGA TGGGAAAAAA TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA 5221 ACATATAGTA TGGGCAAGCA GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA 5281 AACATCAGAA GGCTGTAGAC AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC 5341 AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT 5401 AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA 5461 GACCACCGCA CAGCAAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA 5521 ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC 5581 CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT 5641 TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA 5701 CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG 5761 CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG 5821 CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT 5881 GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT 5941 CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT 6001 ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC 6061 AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT 6121 GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG 6181 TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC 6241 AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG 6301 GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGATAAG 6361 CTTGGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG 6421 ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT 6481 TCCATTGACG TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG 6541 TGTATCATAT GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC 6601 ATTATGCCCA GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 6661 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT 6721 TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC 6781 ACCAAAATCA ACGGGACTTT CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG 6841 GCGGTAGGCG TGTACGGTGG GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA 6901 TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTAGAGGA 6961 TCCACTAGTC CAGTGTGGTG GAATTCTGCA GATATCAACA AGTTTGTACA AAAAAGCAGG 7021 CTCTTTAAAG GAACCAATTC AGTCGACTGG ATCCGGTACC GAATTCACCA TGACCACCCC 7081 AGGAAAAGAG AACTTTCGCC TGAAAAGTTA CAAGAACAAA TCTCTGAATC CCGATGAGAT 7141 GCGCAGGAGG AGGGAGGAAG AAGGACTGCA GTTACGAAAG CAGAAAAGAG AAGAGCAGTT 7201 ATTCAAGCGG AGAAATGTTG CTACAGCAGA AGAAGAAACA GAAGAAGAAG TTATGTCAGA 7261 TGGAGGCTTT CATGAGGCTC AGATTAATAA CATGGAGATG GCACCAGGTG GTGTCATCAC 7321 TTCTGACATG ATTGAAATGA TATTTTCCAA AAGCCCAGAG CAACAGCTTT CAGCAACACA 7381 GAAATTCAGG AAGCTGCTTT CAAAAGAACC TAACCCTCCT ATTGATGAAG TTATCAGCAC 7441 ACCAGGAGTA GTGGCCAGGT TTGTGGAGTT CCTCAAACGA AAAGAGAATT GTACACTGCA 7501 GTTTGAATCA GCTTGGGTAC TGACAAATAT TGCTTCAGGA AATTCTCTTC AGACCCGAAT 7561 TGTGATTCAG GCAGGAGCTG TGCCCATCTT CATAGAGTTG CTCAGCTCAG AGTTTGAAGA 7621 TGTCCAGGAA CAGGCAGTCT GGGCTCTTGG CAACATTGCT GGAGATAGTA CCATGTACAG 7681 GGACTATGTC TTAGACTGCA ATATCCTTCC CCCTCTTTTG CAGTTATTTT CAAAGCAAAA 7741 CCGCCTGACC ATGACCCGGA ATGCAGTATG GGCTTTGTCT AATCTCTGTA GAGGGAAAAG 7801 TCCACCTCCA GAATTTGCAA AGGTTTCTCC ATGTCTGAAT GTGCTTTCCT GGTTGCTGTT 7861 TGTCAGTGAC ACTGATGTAC TGGCTGATGC CTGCTGGGCC CTCTCATATC TATCAGATGG 7921 ACCCAATGAT AAAATTCAAG CGGTCATCGA TGCGGGAGTA TGTAGGAGAC TTGTGGAACT 7981 GCTGATGCAT AATGATTATA AAGTGGTTTC TCCTGCTTTG CGAGCTGTGG GAAACATTGT 8041 CACAGGGGAT GATATTCAGA CACAGGTAAT TCTGAATTGC TCAGCTCTGC AGAGTTTATT 8101 GCATTTGCTG AGTAGCCCAA AGGAATCTAT CAAAAAGGAA GCATGTTGGA CGATATCTAA 8161 TATTACAGCT GGAAATAGGG CACAGATCCA GACTGTGATA GATGCCAACA TTTTCCCAGC 8221 CCTCATTAGT ATTTTACAAA CTGCTGAATT TCGGACAAGA AAAGAAGCAG CTTGGGCCAT 8281 CACAAATGCA ACTTCTGGAG GATCAGCTGA ACAGATCAAG TACCTAGTAG AACTGGGTTG 8341 TATCAAGCCG CTCTGTGATC TCCTCACGGT CATGGACTCT AAGATTGTAC AGGTTGCCCT 8401 AAATGGCTTG GAAAATATCC TGAGGCTTGG AGAACAGGAA GCCAAAAGGA ATGGCACTGG 8461 CATTAACCCT TACTGTGCTT TGATTGAAGA AGCTTATGGT CTGGATAAAA TTGAGTTCTT 8521 ACAGAGTCAT GAAAACCAGG AGATCTACCA AAAGGCCTTT GATCTTATTG AGCATTACTT 8581 CGGGACCGAA GATGAAGACA GCAGCATTGC ACCCCAGGTT GACCTTAACC AGCAGCAGTA 8641 CATCTTCCAA CAGTGTGAGG CTCCTATGGA AGGTTTCCAG CTTCACTCGA GATATCTAGA 8701 CCCAGCTTTC //