LOCUS       pMET7-mLR               8065 bp    DNA     circular     31-OCT-2012
DEFINITION  LMBP 7840.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 7840
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 31-OCT-2012
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|641319841|
COMMENT     VNTDBDATE|641398090|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMET7-mLR|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT            0 4253 0 5796 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 1464 0 6114 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 5889 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 7627 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 822 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "9..632") 
COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 0 247 322 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "247..322") 
COMMENT        #7=(CFSignal (CObList) "T7 promoter" 30 0 0 800 816 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "800..816") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5948 6044 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "5948..6044") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5948 6044 0 
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COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 1 7073 7933 0 
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COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 6130 6532 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pMET7-mLR'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 10/30/12 04:24" 1) 
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FEATURES             Location/Qualifiers
     promoter        9..632
                     /vntifkey="29"
                     /label=SRalpha\promoter
                     /note="SV40 early/human T-cell lymphotropic virus LTR hybrid promoter"
     rep_origin      247..322
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        800..816
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     terminator      5948..6044
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5948..6044)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(7073..7933)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             866..3550
                     /vntifkey="4"
                     /label=mLepr
     misc_feature    3551..5884
                     /vntifkey="21"
                     /label=3'\UTR\of\mLepr
     rep_origin      complement(6130..6532)
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     2274 a      1761 c      1735 g      2295 t 
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat 
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact 
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag 
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc 
      781 tctaaaagct gcggaattct aatacgactc actataggga gtcgactcag atcttcgcaa 
      841 tccaggtgta cacctctgaa gaaagatgat gtgtcagaaa ttctatgtgg ttttgttaca 
      901 ctgggaattt ctttatgtga tagctgcact taacctggca tatccaatct ctccctggaa 
      961 atttaagttg ttttgtggac caccgaacac aaccgatgac tcctttctct cacctgctgg 
     1021 agccccaaac aatgcctcgg ctttgaaggg ggcttctgaa gcaattgttg aagctaaatt 
     1081 taattcaagt ggtatctacg ttcctgagtt atccaaaaca gtcttccact gttgctttgg 
     1141 gaatgagcaa ggtcaaaact gctctgcact cacagacaac actgaaggga agacactggc 
     1201 ttcagtagtg aaggcttcag tttttcgcca gctaggtgta aactgggaca tagagtgctg 
     1261 gatgaaaggg gacttgacat tattcatctg tcatatggag ccattaccta agaacccctt 
     1321 caagaattat gactctaagg tccatctttt atatgatctg cctgaagtca tagatgattc 
     1381 gcctctgccc ccactgaaag acagctttca gactgtccaa tgcaactgca gtcttcgggg 
     1441 atgtgaatgt catgtgccgg tacccagagc caaactcaac tacgctcttc tgatgtattt 
     1501 ggaaatcaca tctgccggtg tgagttttca gtcacctctg atgtcactgc agcccatgct 
     1561 tgttgtgaaa cccgatccac ccttaggttt gcatatggaa gtcacagatg atggtaattt 
     1621 aaagatttct tgggacagcc aaacaatggc accatttccg cttcaatatc aggtgaaata 
     1681 tttagagaat tctacaattg taagagaggc tgctgaaatt gtctcagcta catctctgct 
     1741 ggtagacagt gtgcttcctg gatcttcata tgaggtccag gtgaggagca agagactgga 
     1801 tggttcagga gtctggagtg actggagttc acctcaagtc tttaccacac aagatgttgt 
     1861 gtattttcca cccaaaattc tgactagtgt tggatcgaat gcttcttttc attgcatcta 
     1921 caaaaacgaa aaccagatta tctcctcaaa acagatagtt tggtggagga atctagctga 
     1981 gaaaatccct gagatacagt acagcattgt gagtgaccga gttagcaaag ttaccttctc 
     2041 caacctgaaa gccaccagac ctcgagggaa gtttacctat gacgcagtgt actgctgcaa 
     2101 tgagcaggcg tgccatcacc gctatgctga attatacgtg atcgatgtca atatcaatat 
     2161 atcatgtgaa actgacgggt acttaactaa aatgacttgc agatggtcac ccagcacaat 
     2221 ccaatcacta gtgggaagca ctgtgcagct gaggtatcac aggcgcagcc tgtattgtcc 
     2281 tgatagtcca tctattcatc ctacgtctga gcccaaaaac tgcgtcttac agagagacgg 
     2341 cttttatgaa tgtgttttcc agccaatctt tctattatct ggctatacaa tgtggatcag 
     2401 gatcaaccat tctttaggtt cacttgactc gccaccaacg tgtgtccttc ctgactccgt 
     2461 agtaaaacca ctacctccat ctaacgtaaa agcagagatt actgtaaaca ctggattatt 
     2521 gaaagtatct tgggaaaagc cagtctttcc ggagaataac cttcaattcc agattcgata 
     2581 tggcttaagt ggaaaagaaa tacaatggaa gacacatgag gtattcgatg caaagtcaaa 
     2641 gtctgccagc ctgctggtgt cagacctctg tgcagtctat gtggtccagg ttcgctgccg 
     2701 gcggttggat ggactaggat attggagtaa ttggagcagt ccagcctata cgcttgtcat 
     2761 ggatgtaaaa gttcctatga gagggcctga attttggaga aaaatggatg gggacgttac 
     2821 taaaaaggag agaaatgtca ccttgctttg gaagcccctg acgaaaaatg actcactgtg 
     2881 tagtgtgagg aggtacgtgg tgaagcatcg tactgcccac aatgggacgt ggtcagaaga 
     2941 tgtgggaaat cggaccaatc tcactttcct gtggacagaa ccagcgcaca ctgttacagt 
     3001 tctggctgtc aattccctcg gcgcttccct tgtgaatttt aaccttacct tctcatggcc 
     3061 catgagtaaa gtgagtgctg tggagtcact cagtgcttat cccctgagca gcagctgtgt 
     3121 catcctttcc tggacactgt cacctgatga ttatagtctg ttatatctgg ttattgaatg 
     3181 gaagatcctt aatgaagatg atggaatgaa gtggcttaga attccctcga atgttaaaaa 
     3241 gttttatatc cacgataatt ttattcccat cgagaaatat cagtttagtc tttacccagt 
     3301 atttatggaa ggagttggaa aaccaaagat aattaatggt ttcaccaaag atgctatcga 
     3361 caagcagcag aatgacgcag ggctgtatgt cattgtaccc ataattattt cctcttgtgt 
     3421 cctactgctc ggaacactgt taatttcaca ccagagaatg aaaaagttgt tttgggacga 
     3481 tgttccaaac cccaagaatt gttcctgggc acaaggactg aatttccaaa agagaacgga 
     3541 cactctttga agtctctcat gaccactaca gatgaaccca atctaccaac ttcccaacag 
     3601 tccatacaat attagaagat gtttacattt tgatggaggg aaacaaacct aaactatggt 
     3661 ttgaatgact aagaaataac atttgatgag cttattagag aagtgtatat tttgtggcca 
     3721 caatgtaggt ttgatgtagt tcagtttgga catatgcttg attttcaggg catcaaaaat 
     3781 ttaaagttga tattcatgga ctctgcattt tatttcttaa gtcataaaat gataatggtg 
     3841 tgacggttgc tgtcagaacc tattttgtta cagatcacca aatatggtag gtaatgcctt 
     3901 aactaagtga actctaatag gcaatttttg acacacagct atgcaaaaat ccacatacaa 
     3961 actgcaggga aggaattcca ttttcatttc agtgtgtgaa agcttccata ttagattcca 
     4021 ttagggggct ggtgagatgg ctcagtggtt aagagcgccg actgctcttc caaaggtcct 
     4081 gagttcaaat cccagcaacc acatggtggc tcacaaccat ccgtaatgaa atccgatccc 
     4141 atcttctgga gtgtctgaag acagctacag tgtacttaca ttaaataaat agataaatag 
     4201 atagataaat aaataaatat taaaaaagat ttcattatag tcacacaaat tgaattccag 
     4261 cattctcttg aataatgtgg cttttatata ccaaacttgg aacagtttag gaatttcagt 
     4321 tgtcgatagt cttgaaaatg gcttaaatgc taactttcct tttagttcaa agaaaggcag 
     4381 aagttgtggt ttgggctgat cacagtgcag tggaagctat taggataacc ctacaaactc 
     4441 aggtcagaga tcagtgggag ccattagtct aacctacaca ctcaggtcag attgcccaga 
     4501 gcagaaaaga ataccaacct ccatgaatct aattcatttc tactgaatca gccaagactt 
     4561 ctggcagaca gggaagtatg caggggaggg agactttctg gttatacaaa tacttcacag 
     4621 tgaggatagg attttttctc cctgaaacac agatcaattg ccatgtctgt atgatgttaa 
     4681 tatagcatag gctttattgc ttatattcaa actaaaccaa actgatagac acaaataaca 
     4741 ttctcattta agatatgtga gtcacataga ggtgattgat aattttattt ggatgactaa 
     4801 ttttaaaaat taagatacta ggaagactgt atcctttttt cttcttcatt agctattaac 
     4861 agatatcatt ttaatataaa gttcaactgt gaacttgtaa aggttattgt tttcactttt 
     4921 tccaccaata gaaggaaaca aaatttgaac aaattaactt tttagaagtt ttctgaccac 
     4981 tgataagata tagttcagat acatgcagtt ataaaaacaa aacaaaacat tgcttagtga 
     5041 aaccttcctg atgaatctgt ttaagagagc aatgacttat tccataatta atgactcatt 
     5101 aagaaccttt agggatcatg gagtacaaga aagagatcat ttgaggaaga cgcatgagct 
     5161 caactgcaca ctctgtgtgg ttttcatttt tccacattgg tggaatgaag ataacaagtg 
     5221 gatcagatgc tgagctgagt tagggttttg aaccaatttg aagatataga aatagtagaa 
     5281 gcattaaagg acaactaaaa tgtttgaagg aaatcacaaa ggttctgtcg gacacaaaat 
     5341 gggacagaat tttctcattg tatatcattt agtaacacta tttgcccttc agtattttga 
     5401 cacttacata gataggtcta tgccattatc actatattta gtgtttgaca tatttgttct 
     5461 tgtatggttt taatcactgc tatacttcag ttaagagtgt gcaacagctg tgtttttaca 
     5521 tgggatatac ttgccatggt gaagaacatt tgagtgcacc actatctcag gcaataattc 
     5581 aaaaatcctt tcaagcttaa aacaccgtaa cattcaaagg ctaagttacc tatacgatct 
     5641 gtaaattgaa tacattttct atttagttgt gctaactatt caatgtgatg tatccgcagt 
     5701 ggttttcgtt ttgcattttg atgtgtttac attgtttgta ctgagctttt tgcacgtgga 
     5761 tttttttttt ttttgatttg tagctgcttg gcaggaattc attgttatgt aacccttgtt 
     5821 cttatatata aaaaatatat atatatagta caaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     5881 aaaagggcgg ccgctagagt ctctagagaa aaaacctccc acacctcccc ctgaacctga 
     5941 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 
     6001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 
     6061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccccggg taccgagctc 
     6121 gaattaattg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 
     6181 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 
     6241 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 
     6301 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 
     6361 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 
     6421 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 
     6481 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt 
     6541 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 
     6601 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 
     6661 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 
     6721 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 
     6781 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 
     6841 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 
     6901 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 
     6961 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 
     7021 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 
     7081 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 
     7141 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 
     7201 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 
     7261 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 
     7321 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 
     7381 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 
     7441 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 
     7501 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 
     7561 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 
     7621 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 
     7681 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 
     7741 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 
     7801 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 
     7861 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 
     7921 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 
     7981 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 
     8041 catttccccg aaaagtgcca cctgc 
//
