ID pB2H-delta-omega standard; circular DNA; ; 8342 BP. DT 22-JUN-2010 DE LMBP 6701. CC Accession number: LMBP 6701 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 12-JUL-2010 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT rep_origin 5446..5848 FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(6094..6285) FT /vntifkey="4" FT /label=rop FT promoter 8326..8342 FT /vntifkey="30" FT /label=T7\promoter FT promoter complement(2539..2573) FT /vntifkey="30" FT /label=tac\promoter FT promoter complement(2603..2619) FT /vntifkey="30" FT /label=SP6\promoter FT promoter complement(2680..2763) FT /vntifkey="30" FT /label=lac\promoter FT terminator 3388..3435 FT /vntifkey="43" FT /label=T7\terminator FT rep_origin 3472..3927 FT /vntifkey="33" FT /label=f1\ori FT CDS complement(4042..4902) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(78..2441) FT /vntifkey="4" FT /label=lacZ\delta-omega\fragment FT promoter complement(7940..8017) FT /vntifkey="30" FT /label=lacI\promoter FT /note="repressor lacI promoter, incl. RBS" FT CDS complement(6857..7939) FT /vntifkey="4" FT /label=lacI FT RBS complement(2503..2538) FT /vntifkey="32" FT /label=5'\UTR\of\lacZ\gene,\incl.\RBS SQ Sequence 8342 BP; 1903 A; 2253 C; 2198 G; 1988 t; ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag 60 gagatatacc atggttatgc acgggtgaac tgatcgcgca gcggcgtcag cagttgtttt 120 ttatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg ccaacgctta 180 ttacccagct cgatgcaaaa atccatttcg ctggtggtca gatgcgggat ggcgtgggac 240 gcggcgggga gcgtcacact gaggttttcc gccagacgcc actgctgcca ggcgctgatg 300 tgcccggctt ctgaccatgc ggtcgcgttc ggttgcacta cgcgtactgt gagccagagt 360 tgcccggcgc tctccggctg cggtagttca ggcagttcaa tcaactgttt accttgtgga 420 gcgacatcca gaggcacttc accgcttgcc agcggcttac catccagcgc caccatccag 480 tgcaggagct cgttatcgct atgacggaac aggtattcgc tggtcacttc gatggtttgc 540 ccggataaac ggaactggaa aaactgctgc tggtgttttg cttccgtcag cgctggatgc 600 ggcgtgcggt cggcaaagac cagaccgttc atacagaact ggcgatcgtt cggcgtatcg 660 ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt catcatattt aatcagcgac 720 tgatccaccc agtcccagac gaagccgccc tgtaaacggg gatactgacg aaacgcctgc 780 cagtatttag cgaaaccgcc aagactgtta cccatcgcgt gggcgtattc gcaaaggatc 840 agcgggcgcg tctctccagg tagcgaaagc cattttttga tggaccattt cggcacagcc 900 gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc ggtggccgtg 960 gtgtcggctc cgccgccttc atactgcacc gggcgggaag gatcgacaga tttgatccag 1020 cgatacagcg cgtcgtgatt agcgccgtgg cctgattcat tccccagcga ccagatgatc 1080 acactcgggt gattacgatc gcgctgcacc attcgcgtta cgcgttcgct catcgccggt 1140 agccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt ttcaatattg 1200 gcttcatcca ccacatacag gccgtagcgg tcgcacagcg tgtaccacag cggatggttc 1260 ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcatcagcag gatatcctgc 1320 accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg acggttaacg 1380 cctcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat ccgcacctcg 1440 cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt gtgcagttca 1500 accaccgcac gatagagatt cgggatttcg gcgctccaca gtttcgggtt ttcgacgttc 1560 agacgtagtg tgacgcgatc ggcataacca ccacgctcat cgataatttc accgccgaaa 1620 ggcgcggtgc cgctggcgac ctgcgtttca ccctgccata aagaaactgt tacccgtagg 1680 tagtcacgca actcgccgca catctgaact tcagcctcca gtacagcgcg gctgaaatca 1740 tcattaaagc gagtggcaac atggaaatcg ctgatttgtg tagtcggttt atgcagcaac 1800 gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag ataactgccg 1860 tcactccaac gcagcaccat caccgcgagg cggttttctc cggcgcgtaa aaatgcgctc 1920 aggtcaaatt cagacggcaa acgactgtcc tggccgtaac cgacccagcg cccgttgcac 1980 cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc ttcctgtagc 2040 cagctttcat caacattaaa tgtgagcgag taacaacccg tcggattctc cgtgggaaca 2100 aacggcggat tgaccgtaat gggataggtt acgttggtgt agatgggcgc atcgtaaccg 2160 tgcatctgcc agtttgaggg gacgacgaca gtatcggcct caggaagatc gcactccagc 2220 cagctttccg gcaccgcttc tggtgccgga aaccaggcaa agcgccattc gccattcagg 2280 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg 2340 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga 2400 cgttgtaaaa cgacggccag tgaatccgta atcatggtca tacccccgga tccgaattcg 2460 agctcggcgc gcctgcagcg gccgcatgca tatggatcga tcctgtttcc tgtgtgaaat 2520 tgttatccgc tcacaattcc acacattata cgagccgatg attaattgtc aacaggggga 2580 tggggagtaa gcttgagtat tctatagtgt cacctaaata gcttggcgta atcatggtca 2640 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 2700 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 2760 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 2820 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 2880 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 2940 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgaattaatt ctcatgtttg 3000 acagcttatc atcggataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt 3060 ctcatgtttg acagcttatc ttcgattagc tttaatgcgg tagtttatca cagttaaatt 3120 gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc atcctcggca 3180 ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg ggcctcttgc 3240 gggatatcgg ccggccacgc gatcgctgac gtcggtaccc tcgagtctgg taaagaaacc 3300 gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagcgcagc ttaattaacc 3360 taggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc 3420 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct 3480 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3540 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3600 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3660 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 3720 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3780 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 3840 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3900 ttaacaaaat attaacgttt acaatttctg gcggcacgat ggcatgagat tatcaaaaag 3960 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 4020 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4080 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4140 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4200 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4260 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4320 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 4380 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4440 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4500 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4560 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4620 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 4680 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 4740 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 4800 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 4860 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaatc 4920 atgattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4980 agaaaaataa acaaataggt catgaccaaa atcccttaac gtgagttttc gttccactga 5040 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 5100 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 5160 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 5220 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 5280 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 5340 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 5400 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 5460 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 5520 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 5580 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 5640 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 5700 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 5760 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 5820 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 5880 tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat 5940 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 6000 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 6060 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 6120 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc 6180 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct 6240 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta 6300 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat 6360 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc 6420 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa 6480 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat 6540 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac 6600 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc 6660 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct 6720 caacgacagg agcacgatca tgctagtcat gccccgcgcc caccggaagg agctgactgg 6780 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac 6840 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 6900 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt 6960 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga 7020 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg 7080 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt 7140 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat 7200 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt 7260 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc 7320 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc 7380 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg 7440 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa 7500 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg 7560 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac 7620 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg 7680 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg 7740 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt 7800 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg 7860 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt 7920 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg 7980 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta 8040 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc 8100 gcaaggaatg gtgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 8160 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 8220 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 8280 tgcgtccggc gtagaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta 8340 ta 8342 //