LOCUS       pMSCV-His-AU-Ub-K11        6586 bp    DNA     circular     16-DEC-2009
DEFINITION  LMBP 6592.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|537807586|
COMMENT     VNTDBDATE|537807586|
COMMENT     LSOWNER|
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COMMENT     VNTAUTHORNAME|Demo User|
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FEATURES             Location/Qualifiers
     misc_feature    4792..5652
                     /vntifkey="21"
                     /label=Amp\(r)
     misc_feature    1424..1450
                     /vntifkey="21"
                     /label=His-tag
     misc_feature    1451..1480
                     /vntifkey="21"
                     /label=AU1-tag
     CDS             1481..1710
                     /vntifkey="4"
                     /label=hUb-
     misc_feature    1496..1498
                     /vntifkey="21"
                     /label=R6
     misc_feature    1511..1513
                     /vntifkey="21"
                     /label=K11
     misc_feature    1559..1561
                     /vntifkey="21"
                     /label=R27
     misc_feature    1565..1567
                     /vntifkey="21"
                     /label=R29
     misc_feature    1577..1579
                     /vntifkey="21"
                     /label=R33
     misc_feature    1664..1666
                     /vntifkey="21"
                     /label=R63
BASE COUNT     1488 a      1880 c      1749 g      1469 t 
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 
     1381 ccctcactcc ttctctaggc gccggaatta gatcaagctt aaaatggtac atcaccatca 
     1441 ccatcaccat gccatggaca cataccgcta catcggatcc atgcagatct tcgtgaggac 
     1501 cctgaccggc aagaccatca ctctggaggt ggagcccagt gacaccatcg aaaatgtgag 
     1561 ggccagaatc caagataggg aaggcatccc ccccgaccag cagaggctca tctttgcagg 
     1621 cggcagctgg aagatggccg cactctttct gactacaaca tccagaggga gtcgaccctg 
     1681 cacctggtcc tgcgtctcag aggtgggtag ggcccaattc taccgggtag gggaggcgct 
     1741 tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac 
     1801 aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt 
     1861 ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc 
     1921 cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt 
     1981 gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata 
     2041 gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg 
     2101 gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt 
     2161 ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct 
     2221 ttcgacctgc agcccaagct agcttaccat gaccgagtac aagcccacgg tgcgcctcgc 
     2281 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     2341 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     2401 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     2461 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     2521 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     2581 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     2641 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     2701 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     2761 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 
     2821 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga 
     2881 aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc tccagaaaaa 
     2941 ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg 
     3001 caaggcatgg aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag 
     3061 agacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 
     3121 ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc 
     3181 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca 
     3241 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc 
     3301 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt 
     3361 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt 
     3421 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa 
     3481 gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga ctcaattagc 
     3541 cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg acagcgcaga 
     3601 agagctgggg agaattaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 
     3661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 
     3721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 
     3781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 
     3841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     3901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
     3961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     4021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     4081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     4141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     4201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     4261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     4321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     4381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     4441 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
     4501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     4561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     4621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     4681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     4741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     4801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     4861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     4921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     4981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     5041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     5101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     5161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     5221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     5281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     5341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     5401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     5461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     5521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     5581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     5641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     5701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     5761 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta 
     5821 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa 
     5881 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 
     5941 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta 
     6001 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 
     6061 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg 
     6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc 
     6181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac 
     6241 ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct 
     6301 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc 
     6361 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg 
     6421 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg 
     6481 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca 
     6541 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaa 
//
