ID pMKSV40/8-4 standard; circular DNA; ; 9529 BP. DT 14-SEP-2007 DE LMBP 652 CC Accession number: LMBP 652 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator complement(436..532) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 436..532 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT CDS complement(533..2413) FT /vntifkey="4" FT /label=2nd\exon\of\SV40\large\T-antigen FT /note="2nd exon of the SV40 large T-antigen" FT intron complement(2414..2759) FT /vntifkey="15" FT /label=intron\of\SV40\large\T-antigen FT /note="intron of the SV40 large T-antigen" FT CDS complement(2760..3005) FT /vntifkey="4" FT /label=1st\exon\of\SV40\large\T-antigen FT /note="1st exon of the SV40 large T-antigen" FT CDS complement(2481..3005) FT /vntifkey="4" FT /label=SV40\small\t-antigen FT /note="SV40 small t-antigen" FT promoter complement(3076..3353) FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT promoter 3109..3404 FT /vntifkey="29" FT /label=SV40\late\promoter FT /note="SV40 late promoter" FT misc_feature 3405..3607 FT /vntifkey="21" FT /label=5'\UTR\of\SV40\late\region FT /note="5' UTR of the SV40 late region" FT intron 3608..4545 FT /vntifkey="15" FT /label=major\intron\16S\mRNA\of\SV40\VP1 FT /note="major intron of the 16S mRNA of the SV40 VP1 gene" FT CDS 4580..5616 FT /vntifkey="4" FT /label=part\of\SV40\VP1 FT /note="part of the SV40 VP1 coding sequence" FT CDS 3643..4701 FT /vntifkey="4" FT /label=SV40\VP2 FT /note="SV40 VP2 coding sequence" FT CDS 7061..7876 FT /vntifkey="4" FT /label=kanamycin FT /note="Tn903 antibiotic resistance gene; coding sequence" FT rep_origin complement(8162..8553) FT /vntifkey="33" FT /label=ColE1\ori FT /note="origin plasmid" FT CDS 9130..9471 FT /vntifkey="4" FT /label=colicin\E1\immunity FT /note="colicin E1 immunity coding sequence" FT misc_feature join(5617..9529,1..377) FT /vntifkey="21" FT /label=pMK16 FT /note="pMK16 part of the plasmid" FT CDS 3997..4701 FT /vntifkey="4" FT /label=SV40\VP3 FT /note="SV40 VP3 coding sequence" FT misc_feature complement(3044..3115) FT /vntifkey="21" FT /label=non-functional\SV40\ori FT /note="non-functional SV40 ori" SQ Sequence 9529 BP; 2567 A; 2180 C; 2090 G; 2692 t; ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360 cacacccgtc ctgtggatcc agacatgata agatacattg atgagtttgg acaaaccaca 420 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt 480 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt 540 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt 600 atggctgatt atgatcatga acagactgtg aggactgagg ggcctgaaat gagccttggg 660 actgtgaatc aatgcctgtt tcatgccctg agtcttccat gttcttctcc ccaccatctt 720 catttttatc agcattttcc tggctgtctt catcatcatc atcactgttt cttagccaat 780 ctaaaactcc aattcccata gccacattaa acttcatttt ttgatacact gacaaactaa 840 actctttgtc caatctctct ttccactcca caattctgct ctgaatactt tgagcaaact 900 cagccacagg tctgtaccaa attaacataa gaagcaaagc aatgccactt tgaattattc 960 tcttttctaa caaaaactca ctgcgttcca ggcaatgctt taaataatct ttgggcctaa 1020 aatctatttg ttttacaaat ctggcctgca gtgttttagg cacactgtac tcattcatgg 1080 tgactattcc agggggaaat atttgagttc ttttatttag gtgtttcttt tctaagttta 1140 ccttaacact gccatccaaa taatccctta aattgtccag gttattaatt ccctgacctg 1200 aaggcaaatc tctggactcc cctccagtgc cctttacatc ctcaaaaact actaaaaact 1260 ggtcaatagc tactcctagc tcaaagttca gcctgtccaa gggcaaatta acatttaaag 1320 ctttcccccc acataattca agcaaagcag ctgctaatgt agttttacca ctatcaattg 1380 gtcctttaaa cagccagtat ctttttttag gaatgttgta caccatgcat tttaaaaagt 1440 catacaccac tgaatccatt ttgggcaaca aacagtgtag ccaagcaact ccagccatcc 1500 attcttctat gtcagcagag cctgtagaac caaacattat atccatccta tccaaaagat 1560 cattaaatct gtttgttaac atttgttctc tagttaattg taggctatca acccgctttt 1620 tagctaaaac agtatcaaca gcctgttggc atatggtttt ttggtttttg ctgtcagcaa 1680 atatagcagc atttgcataa tgcttttcat ggtacttata gtggctgggc tgttcttttt 1740 taatacattt taaacacatt tcaaaactgt actgaaattc caagtacatc ccaagcaata 1800 acaacacatc atcacatttt gtttccattg catactctgt tacaagcttc caggacactt 1860 gtttagtttc ctctgcttct tctggattaa aatcatgctc ctttaaccca cctggcaaac 1920 tttcctcaat aacagaaaat ggatctctag tcaaggcact atacatcaaa tattccttat 1980 taaccccttt acaaattaaa aagctaaagg tacacaattt ttgagcatag ttattaatag 2040 cagacactct atgcctgtgt ggagtaagaa aaaacagtat gttatgatta taactgttat 2100 gcctacttat aaaggttaca gaatattttt ccataatttt cttgtatagc agtgcagctt 2160 tttcctttgt ggtgtaaata gcaaagcaag caagagttct attactaaac acagcatgac 2220 tcaaaaaact tagcaattct gaaggaaagt ccttggggtc ttctaccttt ctcttctttt 2280 ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagat ggcatttctt 2340 ctgagcaaaa caggttttcc tcattaaagg cattccacca ctgctcccat tcatcagttc 2400 cataggttgg aatctaaaat acacaaacaa ttagaatcag tagtttaaca cattatacac 2460 ttaaaaattt tatatttacc ttagagcttt aaatctctgt aggtagtttg tccaattatg 2520 tcacaccaca gaagtaaggt tccttcacaa agatcaagtc caaaccacat tctaaagcaa 2580 tcgaagcagt agcaatcaac ccacacaagt ggatctttcc tgtataattt tctattttca 2640 tgcttcatcc tcagtaagca cagcaagcat atgcagttag cagacatttt ctttgcacac 2700 tcaggccatt gtttgcagta cattgcatca acaccaggat ttaaggaaga agcaaatacc 2760 tcagttgcat cccagaagcc tccaaagtca ggttgatgag catattttac tccatcttcc 2820 attttcttgt acagagtatt cattttcttc attttttctt catctcctcc tttatcagga 2880 tgaaactcct tgcatttttt taaatatgcc tttctcatca gaggaatatt cccccaggca 2940 ctcctttcaa gacctagaag gtccattagc tgcaaagatt cctctctgtt taaaacttta 3000 tccatctttg caaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt 3060 ctggaatagc tcagaggccg ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca 3120 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc 3180 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag 3240 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg 3300 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctggttc 3360 tttccgcctc agaaggtacc taaccaagtt cctctttcag aggttatttc aggccatggt 3420 gctgcgccgg ctgtcacgcc aggcctccgt taaggttcgt aggtcatgga ctgaaagtaa 3480 aaaaacagct caacgccttt ttgtgtttgt tttagagctt ttgctgcaat tttgtgaagg 3540 ggaagatact gttgacggga aacgcaaaaa accagaaagg ttaactgaaa aaccagaaag 3600 ttaactggta agtttagtct ttttgtcttt tatttcaggt ccatgggtgc tgctttaaca 3660 ctgttggggg acctaattgc tactgtgtct gaagctgctg ctgctactgg attttcagta 3720 gctgaaattg ctgctggaga ggccgctgct gcaattgaag tgcaacttgc atctgttgct 3780 actgttgaag gcctaacaac ctctgaggca attgctgcta taggcctcac tccacaggcc 3840 tatgctgtga tatctggggc tcctgctgct atagctggat ttgcagcttt actgcaaact 3900 gtgactggtg tgagcgctgt tgctcaagtg gggtatagat tttttagtga ctgggatcac 3960 aaagtttcta ctgttggttt atatcaacaa ccaggaatgg ctgtagattt gtataggcca 4020 gatgattact atgatatttt atttcctgga gtacaaacct ttgttcacag tgttcagtat 4080 cttgacccca gacattgggg tccaacactt tttaatgcca tttctcaagc tttttggcgt 4140 gtaatacaaa atgacattcc taggctcacc tcacaggagc ttgaaagaag aacccaaaga 4200 tatttaaggg acagtttggc aaggttttta gaggaaacta cttggacagt aattaatgct 4260 cctgttaatt ggtataactc tttacaagat tactactcta ctttgtctcc cattaggcct 4320 acaatggtga gacaagtagc caacagggaa gggttgcaaa tatcatttgg gcacacctat 4380 gataatattg atgaagcaga cagtattcag caagtaactg agaggtggga agctcaaagc 4440 caaagtccta atgtgcagtc aggtgaattt attgaaaaat ttgaggctcc tggtggtgca 4500 aatcaaagaa ctgctcctca gtggatgttg cctttacttc taggcctgta cggaagtgtt 4560 acttctgctc taaaagctta tgaagatggc cccaacaaaa agaaaaggaa gttgtccagg 4620 ggcagctccc aaaaaaccaa aggaaccagt gcaagtgcca aagctcgtca taaaaggagg 4680 aatagaagtt ctaggagtta aaactggagt agacagcttc actgaggtgg agtgcttttt 4740 aaatcctcaa atgggcaatc ctgatgaaca tcaaaaaggc ttaagtaaaa gcttagcagc 4800 tgaaaaacag tttacagatg actctccaga caaagaacaa ctgccttgct acagtgtggc 4860 tagaattcct ttgcctaatt taaatgagga cttaacctgt ggaaatattt tgatgtggga 4920 agctgttact gttaaaactg aggttattgg ggtaactgct atgttaaact tgcattcagg 4980 gacacaaaaa actcatgaaa atggtgctgg aaaacccatt caagggtcaa attttcattt 5040 ttttgctgtt ggtggggaac ctttggagct gcagggtgtg ttagcaaact acaggaccaa 5100 atatcctgct caaactgtaa ccccaaaaaa tgctacagtt gacagtcagc agatgaacac 5160 tgaccacaag gctgttttgg ataaggataa tgcttatcca gtggagtgct gggttcctga 5220 tccaagtaaa aatgaaaaca ctagatattt tggaacctac acaggtgggg aaaatgtgcc 5280 tcctgttttg cacattacta acacagcaac cacagtgctt cttgatgagc agggtgttgg 5340 gcccttgtgc aaagctgaca gcttgtatgt ttctgctgtt gacatttgtg ggctgtttac 5400 caacacttct ggaacacagc agtggaaggg acttcccaga tattttaaaa ttacccttag 5460 aaagcggtct gtgaaaaacc cctacccaat ttcctttttg ttaagtgacc taattaacag 5520 gaggacacag agggtggatg ggcagcctat gattggaatg tcctctcaag tagaggaggt 5580 tagggtttat gaggacacag aggagcttcc tggggatcct ctacgccgga cgcatcgtgg 5640 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg 5700 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg 5760 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg 5820 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata 5880 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg 5940 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag 6000 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga 6060 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg 6120 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc gcggcgagca 6180 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt 6240 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc 6300 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc 6360 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg 6420 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt 6480 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 6540 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 6600 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat 6660 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt 6720 attaacgaag cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg 6780 aatcgcccca tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta 6840 ggtggaccag ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg 6900 aagatgcgtg atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt 6960 tgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat 7020 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga 7080 aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa 7140 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc 7200 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga 7260 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt 7320 tatccgtact cctgatgatg catggttact caccactgcg atccccggga aaacagcatt 7380 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt 7440 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt 7500 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga 7560 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc 7620 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga 7680 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca 7740 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct 7800 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct 7860 cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct 7920 gacttgacgg gacggcggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag 7980 atcacgcatc ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc 8040 aactggtcca cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat 8100 gatggggcga ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag 8160 cgcttttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg 8220 gtactgactc acacaaaaac ggtaacacag ttatccacag aatcagggga taaggccgga 8280 aagaacatgt gagcaaaaga ccaggaacag gaagaaggcc acgtagcagg cgtttttcca 8340 taggctccgc ccccctgacg agcatcacaa aaatagacgc tcaagtcaga ggtggcgaaa 8400 cccgacagga ctataaagct accaggcgtt tccccctgga agctccctcg tgcgctctcc 8460 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 8520 gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc gctccaagct 8580 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 8640 tcttgagtcc aacccggtaa ggcacgcctt aacgccactg gcagcagcca ctggtaaccg 8700 gattagcaga gcgatgatgg cacaaacggt gctacagagt tcttgaagta gtggcccgac 8760 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 8820 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgttggtag cggtggtttt 8880 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttaatc 8940 ttttctactg aaccgcgatc cccgtcagtt tagaagagga ggatggtgcg atggtccctc 9000 cctgaacatc aggtatatag ttagcctgac atccaacaag gaggtttatc gcgaatattc 9060 ccacaaaaaa tcttttcctc ataactcgat ccttataaaa tgaaaagaat atatggcgag 9120 gtttaattta tgagcttaag atactacata aaaaatattt tatttggcct gtactgcaca 9180 cttatatata tataccttat aacaaaaaac agcgaagggt attatttcct tgtgtcagat 9240 aagatgctat atgcaatagt gataagcact attctatgtc catattcaaa atatgctatt 9300 gaatacatag cttttaactt cataaagaaa gattttttcg aaagaagaaa aaacctaaat 9360 aacgcccccg tagcaaaatt aaacctattt atgctatata atctactttg tttggtccta 9420 gcaatcccat ttggattgct aggacttttt atatcaataa agaataatta aatccctaac 9480 acctcattta tagtattaag tttattctta tcaatatagg agcatagaa 9529 //