LOCUS pLHX32 3398 bp DNA circular SYN 29-OCT-2014 DEFINITION LMBP 6312. ACCESSION pLHX32 SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 3398) AUTHORS Nico Mertens JOURNAL Notebook COMMENT Accession number: LMBP 6312 (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) Last modified: 29-OCT-2014 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|713094290| COMMENT LSOWNER| COMMENT VNTNAME|pLHX32| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT VNTREPLTYPE|Plasmid COMMENT VNTEXTCHREPL|Bacteria COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 29-OCT-2014" COMMENT "Last modified: 29-OCT-2014" 65535 0 -4.46243 0 -10 COMMENT 11.1809 1.06602 Nil)) (CObjectList)) COMMENT #242=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1381 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 5524992 3539006 (CObList))) FEATURES Location/Qualifiers CDS 131..455 /gene="TRX" /product="TRX" /SECDrawAs="Gene" /vntifkey="4" /label=TRX CDS complement (1926..2786) /gene="bla" /product="bla" /SECDrawAs="Gene" /vntifkey="4" /label=ampicillin\resistance promoter 3152..3282 /vntifkey="30" /label=PL\promoter promoter 24..40 /vntifkey="30" /label=T7\promoter RBS 41..103 /vntifkey="32" /label=5'\UTR\of\T7\gene\10,\incl.\RBS misc_marker 107..124 /vntifkey="22" /label=His-tag CDS 470..484 /vntifkey="4" /label=EK\recognition\site promoter complement(571..587) /vntifkey="30" /label=T3\promoter terminator 726..773 /vntifkey="43" /label=T7\terminator terminator 605..652 /vntifkey="43" /label=T7\terminator terminator 846..927 /vntifkey="43" /label=fd\terminator terminator 956..1037 /vntifkey="43" /label=fd\terminator rep_origin 1107..1385 /vntifkey="33" /label=pMB1\ori BASE COUNT 903 a 829 c 807 g 859 t ORIGIN 1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 61 ctctagaaat aattttgttt aactttaaga aggagatata catatgcatc atcaccatca 121 ccatcccggg agcgataaaa ttattcacct gactgacgac agttttgaca cggatgtact 181 caaagcggac ggggcgatcc tcgtcgattt ctgggcagag tggtgcggtc cgtgcaaaat 241 gatcgccccg attctggatg aaatcgctga cgaatatcag ggcaaactga ccgttgcaaa 301 actgaacatc gatcaaaacc ctggcactgc gccgaaatat ggcatccgtg gtatcccgac 361 tctgctgctg ttcaaaaacg gtgaagtggc ggcaaccaaa gtgggtgcac tgtctaaagg 421 tcagttgaaa gagttcctcg acgctaacct ggccggttct ggttctggtg atgacgatga 481 caaggtacca agcttatgca tgcggccgca tctagagggc ccggatccct cgaggtcgac 541 gaattcgagc tcggccgact tggccttccc tttagtgagg gttaataaac ttggtgagca 601 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg 661 aggaactata tgcgctcata cgatatgaac gttgagactg ccgctgagtt atcagtgagc 721 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag 781 gaggaactat atccggccgg atagcttatc gctagaggtc gaaattcacc tcgaaagcaa 841 gctgataaac cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa 901 cgtgaaaaaa ttattattcg caattccaag ctaattcacc tcgaaagcaa gctgataaac 961 cgatacaatt aaaggctcct tttggagcct ttttttttgg agattttcaa cgtgaaaaaa 1021 ttattattcg caattccaag ctctgcctcg cgcgtttcgg tgatgacggt gaaaacctct 1081 gacacatgca gctccctagg caattgcatg tgagcaaaag gccagcaaaa ggccaggaac 1141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 1201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 2041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 2101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 2161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 2221 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2521 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 2581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag 2821 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 2881 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca 2941 tcttcccgac aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc 3001 cacctacaac aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc 3061 gattcaggcc tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggtg 3121 atgccggcca cgatgcgtcc ggcgtagagg atctctcacc taccaaacaa tgcccccctg 3181 caaaaaataa attcatataa aaaacataca gataaccatc tgcggtgata aattatctct 3241 ggcggtgttg acataaatac cactggcggt gatactgagc acatcagcag gacgcactga 3301 ccaccatgaa ggtgacgctc ttaaaattaa gccctgaaga agggcagcat tcaaagcaga 3361 aggctttggg gtgtgtgata cgaaacgaag cattggaa //