ID pPLc28LIG8 standard; circular DNA; ; 4708 BP. DT 7-SEP-2009 DE LMBP 574. CC Accession number: LMBP 574 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 7-SEP-2009 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS 538..2001 FT /vntifkey="4" FT /label=phage\T4\ligase FT rep_origin complement(2293..2695) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(3236..4096) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 4462..4592 FT /vntifkey="30" FT /label=PL\promoter FT RBS 18..537 FT /vntifkey="32" FT /label=5'\UTR\of\phage\T4\ligase,\incl.\RBS SQ Sequence 4708 BP; 1386 A; 987 C; 1072 G; 1263 t; ttccggatcc ggccaagctt ttttcaccaa aggaaaataa agaaagtttt gatagtgaaa 60 aagctataaa agatgtaatt gaaaagatga ttaaaaacga ttcttttcct tggaacacta 120 cctggagaac tcctggattt aatccttata atccatatca tccatatcag acgcatccgt 180 ttcagacatg gaactatatt aagcccggcg gtatagagta tttgtataat agacctactt 240 gtggtgataa tattttccaa ggagcattct aatgtttgtt gttcacacta tttatgaaaa 300 tgaaggtaat actacacgtg attacggtca cgtaaatcaa ttttttagat gcaatccaga 360 attccgagct caaaaagacg aacgaatttt taaaaaatgt gtagagcaag gtttcattta 420 tatcaagcac tggatgcaag gaaataaagt tagaaccacg taccacaggt ctttgactga 480 gcttaatgat gaattgattt ataatagagc tgtaaaccaa actctaaagg atgaacaatg 540 attcttaaaa ttctgaacga aatagcatct attggttcaa ctaaacagaa gcaagcaatt 600 cttgaaaaga ataaagataa tgaattgctt aaacgagtat atcgtctgac ttattctcgt 660 gggttacagt attatatcaa gaaatggcct aaacctggta ttgctaccca gagttttgga 720 atgttgactc ttaccgatat gcttgacttc attgaattca cattagctac tcggaaattg 780 actggaaatg cagcaattga ggaattaact ggatatatca ccgatggtaa aaaagatgat 840 gttgaagttt tgcgtcgagt gatgatgcga gaccttgaat gtggtgcttc agtatctatt 900 gcaaacaaag tttggccagg tttaattcct gaacaacctc aaatgctcgc aagttcttat 960 gatgaaaaag gcattaataa gaatatcaaa tttccagcct ttgctcagtt aaaagctgat 1020 ggagctcggt gttttgctga agttagaggt gatgaattag atgatgttcg tcttttatca 1080 cgagctggta atgaatatct aggattagat cttcttaagg aagagttaat taaaatgacc 1140 gctgaagccc gccagattca tccagaaggt gtgttgattg atggcgaatt ggtataccat 1200 gagcaagtta aaaaggagcc agaaggccta gattttcttt ttgatgctta tcctgaaaac 1260 agtaaagcta aagaattcgc cgaagtagct gaatcacgta ctgcttctaa tggaatcgcc 1320 aataaatctt taaagggaac catttctgaa aaagaagcac aatgcatgaa gtttcaggtc 1380 tgggattatg tcccgttggt agaaatatac agtcttcctg catttcgttt gaaatatgat 1440 gtacgttttt ctaaactaga acaaatgaca tctggatatg ataaagtaat tttaattgaa 1500 aaccaggtag taaataacct agatgaagct aaggtaattt ataaaaagta tattgaccaa 1560 ggtcttgaag gtattattct caaaaatatc gatggattat gggaaaatgc tcgttcaaaa 1620 aatctttata aatttaaaga agtaattgat gttgatttaa aaattgtagg aatttatcct 1680 caccgtaaag accctactaa agcgggtgga tttattcttg agtcagagtg tggaaaaatt 1740 aaggtaaatg ctggttcagg cttaaaagat aaagccggtg taaaatcgca tgaacttgac 1800 cgtactcgca ttatggaaaa ccaaaattat tatattggaa aaattctaga gtgcgaatgc 1860 aacggttggt taaaatctga tggccgcact gattacgtta aattatttct tccgattgcg 1920 attcgtttac gtgaagataa aactaaagct aatacattcg aagatgtatt tggtgatttt 1980 catgaggtaa ctggtctatg aaagcttggc tgcctcgcgc gtttcggtga tgacggtgaa 2040 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 2100 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 2160 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 2220 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 2280 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 2340 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 2400 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 2460 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 2520 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 2580 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 2640 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 2700 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 2760 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 2820 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 2880 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 2940 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3000 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3060 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3120 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3180 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 3240 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 3300 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 3360 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 3420 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 3480 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 3540 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 3600 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 3660 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 3720 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3780 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3840 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3900 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 3960 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4020 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4080 aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag ttttattgtt 4140 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg 4200 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac 4260 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac 4320 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc 4380 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggtg atgccggcca 4440 cgatgcgtcc ggcgtagagg atctctcacc taccaaacaa tgcccccctg caaaaaataa 4500 attcatataa aaaacataca gataaccatc tgcggtgata aattatctct ggcggtgttg 4560 acataaatac cactggcggt gatactgagc acatcagcag gacgcactga ccaccatgaa 4620 ggtgacgctc ttaaaattaa gccctgaaga agggcagcat tcaaagcaga aggctttggg 4680 gtgtgtgata cgaaacgaag cattggaa 4708 //