ID pMSCV-F-BirA standard; circular DNA; ; 7370 BP. DT 12-FEB-2009 DE LMBP 5689. CC Accession number: LMBP 5689 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 12-FEB-2009 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT misc_feature 516..1404 FT /vntifkey="21" FT /label=psi+\extended\packaging\signal FT promoter 1..513 FT /vntifkey="29" FT /label=5'\MSCV-PCMV\LTR FT CDS 1479..2444 FT /vntifkey="4" FT /label=birA FT /note="E. coli biotin protein ligase" FT misc_marker 1443..1466 FT /vntifkey="22" FT /label=FLAG\epitope FT terminator 3733..4245 FT /vntifkey="43" FT /label=3'\MSCV-PCMV\LTR FT promoter 2506..3013 FT /vntifkey="29" FT /label=mPGK1\promoter FT CDS 3033..3632 FT /vntifkey="4" FT /label=puromycin\resistance FT CDS complement(5576..6436) FT /vntifkey="4" FT /label=ampicillin\resistance FT rep_origin complement(4633..5035) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(4458..4541) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7370 BP; 1687 A; 2011 C; 1986 G; 1686 t; tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat 60 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca 120 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga 180 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt 240 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc 300 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc 360 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata 420 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca 480 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga 540 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg 600 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt 660 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa 720 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga 780 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag 840 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa 900 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg 960 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct 1020 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga 1080 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag 1140 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc 1200 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc 1260 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg 1320 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag 1380 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaaactt aagcttacca 1440 tggattacaa ggacgatgac gataaggtac ccggatccat gaaggataac accgtgccac 1500 tgaaattgat tgccctgtta gcgaacggtg aatttcactc tggcgagcag ttgggtgaaa 1560 cgctgggaat gagccgggcg gctattaata aacacattca gacactgcgt gactggggcg 1620 ttgatgtctt taccgttccg ggtaaaggat acagcctgcc tgagcctatc cagttactta 1680 atgctaaaca gatattgggt cagctggatg gcggtagtgt agccgtgctg ccagtgattg 1740 actccacgaa tcagtacctt cttgatcgta tcggagagct taaatcgggc gatgcttgca 1800 ttgcagaata ccagcaggct ggccgtggtc gccggggtcg gaaatggttt tcgccttttg 1860 gcgcaaactt atatttgtcg atgttctggc gtctggaaca aggcccggcg gcggcgattg 1920 gtttaagtct ggttatcggt atcgtgatgg cggaagtatt acgcaagctg ggtgcagata 1980 aagttcgtgt taaatggcct aatgacctct atctgcagga tcgcaagctg gcaggcattc 2040 tggtggagct gactggcaaa actggcgatg cggcgcaaat agtcattgga gccgggatca 2100 acatggcaat gcgccgtgtt gaagagagtg tcgttaatca ggggtggatc acgctgcagg 2160 aagcggggat caatctcgat cgtaatacgt tggcggccat gctaatacgt gaattacgtg 2220 ctgcgttgga actcttcgaa caagaaggat tggcacctta tctgtcgcgc tgggaaaagc 2280 tggataattt tattaatcgc ccagtgaaac ttatcattgg tgataaagaa atatttggca 2340 tttcacgcgg aatagacaaa cagggggctt tattacttga gcaggatgga ataataaaac 2400 cctggatggg cggtgaaata tccctgcgta gtgcagaaaa ataagaattc tgcagatatc 2460 cagcacagtg gcggccgctc gagtctagag ggcccgttta acgaattcta ccgggtaggg 2520 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 2580 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 2640 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 2700 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 2760 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 2820 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 2880 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 2940 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 3000 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc 3060 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 3120 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 3180 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 3240 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 3300 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 3360 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 3420 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 3480 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 3540 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 3600 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga 3660 ccgaaaggag cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga 3720 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat 3780 tttgcaaggc atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac 3840 agagagacag cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc 3900 tcagggccaa gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac 3960 catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta 4020 accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag 4080 agcccacaac ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc 4140 gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga 4200 gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct 4260 tgaagttgga gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat 4320 tagccactgt tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg 4380 cagaagagct ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt 4440 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 4500 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4560 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4620 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4680 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4740 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4800 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4860 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4920 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4980 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5040 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5100 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5160 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5220 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5280 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5340 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5400 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5460 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5520 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5580 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5640 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5700 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5760 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5820 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5880 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5940 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6000 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6060 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6120 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6180 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6240 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6300 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6360 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6420 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 6480 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6540 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 6600 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 6660 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 6720 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 6780 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 6840 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 6900 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat 6960 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa 7020 cgacggcgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg 7080 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc 7140 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat 7200 gccggccacg atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc 7260 agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga 7320 gccatagata aaataaaaga ttttatttag tctccagaaa aaggggggaa 7370 //