ID pKNG101 standard; circular DNA; ; 6986 BP. DT 29-OCT-2007 DE LMBP 5246. CC Accession number: LMBP 5246 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium). CC Last modified: 8-NOV-2012 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS 696..2335 FT /vntifkey="4" FT /label=streptomycin\resistance FT rep_origin 2373..2760 FT /vntifkey="33" FT /label=R6K\ori FT /note="origin of replication of plasmid R6K (defective pir gene)" FT rep_origin complement(3477..3587) FT /vntifkey="33" FT /label=oriT FT /note="RK2 conjugational DNA transfer origin" FT CDS 4001..4504 FT /vntifkey="4" FT /label=insB FT /note="E. coli insertion sequence IS1 insB" FT CDS complement(4642..6063) FT /vntifkey="4" FT /label=sacB FT /note="B. subtilis levansucrase coding sequence" SQ Sequence 6986 BP; 1641 A; 1677 C; 1765 G; 1903 t; gtcgacggat ccccgggtac cataagtaga agcagcaacc caagtagctt taccagcatc 60 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc 120 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct 180 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc 240 cgggcccgcg gatgcatatg atcttaaggc ctaggtctag agtctttgtt ttgacgccat 300 tagcgtacgt aacaatcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt 360 agacggagta tactagtata gtctatagtc cgtggaatta ttatatttat ctccgacgat 420 attctcatca gtgaaatcca ggggaattct catgtttgac agcttatcat cgataagctt 480 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct 540 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg 600 gttatgccgg tactgccggg cctcttgcgg gataactgca cattcgggat atttctctat 660 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg 720 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc 780 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg 840 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca 900 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac 960 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac 1020 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa 1080 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg 1140 acgaggacaa gagtacgccg ctgcacgatc ttttggctcg tgtcgaacga gagctaccgg 1200 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga 1260 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg 1320 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg 1380 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag 1440 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat 1500 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga 1560 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa 1620 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg 1680 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga 1740 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac 1800 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc 1860 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca 1920 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag 1980 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc 2040 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc 2100 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat 2160 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga 2220 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca 2280 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc 2340 tagatccggc ccgatgcgtc cggcgtagag gatctgaaga tcagcagttc aacctgttga 2400 tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac gtactaagct 2460 ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg gctaacgtac 2520 taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt aatataaatc 2580 agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa tatataaggc 2640 ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatgta acgcactgag 2700 aagcccttag agcctctcaa agcaattttc agtgacacag gaacacttaa cggctgacat 2760 gggaattcta ctgtttgggt gtatgagcta tttgcaaagg aaattggtga tgacaaagct 2820 cggcgctatt tgaagaaaat cgactatggc aacgccgatc ctttttgtcc ggtgttgggt 2880 tgaaggtgaa gccggtcggg gccgcagcgg gggccggctt ttcagccttg cccccctgct 2940 tcggccgccg tggctccggc gtcttgggtg ccggcgcggg ttccgcagcc ttggcctgcg 3000 gtgcgggcac atcggcgggc ttggccttga tgtgccgcct ggcgtgcgag cggaacgtct 3060 cgtaggagaa cttgaccttc cccgtttccc gcatgtgctc ccaaatggtg acgagcgcat 3120 agccggacgc taacgccgcc tcgacatccg ccctcaccgc caggaacgca accgcagcct 3180 catcacgccg gcgcttcttg gccgcgcggg attcaaccca ctcggccagc tcgtcggtgt 3240 agctctttgg catcgtctct cgcctgtccc ctcagttcag taatttcctg catttgcctg 3300 tttccagtcg gtagatattc cacaaaacag cagggaagca gcgcttttcc gctgcataac 3360 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 3420 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 3480 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 3540 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg 3600 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac 3660 ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg 3720 ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg 3780 tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg 3840 gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca 3900 cgatccgggc aacgttgttg ccattgctgc agttcactta caccgcttct caacccggta 3960 cgcaccataa aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca 4020 ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt 4080 aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg 4140 gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg 4200 gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat 4260 gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga 4320 atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg 4380 gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc 4440 aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca 4500 ataagttgga gtcattacca aaaggttagg aatacggtta gccatttgcc tgcttttata 4560 tagttcatat gggattcacc tttatgttga taagaaataa aagaaaatgc caataggata 4620 tcggcatttt cttttgcgtt tttatttgtt aactgttaat tgtccttgtt caaggatgct 4680 gtctttgaca acagatgttt tcttgccttt gatgttcagc aggaagcttg gcgcaaacgt 4740 tgattgtttg tctgcgtaga atcctctgtt tgtcatatag cttgtaatca cgacattgtt 4800 tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag gttacatcgt taggatcaag 4860 atccattttt aacacaaggc cagttttgtt cagcggcttg tatgggccag ttaaagaatt 4920 agaaacataa ccaagcatgt aaatatcgtt agacgtaatg ccgtcaatcg tcatttttga 4980 tccgcgggag tcagtgaaca ggtaccattt gccgttcatt ttaaagacgt tcgcgcgttc 5040 aatttcatct gttactgtgt tagatgcaat cagcggtttc atcacttttt tcagtgtgta 5100 atcatcgttt agctcaatca taccgagagc gccgtttgct aactcagccg tgcgtttttt 5160 atcgctttgc agaagttttt gactttcttg acggaagaat gatgtgcttt tgccatagta 5220 tgctttgtta aataaagatt cttcgccttg gtagccatct tcagttccag tgtttgcttc 5280 aaatactaag tatttgtggc ctttatcttc tacgtagtga ggatctctca gcgtatggtt 5340 gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt acattttgat acgtttttcc 5400 gtcaccgtca aagattgatt tataatcctc tacaccgttg atgttcaaag agctgtctga 5460 tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg ccgtaatgtt taccggagaa 5520 atcagtgtag aataaacgga tttttccgtc agatgtaaat gtggctgaac ctgaccattc 5580 ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat ttgtcgctgt ctttaaagac 5640 gcggccagcg tttttccagc tgtcaataga agtttcgccg actttttgat agaacatgta 5700 aatcgatgtg tcatccgcat ttttaggatc tccggctaat gcaaagacga tgtggtagcc 5760 gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc cagctgtccc aaacgtccag 5820 gccttttgca gaagagatat ttttaattgt ggacgaatcg aattcaggaa cttgatattt 5880 ttcatttttt tgctgttcag ggatttgcag catatcatgg cgtgtaatat gggaaatgcc 5940 gtatgtttcc ttatatggct tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc 6000 tgccagcagt gcggtagtaa aggttaatac tgttgcttgt tttgcaaact ttttgatgtt 6060 catcgttcat gtctcctttt ttatgtactg tgttagcggt ctgcttcttc cagccctcct 6120 gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg 6180 acgccaaagt atacactttg ccctttacac attttaggtc ttgcctgctt tatcagtaac 6240 aaacccgcgc gatttacttt tcgacctcat tctattagac tctcgtttgg attgcaactg 6300 gtctattttc ctcttttgtt tgatagaaaa tcataaaagg atttgcagac tacgggccta 6360 aagaactaaa aaatctatct gtttcttttc attctctgta ttttttatag tttctgttgc 6420 atgggcataa agttgccttt ttaatcacaa ttcagaaaat atcataatat ctcatttcac 6480 taaataatag tgaacggcag gtatatgtga tgggttaaaa aggatcttaa ggcctaggtc 6540 tagcttggct gcaggggggg gggggggact tccattgttc attccacgga caaaaacaga 6600 gaaaggaaac gacagaggcc aaaaagcctc gctttcagca cctgtcgttt cctttctttt 6660 cagagggtat tttaaataaa aacattaagt tatgacgaag aagaacggaa acgccttaaa 6720 ccggaaaatt ttcataaata gcgaaaaccc gcgaggtcgc cgccccgtaa cctgtcggat 6780 caccggaaag gacccgtaaa gtgataatga ttatcatcta catatcacaa cgtgcgtgga 6840 ggccatcaaa ccacgtcaaa taatcaatta tgacgcaggt atcgtattaa ttgatctgca 6900 tcaacttaac gtaaaaacaa cttcagacaa tacaaatcag cgacactgaa tacggggcaa 6960 cctcatgtcc cccccccccc ctgcag 6986 //