LOCUS       pKNG101                 6986 bp    DNA     circular      8-NOV-2012
DEFINITION  LMBP 5246.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 5246
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 8-NOV-2012
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|642168362|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pKNG101|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 8 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 4 1345 0 2951 0 3839 0 
COMMENT            3842 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 445 0 2764 0 5861 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 2 21 0 5006 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 2 222 0 1239 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NsiI" "atgcat" 6 0 1 257 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 839 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 1 6729 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SmaI" "cccggg" 4 0 2 15 0 242 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 277 0 2340 0 0 "")) 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "696..2335") 
COMMENT        #11=(CFSignal (CObList) "R6K ori" 33 0 0 2373 2760 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2373..2760") 
COMMENT        #12=(CFSignal (CObList) "oriT" 33 0 1 3477 3587 0 (CStringList) 
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COMMENT        #13=(CFSignal (CObList) "insB" 4 0 0 4001 4504 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "sacB" 4 0 1 4642 6063 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pKNG101'" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6986 bp" 1) 
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COMMENT                                        "B. subtilis levansucrase coding sequence" 
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COMMENT                                        "origin of replication of plasmid R6K (defective pir gene)" 
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COMMENT                                        "RK2 conjugational DNA transfer origin" 
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FEATURES             Location/Qualifiers
     CDS             696..2335
                     /vntifkey="4"
                     /label=streptomycin\resistance
     rep_origin      2373..2760
                     /vntifkey="33"
                     /label=R6K\ori
                     /note="origin of replication of plasmid R6K (defective pir gene)"
     rep_origin      complement(3477..3587)
                     /vntifkey="33"
                     /label=oriT
                     /note="RK2 conjugational DNA transfer origin"
     CDS             4001..4504
                     /vntifkey="4"
                     /label=insB
                     /note="E. coli insertion sequence IS1 insB"
     CDS             complement(4642..6063)
                     /vntifkey="4"
                     /label=sacB
                     /note="B. subtilis levansucrase coding sequence"
BASE COUNT     1641 a      1677 c      1765 g      1903 t 
ORIGIN
        1 gtcgacggat ccccgggtac cataagtaga agcagcaacc caagtagctt taccagcatc 
       61 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc 
      121 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct 
      181 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc 
      241 cgggcccgcg gatgcatatg atcttaaggc ctaggtctag agtctttgtt ttgacgccat 
      301 tagcgtacgt aacaatcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt 
      361 agacggagta tactagtata gtctatagtc cgtggaatta ttatatttat ctccgacgat 
      421 attctcatca gtgaaatcca ggggaattct catgtttgac agcttatcat cgataagctt 
      481 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct 
      541 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg 
      601 gttatgccgg tactgccggg cctcttgcgg gataactgca cattcgggat atttctctat 
      661 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg 
      721 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc 
      781 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg 
      841 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca 
      901 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac 
      961 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac 
     1021 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa 
     1081 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg 
     1141 acgaggacaa gagtacgccg ctgcacgatc ttttggctcg tgtcgaacga gagctaccgg 
     1201 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga 
     1261 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg 
     1321 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg 
     1381 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag 
     1441 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat 
     1501 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga 
     1561 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa 
     1621 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg 
     1681 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga 
     1741 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac 
     1801 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc 
     1861 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca 
     1921 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag 
     1981 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc 
     2041 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc 
     2101 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat 
     2161 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga 
     2221 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca 
     2281 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc 
     2341 tagatccggc ccgatgcgtc cggcgtagag gatctgaaga tcagcagttc aacctgttga 
     2401 tagtacgtac taagctctca tgtttcacgt actaagctct catgtttaac gtactaagct 
     2461 ctcatgttta acgaactaaa ccctcatggc taacgtacta agctctcatg gctaacgtac 
     2521 taagctctca tgtttcacgt actaagctct catgtttgaa caataaaatt aatataaatc 
     2581 agcaacttaa atagcctcta aggttttaag ttttataaga aaaaaaagaa tatataaggc 
     2641 ttttaaagct tttaaggttt aacggttgtg gacaacaagc cagggatgta acgcactgag 
     2701 aagcccttag agcctctcaa agcaattttc agtgacacag gaacacttaa cggctgacat 
     2761 gggaattcta ctgtttgggt gtatgagcta tttgcaaagg aaattggtga tgacaaagct 
     2821 cggcgctatt tgaagaaaat cgactatggc aacgccgatc ctttttgtcc ggtgttgggt 
     2881 tgaaggtgaa gccggtcggg gccgcagcgg gggccggctt ttcagccttg cccccctgct 
     2941 tcggccgccg tggctccggc gtcttgggtg ccggcgcggg ttccgcagcc ttggcctgcg 
     3001 gtgcgggcac atcggcgggc ttggccttga tgtgccgcct ggcgtgcgag cggaacgtct 
     3061 cgtaggagaa cttgaccttc cccgtttccc gcatgtgctc ccaaatggtg acgagcgcat 
     3121 agccggacgc taacgccgcc tcgacatccg ccctcaccgc caggaacgca accgcagcct 
     3181 catcacgccg gcgcttcttg gccgcgcggg attcaaccca ctcggccagc tcgtcggtgt 
     3241 agctctttgg catcgtctct cgcctgtccc ctcagttcag taatttcctg catttgcctg 
     3301 tttccagtcg gtagatattc cacaaaacag cagggaagca gcgcttttcc gctgcataac 
     3361 cctgcttcgg ggtcattata gcgatttttt cggtatatcc atcctttttc gcacgatata 
     3421 caggattttg ccaaagggtt cgtgtagact ttccttggtg tatccaacgg cgtcagccgg 
     3481 gcaggatagg tgaagtaggc ccacccgcga gcgggtgttc cttcttcact gtcccttatt 
     3541 cgcacctggc ggtgctcaac gggaatcctg ctctgcgagg ctggccggct accgccggcg 
     3601 taacagatga gggcaagcgg atggctgatg aaaccaagcc aaccaggaag ggcagcccac 
     3661 ctatcaaggt gtactgcctt ccagacgaac gaagagcgat tgaggaaaag gcggcggcgg 
     3721 ccggcatgag cctgtcggcc tacctgctgg ccgtcggcca gggctacaaa atcacgggcg 
     3781 tcgtggacta tgagcacgtc cgcgagctgg cccgcatcaa tggcgacctg ggccgcctgg 
     3841 gcggcctgct gaaactctgg ctcaccgacg acccgcgcac ggcgcggttc ggtgatgcca 
     3901 cgatccgggc aacgttgttg ccattgctgc agttcactta caccgcttct caacccggta 
     3961 cgcaccataa aatcattgat atggccatga atggcgttgg atgccgggca acagcccgca 
     4021 ttatgggcgt tggcctcaac acgattttac gtcacttaaa aaactcaggc cgcagtcggt 
     4081 aacctcgcgc atacagccgg gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg 
     4141 gggctatgtc ggggctaaat cgcgccagcg ctggctgttt tacgcgtatg acagtctccg 
     4201 gaagacggtt gttgcgcacg tattcggtga acgcactatg gcgacgctgg ggcgtcttat 
     4261 gagcctgctg tcaccctttg acgtggtgat atggatgacg gatggctggc cgctgtatga 
     4321 atcccgcctg aagggaaagc tgcacgtaat cagcaagcga tatacgcagc gaattgagcg 
     4381 gcataacctg aatctgaggc agcacctggc acggctggga cggaagtcgc tgtcgttctc 
     4441 aaaatcggtg gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca 
     4501 ataagttgga gtcattacca aaaggttagg aatacggtta gccatttgcc tgcttttata 
     4561 tagttcatat gggattcacc tttatgttga taagaaataa aagaaaatgc caataggata 
     4621 tcggcatttt cttttgcgtt tttatttgtt aactgttaat tgtccttgtt caaggatgct 
     4681 gtctttgaca acagatgttt tcttgccttt gatgttcagc aggaagcttg gcgcaaacgt 
     4741 tgattgtttg tctgcgtaga atcctctgtt tgtcatatag cttgtaatca cgacattgtt 
     4801 tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag gttacatcgt taggatcaag 
     4861 atccattttt aacacaaggc cagttttgtt cagcggcttg tatgggccag ttaaagaatt 
     4921 agaaacataa ccaagcatgt aaatatcgtt agacgtaatg ccgtcaatcg tcatttttga 
     4981 tccgcgggag tcagtgaaca ggtaccattt gccgttcatt ttaaagacgt tcgcgcgttc 
     5041 aatttcatct gttactgtgt tagatgcaat cagcggtttc atcacttttt tcagtgtgta 
     5101 atcatcgttt agctcaatca taccgagagc gccgtttgct aactcagccg tgcgtttttt 
     5161 atcgctttgc agaagttttt gactttcttg acggaagaat gatgtgcttt tgccatagta 
     5221 tgctttgtta aataaagatt cttcgccttg gtagccatct tcagttccag tgtttgcttc 
     5281 aaatactaag tatttgtggc ctttatcttc tacgtagtga ggatctctca gcgtatggtt 
     5341 gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt acattttgat acgtttttcc 
     5401 gtcaccgtca aagattgatt tataatcctc tacaccgttg atgttcaaag agctgtctga 
     5461 tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg ccgtaatgtt taccggagaa 
     5521 atcagtgtag aataaacgga tttttccgtc agatgtaaat gtggctgaac ctgaccattc 
     5581 ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat ttgtcgctgt ctttaaagac 
     5641 gcggccagcg tttttccagc tgtcaataga agtttcgccg actttttgat agaacatgta 
     5701 aatcgatgtg tcatccgcat ttttaggatc tccggctaat gcaaagacga tgtggtagcc 
     5761 gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc cagctgtccc aaacgtccag 
     5821 gccttttgca gaagagatat ttttaattgt ggacgaatcg aattcaggaa cttgatattt 
     5881 ttcatttttt tgctgttcag ggatttgcag catatcatgg cgtgtaatat gggaaatgcc 
     5941 gtatgtttcc ttatatggct tttggttcgt ttctttcgca aacgcttgag ttgcgcctcc 
     6001 tgccagcagt gcggtagtaa aggttaatac tgttgcttgt tttgcaaact ttttgatgtt 
     6061 catcgttcat gtctcctttt ttatgtactg tgttagcggt ctgcttcttc cagccctcct 
     6121 gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga cctaaaatat gtaaggggtg 
     6181 acgccaaagt atacactttg ccctttacac attttaggtc ttgcctgctt tatcagtaac 
     6241 aaacccgcgc gatttacttt tcgacctcat tctattagac tctcgtttgg attgcaactg 
     6301 gtctattttc ctcttttgtt tgatagaaaa tcataaaagg atttgcagac tacgggccta 
     6361 aagaactaaa aaatctatct gtttcttttc attctctgta ttttttatag tttctgttgc 
     6421 atgggcataa agttgccttt ttaatcacaa ttcagaaaat atcataatat ctcatttcac 
     6481 taaataatag tgaacggcag gtatatgtga tgggttaaaa aggatcttaa ggcctaggtc 
     6541 tagcttggct gcaggggggg gggggggact tccattgttc attccacgga caaaaacaga 
     6601 gaaaggaaac gacagaggcc aaaaagcctc gctttcagca cctgtcgttt cctttctttt 
     6661 cagagggtat tttaaataaa aacattaagt tatgacgaag aagaacggaa acgccttaaa 
     6721 ccggaaaatt ttcataaata gcgaaaaccc gcgaggtcgc cgccccgtaa cctgtcggat 
     6781 caccggaaag gacccgtaaa gtgataatga ttatcatcta catatcacaa cgtgcgtgga 
     6841 ggccatcaaa ccacgtcaaa taatcaatta tgacgcaggt atcgtattaa ttgatctgca 
     6901 tcaacttaac gtaaaaacaa cttcagacaa tacaaatcag cgacactgaa tacggggcaa 
     6961 cctcatgtcc cccccccccc ctgcag 
//
