ID pPTB-HcRED standard; circular DNA; ; 6218 BP. DT 29-OCT-2007 DE LMBP 5218. CC Accession number: LMBP 5218 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS 639..2309 FT /vntifkey="4" FT /label=hPTB FT /note="Human polypyrimidine tract binding protein" FT CDS 2316..3002 FT /vntifkey="4" FT /label=HcRed1 FT /note="Far-red fluorescent protein; coding sequence" FT rep_origin 3979..4054 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 1..568 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT /note="Human cytomegalovirus immediate early promoter and enhancer" FT rep_origin 5886..6212 FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS 4114..4908 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT rep_origin complement(3134..3589) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT terminator 5099..5168 FT /vntifkey="43" FT /label=HSV-TK\polyA FT /note="Herpes simplex virus (HSV) thymidine kinase polyadenylation signal" FT promoter 3741..4022 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter (SV40 early)" FT promoter 3590..3685 FT /vntifkey="30" FT /label=amp\promoter FT /note="Escherichia coli ß-lactamase promoter" FT RBS 3686..3720 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT /note="5' UTR of the ampicillin resistance gene, incl. RBS" SQ Sequence 6218 BP; 1440 A; 1787 C; 1680 G; 1311 t; tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 120 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600 ccggactcag atctcgagct caagcttcga attccaccat ggacggcatt gtcccagata 660 tagccgttgg tacaaagcgg ggatctgacg agcttttctc tacttgtgtc actaacggac 720 cgtttatcat gagcagcaac tcggcttctg cagcaaacgg aaatgacagc aagaagttca 780 aaggtgacag ccgaagtgca ggcgtcccct ctagagtgat ccacatccgg aagctcccca 840 tcgacgtcac ggagggggaa gtcatctccc tggggctgcc ctttgggaag gtcaccaacc 900 tcctgatgct gaaggggaaa aaccaggcct tcatcgagat gaacacggag gaggctgcca 960 acaccatggt gaactactac acctcggtga cccctgtgct gcgcggccag cccatctaca 1020 tccagttctc caaccacaag gagctgaaga ccgacagctc tcccaaccag gcgcgggccc 1080 aggcggccct gcaggcggtg aactcggtcc agtcggggaa cctggccttg gctgcctcgg 1140 cggcggccgt ggacgcaggg atggcgatgg ccgggcagag ccccgtgctc aggatcatcg 1200 tggagaacct cttctaccct gtgaccctgg atgtgctgca ccagattttc tccaagttcg 1260 gcacagtgtt gaagatcatc accttcacca agaacaacca gttccaggcc ctgctgcagt 1320 atgcggaccc cgtgagcgcc cagcacgcca agctgtcgct ggacgggcag aacatctaca 1380 acgcctgctg cacgctgcgc atcgactttt ccaagctcac cagcctcaac gtcaagtaca 1440 acaatgacaa gagccgtgac tacacacgcc cagacctgcc ttccggggac agccagccct 1500 cgctggacca gaccatggcc gcggccttcg gtgcacctgg tataatctca gcctctccgt 1560 atgcaggagc tggtttccct cccacctttg ccattcctca agctgcaggc ctttccgttc 1620 cgaacgtcca cggcgccctg gcccccctgg ccatcccctc ggcggcggcg gcagctgcgg 1680 cggcaggtcg gatcgccatc ccgggcctgg cgggggcagg aaattctgta ttgctggtca 1740 gcaacctcaa cccagagaga gtcacacccc aaagcctctt tattcttttc ggcgtctacg 1800 gtgacgtgca gcgcgtgaag atcctgttca ataagaagga gaacgcccta gtgcagatgg 1860 cggacggcaa ccaggcccag ctggccatga gccacctgaa cgggcacaag ctgcacggga 1920 agcccatccg catcacgctc tcgaagcacc agaacgtgca gctgccccgc gagggccagg 1980 aggaccaggg cctgaccaag gactacggca actcacccct gcaccgcttc aagaagccgg 2040 gctccaagaa cttccagaac atattcccgc cctcggccac gctgcacctc tccaacatcc 2100 cgccctcagt ctccgaggag gatctcaagg tcctgttttc cagcaatggg ggcgtcgtca 2160 aaggattcaa gttcttccag aaggaccgca agatggcact gatccagatg ggctccgtgg 2220 aggaggcggt ccaggccctc attgacctgc acaaccacga cctcggggag aaccaccacc 2280 tgcgggtctc cttctccaag tccaccatcg gatccatggt gagcggcctg ctgaaggaga 2340 gtatgcgcat caagatgtac atggagggca ccgtgaacgg ccactacttc aagtgcgagg 2400 gcgagggcga cggcaacccc ttcgccggca cccagagcat gagaatccac gtgaccgagg 2460 gcgcccccct gcccttcgcc ttcgacatcc tggccccctg ctgcgagtac ggcagcagga 2520 ccttcgtgca ccacaccgcc gagatccccg acttcttcaa gcagagcttc cccgagggct 2580 tcacctggga gagaaccacc acctacgagg acggcggcat cctgaccgcc caccaggaca 2640 ccagcctgga gggcaactgc ctgatctaca aggtgaaggt gcacggcacc aacttccccg 2700 ccgacggccc cgtgatgaag aacaagagcg gcggctggga gcccagcacc gaggtggtgt 2760 accccgagaa cggcgtgctg tgcggccgga acgtgatggc cctgaaggtg ggcgaccggc 2820 acctgatctg ccaccactac accagctacc ggagcaagaa ggccgtgcgc gccctgacca 2880 tgcccggctt ccacttcacc gacatccggc tccagatgct gcggaagaag aaggacgagt 2940 acttcgagct gtacgaggcc agcgtggccc ggtacagcga cctgcccgag aaggccaact 3000 gagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 3060 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 3120 tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 3180 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 3240 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 3300 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 3360 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 3420 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 3480 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 3540 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact 3600 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3660 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3720 cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag 3780 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 3840 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 3900 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 3960 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 4020 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag 4080 atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca 4140 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc 4200 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc 4260 aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg 4320 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg 4380 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct 4440 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct 4500 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa 4560 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa 4620 ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc 4680 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt 4740 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct 4800 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc 4860 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg 4920 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg 4980 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc 5040 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa 5100 cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata 5160 aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact 5220 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc 5280 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag 5340 gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt 5400 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 5460 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 5520 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 5580 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 5640 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 5700 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 5760 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 5820 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 5880 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 5940 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 6000 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 6060 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 6120 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 6180 cccctgattc tgtggataac cgtattaccg ccatgcat 6218 //