ID pMSK1-E-D565A standard; circular DNA; ; 6575 BP. DT 11-SEP-2008 DE LMBP 4982 CC Accession number: LMBP 4982 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 11-SEP-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT rep_origin complement(36..111) FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(262..374) FT /vntifkey="33" FT /label=polyoma\ori FT /note="origin polyoma and enhancer" FT terminator complement(1066..1162) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 1066..1162 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT misc_feature complement(1163..1561) FT /vntifkey="21" FT /label=3'\UTR\of\SV40\small\t-antigen FT /note="3' UTR of the SV40 small t-antigen" FT intron complement(1562..1628) FT /vntifkey="15" FT /label=intron\of\SV40\small\t-antigen FT /note="intron of the SV40 small t-antigen" FT CDS complement(1629..1703) FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT /note="part of the SV40 small t-antigen" FT promoter 1711..1727 FT /vntifkey="30" FT /label=SP6\promoter FT /note="phage SP6 promoter" FT misc_marker complement(1871..1909) FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS complement(1919..4324) FT /vntifkey="4" FT /label=hMSK1\mutant FT /note="mutated human MSK1 coding sequence" FT promoter complement(4390..4406) FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT promoter complement(4451..5034) FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT /note="human CMV-IE promoter and enhancer" FT tRNA complement(5189..5391) FT /vntifkey="59" FT /label=supF FT /note="suppressor tRNA gene, incl. own promoter" FT rep_origin 5515..5973 FT /vntifkey="33" FT /label=M13\ori FT /note="phage M13 origin" FT rep_origin complement(5992..6224) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT mutation complement(2630..2632) FT /vntifkey="62" FT /label=D565A FT /note="wt: GAT" SQ Sequence 6575 BP; 1668 A; 1566 C; 1494 G; 1847 t; gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata 60 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg 120 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac 180 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag 240 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca 300 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat 360 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg 420 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac 480 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tgcttaactg 540 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctccgcc 600 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta 660 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct 720 attttatctg gagaagccct cgctgccctt gatggcgaaa ttacagctct gactttggag 780 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg 840 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag 900 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag 960 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatgatcc agacatgata 1020 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 1080 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 1140 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 1200 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac 1260 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg 1320 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta 1380 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag 1440 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca 1500 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa 1560 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta 1620 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga 1680 agtaaggttc cttcacaaag atcctctagc atttaggtga cactatagaa tagggccctc 1740 tagagtcgac attccacaga cagccctcat agttagcgta acgatctaaa gttttgtcgt 1800 ctttccagac gttagtaaat gaattttctg tatgaggttt tgctaaacaa ctttcaacag 1860 tctatgcggc acgcggttcc agcggatccg gatacggcac cggcgcacct gcggccgcag 1920 ctactgagtc cgagaactgg aagagggtct ccgggttatt gctgtcggca ggattgctgg 1980 gctgcagtgt cttggtgggt gtagttttac cgtgagaatg agaggaagaa gaatgggaac 2040 tctcactgga actgctgcgc gtctcggtac tggtgctagt ctttttcatt tttcttctct 2100 tagccaaagg ggccttatca acattctgaa ggcaaaaccc ctctctcttg tatttgttaa 2160 aggcgtggaa ggttgctttc acacaggtat gcacggcagc tccggaagat cctagaatat 2220 ccggagtcat cagaggattg gaggacagct gacttccatc ttgtagccat tcattgtacc 2280 tcaagccaga cattttaagc cttttgtttg gatctactgt gagaagtcct tggatcaaat 2340 ctttagcctc ttgggataca ttcttccagg cttctccttc aaaggagaaa tctccctttt 2400 taattttctt catgatttcc accgcgctgg tacacgtcaa acttcggtca tgagattgga 2460 agggaacctg tcctgacaac attgtgtaca aaatgacgcc caagctccac aggtcacagg 2520 actcatcgta gccgttctga ttcaagagct ctggggcggc ataatgaagg gtgaagcatg 2580 gagtcttcag gggctgatta tccggtggct ttagccgtgc aaatccaaaa gcaattattt 2640 taatttccaa attgtcattt tcatcggtga acaataaatt ctcaggtttc agatccctgt 2700 gcaccactcc aacatcatgc atgtggctta cagctgaaac aagcttcctc atgatgtagc 2760 tggcttccgt ctcactgaag tgcttctttt tcttaatgcg ctcaaacagt tctcctccat 2820 tcagaagttc catcactaga aacgtgtgaa gctgatcatg aaaaacttca tgcaacttca 2880 caatattggg gtgtccttca cagagtttca gagctgttat ttccttttga gtattggctt 2940 ccatcctttt gctgattatt ttgactgcaa aagcttggtt acttttttta tgcacacact 3000 ttcgacaaat tgaaaaacta ccttctccca ggggtttgtc cttcaaatct aggtcatagt 3060 gttgatagaa tggagagtcc ttcatcattg cactcctggc aacatttgtc actccaggac 3120 gttcaactcc catgtgaaac tgaagagggt ctatgacagc tgcattacgc ttgaatagga 3180 tggaaggagc aacaaaggaa tagccctgaa acagcttctc agaactctgg ggcagggctg 3240 cgggagaata agtgggatcc atttctgtga actcttctgc aaagttactc acatctaatt 3300 catctcgaat gactggctta aatggtgcag gcactttttt ggcggctaaa tcatcccaat 3360 ttattttctg aaagaagaga tgttctttga tttcatctgc atcacgtgga ccacatccca 3420 atctcttctt gggatctttc atcaaaagac gctgaattag gtctttcgct aaagcactca 3480 tttcttgggg atatggaggc tcacttttta atattctcct agatatctca gcttgggaat 3540 ttttttctcc atcaacagtg aaaggagatg ctccagttag taattcatac attagaacac 3600 ccaaactcca ccagtcaact gccttgtcat gtcctgaatc tccccctctg acaatatctg 3660 gtgccatgta ttcaatagtt ccacaaaagg aatatgctct ttcagtttca tcagccacaa 3720 actccttact cagaccaaaa tctgtcagca ccacatggcc attagaatca agtagaatat 3780 tctcaagctt aatatcacga tatataatcc ccaacttgtg gagatgttcg agggcaagca 3840 caatctctcc aacataaatc tgcacctcat gctctgtgaa acgctctctt tgagaaagat 3900 gagtaaaaag ttcaccacca tttatataat ctaaaatgag atgaagtttg gtttctgtct 3960 ggaaagcata atgtaatgtt accaaaaatg gcgactgcct aatgtgttcc aggacttgtc 4020 gttctgtcct tgtatgctct gtggttttgg ccttttgaac gattgttgcc tttttcaaaa 4080 ctttcatggc atacagcttt ccagtatcat ggccacttat tttacgaact agaaatactt 4140 ttccataagc tccagttcct aggaccttca ggagctcaaa attttctatt cccaccttct 4200 cagcatgtcc tgtcaaatta gcagtccgca gctcgtgctt gacagtgagg agctgctctc 4260 ctccgtcgcc gccgtccgcg ctggtccccg cggcgccgcc gctgctgcca ccctcctcct 4320 ccatggaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt 4380 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag 4440 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg 4500 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca 4560 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc 4620 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt 4680 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg 4740 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta 4800 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta 4860 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg 4920 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat 4980 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg 5040 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca 5100 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttactcgcg cactactcag 5160 cgacctccaa cacacaagca gggagcagat tctttcggac ttttgaaagt gatggtggtg 5220 ggggaaggat tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc 5280 cgctcgggaa ccccaccacg ggtaatgctt ttactggcct gctcccttat cgggaagcgg 5340 ggcgcatcat atcaaatgac gcgccgctgt aaagtgttac gttgagaaag accgcggtaa 5400 tccagctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg 5460 ctctgattcc aacgaggaaa gcacgttata cggtctcgtc aaagcaacca tagtacgcgc 5520 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 5580 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 5640 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 5700 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat 5760 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 5820 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 5880 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 5940 cgaattttaa caaaatatta acgtttacaa tttctagcta gaagctagct tgcgttgctg 6000 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 6060 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 6120 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 6180 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 6240 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 6300 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 6360 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 6420 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 6480 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 6540 ggtggttttt ttgtttgcaa gcagcagatt acgcc 6575 //