LOCUS       pMSK1-E-D565A           6575 bp    DNA     circular     11-SEP-2008
DEFINITION  LMBP 4982.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4982
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 11-SEP-2008
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|493129044|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMSK1-E-D565A|
COMMENT     VNTAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMSK1-E-D565A" 0 6575 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        (CWordArray) (CStringList "EcoRI" "HindIII" "NotI" "BsrDI" "DraIII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4327 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 4 2742 0 2972 0 3786 0 
COMMENT            4376 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1913 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 1 3086 1 0 "") 
COMMENT        #4=(CRSite (CStringList) "DraIII" "cacnnngtg" 7 0 1 5749 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "complement(36..111)") 
COMMENT        #6=(CFSignal (CObList) "polyoma ori" 33 0 1 262 374 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(262..374)") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA early" 43 0 1 1066 1162 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1066..1162)") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA late" 43 0 0 1066 1162 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1066..1162") 
COMMENT        #9=(CFSignal (CObList) "3' UTR of SV40 small t-antigen" 21 0 1 1163 1561 
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COMMENT        #12=(CFSignal (CObList) "SP6 promoter" 30 0 0 1711 1727 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1711..1727") 
COMMENT        #13=(CFSignal (CObList) "hMSK1 mutant" 4 0 1 1919 4324 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1919..4324)") 
COMMENT        #14=(CFSignal (CObList) "T7 promoter" 30 0 1 4390 4406 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 1 4451 5034 
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COMMENT        #16=(CFSignal (CObList) "M13 ori" 33 0 0 5515 5973 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 5992 6224 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "supF" 59 0 1 5189 5391 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "D565A" 62 0 1 2630 2632 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pMSK1-E-D565A'" 1) 
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COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6575 bp" 1) 
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COMMENT                   "Standard Fields") 
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COMMENT                                 "hCMV-IE promoter and enhancer"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "   complement(4390..4406)" 1))) 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                           "suppressor tRNA gene, incl. own promoter" 
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FEATURES             Location/Qualifiers
     rep_origin      complement(36..111)
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     rep_origin      complement(262..374)
                     /vntifkey="33"
                     /label=polyoma\ori
                     /note="origin polyoma and enhancer"
     terminator      complement(1066..1162)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      1066..1162
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     misc_feature    complement(1163..1561)
                     /vntifkey="21"
                     /label=3'\UTR\of\SV40\small\t-antigen
                     /note="3' UTR of the SV40 small t-antigen"
     intron          complement(1562..1628)
                     /vntifkey="15"
                     /label=intron\of\SV40\small\t-antigen
                     /note="intron of the SV40 small t-antigen"
     CDS             complement(1629..1703)
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
                     /note="part of the SV40 small t-antigen"
     promoter        1711..1727
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="phage SP6 promoter"
     misc_marker     complement(1871..1909)
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             complement(1919..4324)
                     /vntifkey="4"
                     /label=hMSK1\mutant
                     /note="mutated human MSK1 coding sequence"
     promoter        complement(4390..4406)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     promoter        complement(4451..5034)
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
                     /note="human CMV-IE promoter and enhancer"
     tRNA            complement(5189..5391)
                     /vntifkey="59"
                     /label=supF
                     /note="suppressor tRNA gene, incl. own promoter"
     rep_origin      5515..5973
                     /vntifkey="33"
                     /label=M13\ori
                     /note="phage M13 origin"
     rep_origin      complement(5992..6224)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     mutation        complement(2630..2632)
                     /vntifkey="62"
                     /label=D565A
                     /note="wt: GAT"
BASE COUNT     1668 a      1566 c      1494 g      1847 t 
ORIGIN
        1 gaattagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata 
       61 gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt cagccatggg 
      121 gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagta agacccgcac 
      181 ccgttcccaa actgcttatt aaagggggta tggaggtgct ggaccttgtg acagggccag 
      241 acagtgtgac agaaatagaa gcagtcctgt tttgacaagt tgcctctgga agcctctaca 
      301 atgcctctct tctttttctc cagagtaagc gggcccaggg gtccccggcc tctgcttaat 
      361 actaaaaaaa acagctgttg tcatagtaat gattgggtgg aaacattcta ggcctgggtg 
      421 gagaggcttt tgcttcctct tgcaaaacca cacgccctct ggagggcgtt gcctagcaac 
      481 taattaaaag aggatgtcgc acggccagct gcgtcagtta gtccacttcc tgcttaactg 
      541 acttgacatt ttctatttta agagtcggga ggaaaattac tgtgttggag gccctccgcc 
      601 atcttctgaa gctgatcaag taagtgaatt ttcaaaatgg gagccgcact gactattcta 
      661 gtagacctca tcgagggatt agctgaagtg tctaccctta cgggactctc ggcagaagct 
      721 attttatctg gagaagccct cgctgccctt gatggcgaaa ttacagctct gactttggag 
      781 ggtgtaatga gttcggagac agccctagca actatgggta tttcagagga ggtgtatggg 
      841 tttgtcagta ctgtgcctgt gtttgtaagt cgaacagcgg gggctatatg gctgatgcag 
      901 acagttcaag gtgcctctac tatttcccta ggaatacagc ggtacctaca caacgaagag 
      961 gtccctactg taaatagaaa tatggcgttg ataccatggc gggatgatcc agacatgata 
     1021 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt 
     1081 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt 
     1141 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt 
     1201 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatctcta gtcaaggcac 
     1261 tatacatcaa atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg 
     1321 tgtaaatagc aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta 
     1381 gcaattctga aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag 
     1441 aatgttgaga gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca 
     1501 ggttttcctc attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa 
     1561 tctaaaatac acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta 
     1621 tatttacctt agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga 
     1681 agtaaggttc cttcacaaag atcctctagc atttaggtga cactatagaa tagggccctc 
     1741 tagagtcgac attccacaga cagccctcat agttagcgta acgatctaaa gttttgtcgt 
     1801 ctttccagac gttagtaaat gaattttctg tatgaggttt tgctaaacaa ctttcaacag 
     1861 tctatgcggc acgcggttcc agcggatccg gatacggcac cggcgcacct gcggccgcag 
     1921 ctactgagtc cgagaactgg aagagggtct ccgggttatt gctgtcggca ggattgctgg 
     1981 gctgcagtgt cttggtgggt gtagttttac cgtgagaatg agaggaagaa gaatgggaac 
     2041 tctcactgga actgctgcgc gtctcggtac tggtgctagt ctttttcatt tttcttctct 
     2101 tagccaaagg ggccttatca acattctgaa ggcaaaaccc ctctctcttg tatttgttaa 
     2161 aggcgtggaa ggttgctttc acacaggtat gcacggcagc tccggaagat cctagaatat 
     2221 ccggagtcat cagaggattg gaggacagct gacttccatc ttgtagccat tcattgtacc 
     2281 tcaagccaga cattttaagc cttttgtttg gatctactgt gagaagtcct tggatcaaat 
     2341 ctttagcctc ttgggataca ttcttccagg cttctccttc aaaggagaaa tctccctttt 
     2401 taattttctt catgatttcc accgcgctgg tacacgtcaa acttcggtca tgagattgga 
     2461 agggaacctg tcctgacaac attgtgtaca aaatgacgcc caagctccac aggtcacagg 
     2521 actcatcgta gccgttctga ttcaagagct ctggggcggc ataatgaagg gtgaagcatg 
     2581 gagtcttcag gggctgatta tccggtggct ttagccgtgc aaatccaaaa gcaattattt 
     2641 taatttccaa attgtcattt tcatcggtga acaataaatt ctcaggtttc agatccctgt 
     2701 gcaccactcc aacatcatgc atgtggctta cagctgaaac aagcttcctc atgatgtagc 
     2761 tggcttccgt ctcactgaag tgcttctttt tcttaatgcg ctcaaacagt tctcctccat 
     2821 tcagaagttc catcactaga aacgtgtgaa gctgatcatg aaaaacttca tgcaacttca 
     2881 caatattggg gtgtccttca cagagtttca gagctgttat ttccttttga gtattggctt 
     2941 ccatcctttt gctgattatt ttgactgcaa aagcttggtt acttttttta tgcacacact 
     3001 ttcgacaaat tgaaaaacta ccttctccca ggggtttgtc cttcaaatct aggtcatagt 
     3061 gttgatagaa tggagagtcc ttcatcattg cactcctggc aacatttgtc actccaggac 
     3121 gttcaactcc catgtgaaac tgaagagggt ctatgacagc tgcattacgc ttgaatagga 
     3181 tggaaggagc aacaaaggaa tagccctgaa acagcttctc agaactctgg ggcagggctg 
     3241 cgggagaata agtgggatcc atttctgtga actcttctgc aaagttactc acatctaatt 
     3301 catctcgaat gactggctta aatggtgcag gcactttttt ggcggctaaa tcatcccaat 
     3361 ttattttctg aaagaagaga tgttctttga tttcatctgc atcacgtgga ccacatccca 
     3421 atctcttctt gggatctttc atcaaaagac gctgaattag gtctttcgct aaagcactca 
     3481 tttcttgggg atatggaggc tcacttttta atattctcct agatatctca gcttgggaat 
     3541 ttttttctcc atcaacagtg aaaggagatg ctccagttag taattcatac attagaacac 
     3601 ccaaactcca ccagtcaact gccttgtcat gtcctgaatc tccccctctg acaatatctg 
     3661 gtgccatgta ttcaatagtt ccacaaaagg aatatgctct ttcagtttca tcagccacaa 
     3721 actccttact cagaccaaaa tctgtcagca ccacatggcc attagaatca agtagaatat 
     3781 tctcaagctt aatatcacga tatataatcc ccaacttgtg gagatgttcg agggcaagca 
     3841 caatctctcc aacataaatc tgcacctcat gctctgtgaa acgctctctt tgagaaagat 
     3901 gagtaaaaag ttcaccacca tttatataat ctaaaatgag atgaagtttg gtttctgtct 
     3961 ggaaagcata atgtaatgtt accaaaaatg gcgactgcct aatgtgttcc aggacttgtc 
     4021 gttctgtcct tgtatgctct gtggttttgg ccttttgaac gattgttgcc tttttcaaaa 
     4081 ctttcatggc atacagcttt ccagtatcat ggccacttat tttacgaact agaaatactt 
     4141 ttccataagc tccagttcct aggaccttca ggagctcaaa attttctatt cccaccttct 
     4201 cagcatgtcc tgtcaaatta gcagtccgca gctcgtgctt gacagtgagg agctgctctc 
     4261 ctccgtcgcc gccgtccgcg ctggtccccg cggcgccgcc gctgctgcca ccctcctcct 
     4321 ccatggaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg taccaagctt 
     4381 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag 
     4441 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg 
     4501 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca 
     4561 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc 
     4621 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt 
     4681 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg 
     4741 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta 
     4801 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta 
     4861 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg 
     4921 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat 
     4981 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg 
     5041 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca 
     5101 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttactcgcg cactactcag 
     5161 cgacctccaa cacacaagca gggagcagat tctttcggac ttttgaaagt gatggtggtg 
     5221 ggggaaggat tcgaaccttc gaagtcgatg acggcagatt tagagtctgc tccctttggc 
     5281 cgctcgggaa ccccaccacg ggtaatgctt ttactggcct gctcccttat cgggaagcgg 
     5341 ggcgcatcat atcaaatgac gcgccgctgt aaagtgttac gttgagaaag accgcggtaa 
     5401 tccagctggc gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg 
     5461 ctctgattcc aacgaggaaa gcacgttata cggtctcgtc aaagcaacca tagtacgcgc 
     5521 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 
     5581 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 
     5641 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 
     5701 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggcggccat 
     5761 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     5821 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 
     5881 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     5941 cgaattttaa caaaatatta acgtttacaa tttctagcta gaagctagct tgcgttgctg 
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     6061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     6121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     6181 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     6241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     6301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     6361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     6421 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     6481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     6541 ggtggttttt ttgtttgcaa gcagcagatt acgcc 
//
