LOCUS       p1168hIL6mIgkB-luc+        5982 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 4736.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4736
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|461003665|
COMMENT     LSOWNER|
COMMENT     VNTNAME|p1168hIL6mIgkB-luc+|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "p1168hIL6mIgkB-luc+" 0 5982 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT         "SmaI" "SphI" "StyI" "XbaI" "XhoI" "NgoMIV" "XmaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5982 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 32 0 3173 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 2561 0 3071 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1222 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1255 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "ScaI" "agtact" 4 0 3 1422 0 4798 0 5881 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 29 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 1920 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "StyI" "ccwwgg" 2 0 2 460 0 1255 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2911 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "NgoMIV" "gccggc" 2 0 3 2926 0 3293 0 5362 0 0 
COMMENT             "") #13=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 27 0 0 "")) 
COMMENT       (CObList 
COMMENT        #14=(CFSignal (CObList) "5' FR of hIL6" 21 0 0 32 672 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "32..672") 
COMMENT        #15=(CFSignal (CObList) "hIL6 promoter mutant" 29 0 0 673 1208 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "673..1208") 
COMMENT        #16=(CFSignal (CObList) "LUCm" 4 0 0 1256 2908 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1256..2908") 
COMMENT        #17=(CFSignal (CObList) "SV40 polyA late" 43 0 0 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2996..3092") 
COMMENT        #18=(CFSignal (CObList) "SV40 polyA early" 43 0 1 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2996..3092)") 
COMMENT        #19=(CFSignal (CObList) "pMB1 ori" 33 0 1 3301 3703 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3301..3703)") 
COMMENT        #20=(CFSignal (CObList) "ampicillin" 4 0 1 4244 5104 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4244..5104)") 
COMMENT        #21=(CFSignal (CObList) "f1 ori" 33 0 0 5236 5691 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5236..5691") 
COMMENT        #22=(CFSignal (CObList) "synthetic polyA" 43 0 1 5822 5975 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5822..5975)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "LMBP 4736" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "Last modified: 23-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                    "p1168hIL6mIgkB-luc+" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid 'p1168hIL6mIgkB-luc+'" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4736." 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/07 04:34" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5982 bp" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4736") 
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COMMENT                                 "" 1))) "Last modified: 23-OCT-2007"))) 
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COMMENT                                        "Start: 1256  End: 2908" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated firefly luciferase coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
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COMMENT                                        "   1256..2908" 1))) "LUCm") 
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COMMENT                                        1) 
COMMENT                                   #53=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "   complement(4244..5104)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
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COMMENT                                        "5' flanking region of the human interleukin 6 gene" 
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COMMENT                                        "Start: 673   End: 1208" 1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                        "mutated human interleukin 6 promoter" 1) 
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COMMENT                                 "hIL6 promoter mutant"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 3301  End: 3703 (Complementary)" 
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COMMENT                                   #71=(CLinePar 
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COMMENT                                        "origin plasmid" 1) 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                        "   complement(3301..3703)" 1))) 
COMMENT                                 "pMB1 ori") 
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COMMENT                                        "phage f1 origin" 1) 
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COMMENT                          "Replication Origin (2 total)") 
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COMMENT                                 "SV40 polyA early") 
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COMMENT                                        "   complement(5822..5975)" 1))) 
COMMENT                                 "synthetic polyA"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     misc_feature    32..672
                     /vntifkey="21"
                     /label=5'\FR\of\hIL6
                     /note="5' flanking region of the human interleukin 6 gene"
     promoter        673..1208
                     /vntifkey="29"
                     /label=hIL6\promoter\mutant
                     /note="mutated human interleukin 6 promoter"
     CDS             1256..2908
                     /vntifkey="4"
                     /label=LUCm
                     /note="mutated firefly luciferase coding sequence"
     terminator      2996..3092
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(2996..3092)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(3301..3703)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4244..5104)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      5236..5691
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     terminator      complement(5822..5975)
                     /vntifkey="43"
                     /label=synthetic\polyA
                     /note="synthetic polyA signal"
BASE COUNT     1585 a      1429 c      1432 g      1536 t 
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag 
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat 
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggac 
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga 
     1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 
     1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 
     1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 
     1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 
     1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 
     1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 
     1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 
     1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 
     1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 
     1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 
     1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 
     1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 
     1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 
     2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 
     2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 
     2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 
     2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 
     2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 
     2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 
     2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 
     2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 
     2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 
     2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 
     2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 
     2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 
     2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 
     2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 
     2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc 
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc 
     3181 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 
     3241 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 
     3301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
     3361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
     3421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
     3481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     3541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     3601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     3661 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc 
     3721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     3781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     3841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     3901 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     3961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     4021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     4081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     4141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     4201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     4261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     4321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     4381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     4441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     4501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     4561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     4621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     4681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     4741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     4801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     4861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     4921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     4981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     5041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     5101 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 
     5161 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 
     5221 gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 
     5281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 
     5341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 
     5401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 
     5461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 
     5521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 
     5581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 
     5641 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc 
     5701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 
     5761 agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg 
     5821 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt 
     5881 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt 
     5941 ccccagtgca agtgcaggtg ccagaacatt tctctatcga ta 
//
