ID pMRS101-R standard; circular DNA; ; 8752 BP. DE LMBP 4732 CC Accession number: LMBP 4732 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 4-JUN-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS 696..2304 FT /vntifkey="4" FT /label=streptomycin\resistance FT /note="selection marker for integration; last 10 codons of the original strB coding sequence are missing" FT CDS 2331..3191 FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 3732..4010 FT /vntifkey="33" FT /label=pMB1\ori FT /note="E. coli plasmid pMB1 origin" FT promoter 4016..4032 FT /vntifkey="30" FT /label=T3\promoter FT /note="phage T3 promoter" FT rep_origin 4139..4526 FT /vntifkey="33" FT /label=R6K\ori FT /note="E. coli plasmid R6K origin; defective pir gene" FT rep_origin complement(5243..5353) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT /note="broad host range plasmid RK2/RP4 origin of transfer" FT CDS 5767..6270 FT /vntifkey="4" FT /label=insB FT /note="E. coli insertion sequence IS1 insB" FT CDS complement(6408..7829) FT /vntifkey="4" FT /label=sacB FT /note="B. subtilis levansucrase coding sequence" SQ Sequence 8752 BP; 2091 A; 2104 C; 2219 G; 2338 t; gtcgacggat ccccgggtac cataagtaga agcagcaacc caagtagctt taccagcatc 60 cggttcaacc agcatagtaa gaatcttact ggacatcggc agttcttcga acagtgcgcc 120 aactaccagc tctttctgca gttcattcag ggcaccggag aacctgcgtg caatccatct 180 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat ccatggatta cgcgttaacc 240 cgggcccgcg gatgcatatg atcttaaggc ctaggtctag agtctttgtt ttgacgccat 300 tagcgtacgt aacaatcctc gttaaaggac aaggacctga gcggaagtgt atcgtacagt 360 agacggagta tactagtata gtctatagtc cgtggaatta ttatatttat ctccgacgat 420 attctcatca gtgaaatcca ggggaattct catgtttgac agcttatcat cgataagctt 480 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct 540 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg 600 gttatgccgg tactgccggg cctcttgcgg gataactgca cattcgggat atttctctat 660 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg 720 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc 780 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg 840 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca 900 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac 960 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac 1020 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa 1080 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg 1140 acgaggacaa gagtacgccg ctgcacgatc ttttggctcg tgtcgaacga gagctaccgg 1200 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga 1260 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg 1320 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg 1380 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag 1440 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat 1500 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga 1560 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa 1620 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg 1680 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga 1740 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac 1800 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc 1860 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca 1920 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag 1980 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc 2040 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc 2100 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat 2160 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga 2220 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca 2280 acgcgatcta gctatcgcgg ccgctctagc tagattgaaa aaggaagagt atgagtattc 2340 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 2400 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 2460 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 2520 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 2580 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 2640 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 2700 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 2760 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 2820 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 2880 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 2940 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 3000 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 3060 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 3120 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 3180 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 3240 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 3300 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 3360 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 3420 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 3480 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 3540 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 3600 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 3660 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 3720 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 3780 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 3840 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 3900 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 3960 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg cgcgcaatta 4020 accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc gatcaagcag 4080 gtgcgacaga cgtcatacta gatatctaga tccggcccga tgcgtccggc gtagaggatc 4140 tgaagatcag cagttcaacc tgttgatagt acgtactaag ctctcatgtt tcacgtacta 4200 agctctcatg tttaacgtac taagctctca tgtttaacga actaaaccct catggctaac 4260 gtactaagct ctcatggcta acgtactaag ctctcatgtt tcacgtacta agctctcatg 4320 tttgaacaat aaaattaata taaatcagca acttaaatag cctctaaggt tttaagtttt 4380 ataagaaaaa aaagaatata taaggctttt aaagctttta aggtttaacg gttgtggaca 4440 acaagccagg gatgtaacgc actgagaagc ccttagagcc tctcaaagca attttcagtg 4500 acacaggaac acttaacggc tgacatggga attctactgt ttgggtgtat gagctatttg 4560 caaaggaaat tggtgatgac aaagctcggc gctatttgaa gaaaatcgac tatggcaacg 4620 ccgatccttt ttgtccggtg ttgggttgaa ggtgaagccg gtcggggccg cagcgggggc 4680 cggcttttca gccttgcccc cctgcttcgg ccgccgtggc tccggcgtct tgggtgccgg 4740 cgcgggttcc gcagccttgg cctgcggtgc gggcacatcg gcgggcttgg ccttgatgtg 4800 ccgcctggcg tgcgagcgga acgtctcgta ggagaacttg accttccccg tttcccgcat 4860 gtgctcccaa atggtgacga gcgcatagcc ggacgctaac gccgcctcga catccgccct 4920 caccgccagg aacgcaaccg cagcctcatc acgccggcgc ttcttggccg cgcgggattc 4980 aacccactcg gccagctcgt cggtgtagct ctttggcatc gtctctcgcc tgtcccctca 5040 gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca aaacagcagg 5100 gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga ttttttcggt 5160 atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg tagactttcc 5220 ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac ccgcgagcgg 5280 gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga atcctgctct 5340 gcgaggctgg ccggctaccg ccggcgtaac agatgagggc aagcggatgg ctgatgaaac 5400 caagccaacc aggaagggca gcccacctat caaggtgtac tgccttccag acgaacgaag 5460 agcgattgag gaaaaggcgg cggcggccgg catgagcctg tcggcctacc tgctggccgt 5520 cggccagggc tacaaaatca cgggcgtcgt ggactatgag cacgtccgcg agctggcccg 5580 catcaatggc gacctgggcc gcctgggcgg cctgctgaaa ctctggctca ccgacgaccc 5640 gcgcacggcg cggttcggtg atgccacgat ccgggcaacg ttgttgccat tgctgcagtt 5700 cacttacacc gcttctcaac ccggtacgca ccataaaatc attgatatgg ccatgaatgg 5760 cgttggatgc cgggcaacag cccgcattat gggcgttggc ctcaacacga ttttacgtca 5820 cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc 5880 gtctgcgcgg aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg 5940 ctgttttacg cgtatgacag tctccggaag acggttgttg cgcacgtatt cggtgaacgc 6000 actatggcga cgctggggcg tcttatgagc ctgctgtcac cctttgacgt ggtgatatgg 6060 atgacggatg gctggccgct gtatgaatcc cgcctgaagg gaaagctgca cgtaatcagc 6120 aagcgatata cgcagcgaat tgagcggcat aacctgaatc tgaggcagca cctggcacgg 6180 ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg 6240 cattatctga acataaaaca ctatcaataa gttggagtca ttaccaaaag gttaggaata 6300 cggttagcca tttgcctgct tttatatagt tcatatggga ttcaccttta tgttgataag 6360 aaataaaaga aaatgccaat aggatatcgg cattttcttt tgcgttttta tttgttaact 6420 gttaattgtc cttgttcaag gatgctgtct ttgacaacag atgttttctt gcctttgatg 6480 ttcagcagga agcttggcgc aaacgttgat tgtttgtctg cgtagaatcc tctgtttgtc 6540 atatagcttg taatcacgac attgtttcct ttcgcttgag gtacagcgaa gtgtgagtaa 6600 gtaaaggtta catcgttagg atcaagatcc atttttaaca caaggccagt tttgttcagc 6660 ggcttgtatg ggccagttaa agaattagaa acataaccaa gcatgtaaat atcgttagac 6720 gtaatgccgt caatcgtcat ttttgatccg cgggagtcag tgaacaggta ccatttgccg 6780 ttcattttaa agacgttcgc gcgttcaatt tcatctgtta ctgtgttaga tgcaatcagc 6840 ggtttcatca cttttttcag tgtgtaatca tcgtttagct caatcatacc gagagcgccg 6900 tttgctaact cagccgtgcg ttttttatcg ctttgcagaa gtttttgact ttcttgacgg 6960 aagaatgatg tgcttttgcc atagtatgct ttgttaaata aagattcttc gccttggtag 7020 ccatcttcag ttccagtgtt tgcttcaaat actaagtatt tgtggccttt atcttctacg 7080 tagtgaggat ctctcagcgt atggttgtcg cctgagctgt agttgccttc atcgatgaac 7140 tgctgtacat tttgatacgt ttttccgtca ccgtcaaaga ttgatttata atcctctaca 7200 ccgttgatgt tcaaagagct gtctgatgct gatacgttaa cttgtgcagt tgtcagtgtt 7260 tgtttgccgt aatgtttacc ggagaaatca gtgtagaata aacggatttt tccgtcagat 7320 gtaaatgtgg ctgaacctga ccattcttgt gtttggtctt ttaggataga atcatttgca 7380 tcgaatttgt cgctgtcttt aaagacgcgg ccagcgtttt tccagctgtc aatagaagtt 7440 tcgccgactt tttgatagaa catgtaaatc gatgtgtcat ccgcattttt aggatctccg 7500 gctaatgcaa agacgatgtg gtagccgtga tagtttgcga cagtgccgtc agcgttttgt 7560 aatggccagc tgtcccaaac gtccaggcct tttgcagaag agatattttt aattgtggac 7620 gaatcgaatt caggaacttg atatttttca tttttttgct gttcagggat ttgcagcata 7680 tcatggcgtg taatatggga aatgccgtat gtttccttat atggcttttg gttcgtttct 7740 ttcgcaaacg cttgagttgc gcctcctgcc agcagtgcgg tagtaaaggt taatactgtt 7800 gcttgttttg caaacttttt gatgttcatc gttcatgtct ccttttttat gtactgtgtt 7860 agcggtctgc ttcttccagc cctcctgttt gaagatggca agttagttac gcacaataaa 7920 aaaagaccta aaatatgtaa ggggtgacgc caaagtatac actttgccct ttacacattt 7980 taggtcttgc ctgctttatc agtaacaaac ccgcgcgatt tacttttcga cctcattcta 8040 ttagactctc gtttggattg caactggtct attttcctct tttgtttgat agaaaatcat 8100 aaaaggattt gcagactacg ggcctaaaga actaaaaaat ctatctgttt cttttcattc 8160 tctgtatttt ttatagtttc tgttgcatgg gcataaagtt gcctttttaa tcacaattca 8220 gaaaatatca taatatctca tttcactaaa taatagtgaa cggcaggtat atgtgatggg 8280 ttaaaaagga tcttaaggcc taggtctagc ttggctgcag gggggggggg gggacttcca 8340 ttgttcattc cacggacaaa aacagagaaa ggaaacgaca gaggccaaaa agcctcgctt 8400 tcagcacctg tcgtttcctt tcttttcaga gggtatttta aataaaaaca ttaagttatg 8460 acgaagaaga acggaaacgc cttaaaccgg aaaattttca taaatagcga aaacccgcga 8520 ggtcgccgcc ccgtaacctg tcggatcacc ggaaaggacc cgtaaagtga taatgattat 8580 catctacata tcacaacgtg cgtggaggcc atcaaaccac gtcaaataat caattatgac 8640 gcaggtatcg tattaattga tctgcatcaa cttaacgtaa aaacaacttc agacaataca 8700 aatcagcgac actgaatacg gggcaacctc atgtcccccc ccccccctgc ag 8752 //