LOCUS pMahIL6P 4973 bp dsDNA Circular DEFINITION LMBP 4697. COMMENT This file is created by Vector NTI 4.0 from InforMax, Inc., USA: COMMENT (800)357-3114 or http://www.informaxinc.com/ COMMENT VNTREPLTYPE|Plasmid| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pMahIL6P" 0 4973 COMMENT (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList COMMENT #0=(CFragment 3 1 0 544825719 0 "" 0 "" "" "" "" "" "" "pMa58" "" 0 0 1 COMMENT 3764 1 3764 1 3764 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0)) COMMENT #1=(CFragment 3 1 0 1751348224 0 "" 0 "" "" "" "" "" "" "pBLHIL6CAT" "" COMMENT 0 0 1 1190 3765 4954 32 1217 4 "" "tcga" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "XhoI" "" 1 0 0 0 1 0 0 0 0 0)) COMMENT #2=(CFragment 3 1 0 7103744 0 "" 0 "" "" "" "" "" "" "pMa58" "" 0 0 1 COMMENT 19 4955 4973 3785 3803 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "HindIII" "" 1 0 0 0 1 0 0 0 0 0) COMMENT (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0))) (CObList)) COMMENT (CDocSetData 1 1 0 0 1 0 " all enzymes of DMB" 1 1 1 1 0 0 1 1 0 1 10 6 COMMENT 4000 50 0 1 0 (CHomObj 0 0 0 3 75) COMMENT (CWordArray 0 4 15 20 21 22 29 30 33 43) COMMENT (CWordArray 47 48 49 50 51 52 0 1 2 3 4 5 7 6 8 9 10 60 11 12 13 14 15 COMMENT 16 17 18 19 20 21 22 53 23 54 24 25 26 55 27 28 29 30 31 32 33 34 35 56 COMMENT 36 57 37 58 38 39 40 41 42 43 44 59 45 46) COMMENT (CStringList "AvaI" "BamHI" "EcoRI" "HindIII" "XbaI" "XhoI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList)) COMMENT (CMolPar 0 0 0 1 4973 1 4973 0 0 0 0 0 0 0 0) (CStringList) (CStringList) COMMENT (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList #3=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 3765 0) COMMENT #4=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 3755 0) COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 4955 0) COMMENT #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4963 0) COMMENT #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 0) COMMENT #8=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 3760 0)) COMMENT (CObList #9=(CFSignal (CObList) "CAMs" 4 0 1 2861 3520 1) COMMENT #10=(CFSignal (CObList) "AMP" 4 0 1 1686 2546 1) COMMENT #11=(CFSignal (CObList) "f1 ori" 33 0 0 313 768 1) COMMENT #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 772 1145 1) COMMENT #13=(CFSignal (CObList) "fd terminator" 43 0 0 24 105 1) COMMENT #14=(CFSignal (CObList) "fd terminator" 43 0 0 134 215 1) COMMENT #15=(CFSignal (CObList) "hIL6 promoter" 29 0 0 4406 4941 1)) (CObList) COMMENT (CObList) (CObList) (CObList) (CObList) (CObList) COMMENT (CTextView 0 COMMENT #16=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #17=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) COMMENT (CObjectList COMMENT #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA Plasmid pMahIL6P") COMMENT #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4697") COMMENT #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "length: 4973 bp") COMMENT #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "storage type: Constructed") COMMENT #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "form: Circular"))) COMMENT "General Description") COMMENT #23=(CFolderPar COMMENT (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #24=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #0# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 1 to 3764") COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "length: 3764") COMMENT #27=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 1 to 3764") COMMENT #28=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " Start of Molecule"))) "Left Terminus") COMMENT #30=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " BamHI site #1"))) "Right Terminus"))) COMMENT "#1: FRAGMENT of pMa58 ") COMMENT #32=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #33=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 32 to 1217 (+ fill of 4)") COMMENT #34=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "length: 1190") COMMENT #35=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 3765 to 4954") COMMENT #36=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #37=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " BamHI site #1"))) "Left Terminus") COMMENT #38=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #39=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " XhoI site #1") COMMENT #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " Completely filled in"))) "Right Terminus"))) COMMENT "#2: FRAGMENT of pBLHIL6CAT ") COMMENT #41=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (5 #2# 0) 1 2 1 0 195) COMMENT (CObjectList COMMENT #42=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "parent position: from 3785 to 3803") COMMENT #43=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) "length: 19") COMMENT #44=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) COMMENT "molecule position: from 4955 to 4973") COMMENT #45=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #46=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " HindIII site #1") COMMENT #47=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " Completely filled in"))) "Left Terminus") COMMENT #48=(CFolderPar COMMENT (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) COMMENT (CObjectList COMMENT #49=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) COMMENT " End of Molecule"))) "Right Terminus"))) COMMENT "#3: FRAGMENT of pMa58 "))) "Component Fragments") COMMENT #50=(CFolderPar COMMENT (CGroupPar (CParagraph 10 (6 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #51=(CFolderPar COMMENT 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#168=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -867 100) COMMENT (CObjectList))))) (CSeqView 10 6 (CObjectList)))) FEATURES Location/Qualifiers CDS complement(2861..3520) /note="chloramphenicol sensitive (mutated gene)" /label=CAMs CDS complement(1686..2546) /label=AMP rep_origin 313..768 /label=f1\ori rep_origin complement(772..1145) /label=pMB1\ori terminator 24..105 /label=fd\terminator terminator 134..215 /label=fd\terminator promoter 4406..4941 /label=hIL6\promoter ORIGIN 1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt 61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc 121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag 181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg 241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 301 cggatgcaga tcacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt 541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt 661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttga tctgcgctcg 781 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 841 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 901 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 961 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 1021 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 1081 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 1141 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 1201 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 1261 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 1321 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 1381 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 1441 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 1501 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 1561 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 1621 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 1681 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 1741 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 1801 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 1861 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 1921 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 1981 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 2041 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 2101 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 2161 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 2221 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 2281 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 2341 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 2401 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 2461 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 2521 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcagacag 2581 ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga 2641 cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag ttgactcccc gcgcgcgatg 2701 ggtcgaattt gctttcgaaa aaaaagcccg ctcattaggc gggctaaaaa aaagcccgct 2761 cattaggcgg gctcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc 2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg 2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg 3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctaa acggtctggt 3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cggggatcct cctgcaagag 3781 acaccatcct gagggaagag ggcttctgaa ccagcttgac ccaataagaa attcttgggt 3841 gccgacgcgg aagcagattc agagcctaga gccgtgcctg cgtccgtagt ttccttctag 3901 cttcttttga tttcaaatca agacttacag ggagagggag cgataaacac aaactctgca 3961 agatgccaca aggtcctcct ttgacatccc caacaaagag gtgagtagta ttctccccct 4021 ttctgccctg aaccaagtgg gcttcagtaa tttcagggct ccaggagacc tggggcccat 4081 gcaggtgccc cagtgaaaca gtggtgaaga gactcagtgg caatggggag agcactggca 4141 gcacaaggca aacctctggc acagagagca aagtcctcac tgggaggatt cccaaggggt 4201 cacttgggag agggcagggc agcagccaac ctcctctaag tgggctgaag caggtgaaga 4261 aagtggcaga agccacgcgg tggcaaaaag gagtcacaca ctccacctgg agacgccttg 4321 aagtaactgc acgaaatttg aggatggcca ggcagttcta caacagccgc tcacagggag 4381 agccagaaca cagaagaact cagatgactg gtagtattac cttcttcata atcccaggct 4441 tggggggctg cgatggagtc agaggaaact cagttcagaa catctttggt ttttacaaat 4501 acaaattaac tggaacgcta aattctagcc tgttaatctg gtcactgaaa aaaaattttt 4561 tttttttcaa aaaacatagc tttagcttat tttttttctc tttgtaaaac ttcgtgcatg 4621 acttcagctt tactctttgt caagacatgc caaagtgctg agtcactaat aaaagaaaaa 4681 aagaaagtaa aggaagagtg gttctgcttc ttagcgctag cctcaatgac gacctaagct 4741 gcacttttcc ccctagttgt gtcttgccat gctaaaggac gtcacattgc acaatcttaa 4801 taaggtttcc aatcagcccc acccgctctg gccccaccct caccctccaa caaagattta 4861 tcaaatgtgg gattttccca tgagtctcaa tattagagtc tcaaccccca ataaatatag 4921 gactggagat gtctgaggct cattctgccc tcgaagcttg gtctagaggt cga //