ID p50huIL6P-luc+ standard; circular DNA; ; 4919 BP. DT 23-OCT-2007 DE LMBP 4692. CC Accession number: LMBP 4692 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 29-APR-2013 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT promoter 88..137 FT /vntifkey="29" FT /label=hIL6\promoter\fragment FT /note="50 bp minimal human interleukin 6 promoter" FT CDS 185..1837 FT /vntifkey="4" FT /label=LUCm FT /note="mutated firefly luciferase coding sequence" FT terminator 1925..2021 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator complement(1925..2021) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT rep_origin complement(2238..2640) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3181..4041) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 4173..4628 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT terminator complement(4759..4912) FT /vntifkey="43" FT /label=synthetic\polyA FT /note="synthetic polyA signal" SQ Sequence 4919 BP; 1277 A; 1172 C; 1183 G; 1287 t; ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgc 60 atgcctgcag gtcgactcta gaggatcatt agagtctcaa cccccaataa atataggact 120 ggagatgtct gaggctcatt ctgccctcga agcttggcat tccggtactg ttggtaaagc 180 caccatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga 240 tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac 300 aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat 360 gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt 420 cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg 480 agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg 540 catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt 600 gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca 660 gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata 720 cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc 780 tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt 840 ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag 900 tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg 960 atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga 1020 ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac 1080 tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct 1140 ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg 1200 atatgggctc actgagacta catcagctat tctgattaca cccgaggggg atgataaacc 1260 gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg 1320 gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc 1380 cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca 1440 ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa 1500 gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct 1560 ccaacacccc aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga 1620 acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt 1680 ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt 1740 ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct 1800 cataaaggcc aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc 1860 gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 1920 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 1980 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 2040 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa 2100 ggatccgtcg atcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg 2160 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta 2220 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 2280 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 2340 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 2400 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 2460 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2520 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2580 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2640 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2700 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2760 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2820 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 2880 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2940 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 3000 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 3060 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 3120 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 3180 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 3240 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 3300 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 3360 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 3420 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3480 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3540 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3600 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3660 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 3720 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 3780 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 3840 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 3900 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 3960 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 4020 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 4080 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 4140 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc 4200 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 4260 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 4320 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 4380 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 4440 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 4500 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 4560 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt 4620 tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc 4680 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga 4740 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt 4800 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa 4860 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata 4919 //