LOCUS pBlueBacmsGCB1-H105F 6936 bp DNA circular 24-NOV-2015 DEFINITION LMBP 4643. SOURCE ORGANISM COMMENT Accession number: LMBP 4643 (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) Last modified: 24-NOV-2015 COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT ORIGDB|GenBank COMMENT VNTDBDATE|751369463| COMMENT LSOWNER| COMMENT VNTNAME|pBlueBacmsGCB1-H105F| COMMENT VNTAUTHORNAME|Demo User| COMMENT VNTOAUTHORNAME|UNKNOWN| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) COMMENT (SXF COMMENT (CGexDoc "pBlueBacmsGCB1-H105F" 0 6936 COMMENT (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) COMMENT (CObList COMMENT #0=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pBlueBachsGCA1His" "" 0 COMMENT 0 1 138 1 138 1 138 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) COMMENT #1=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "msGCB1-H105F" "" 0 0 1 COMMENT 1955 139 2093 2 1956 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 1 1 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) 1 0) COMMENT #2=(CFragment 3 1 0 2 0 "" 0 "" "" "" "" "" "" "pBlueBachsGCA1His" "" 0 COMMENT 0 1 4843 2094 6936 2274 7116 0 "" "" "" "" "" "" (CObList) COMMENT (CTerm 1 0 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) COMMENT (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") COMMENT (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 COMMENT 0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) COMMENT (CWordArray) (CStringList "BglII" "EcoRV" "HaeII" "HindIII" "SacI") COMMENT (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 COMMENT "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 COMMENT (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) COMMENT (CMolPar 0 0 0 0 4294967295 1 6936 0 0 0 0 0 0 0 0) (CStringList) COMMENT (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) COMMENT (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) COMMENT (CObList COMMENT #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 152 0 773 0 0 "") COMMENT #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 4 0 0 "") COMMENT #5=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2094 0 0 "") COMMENT #6=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 149 0 0 "") COMMENT #7=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 6 1424 0 2468 0 5162 0 COMMENT 5743 0 6180 0 6474 0 0 "")) COMMENT (CObList COMMENT #8=(CFSignal (CObList) "H105F" 62 0 0 506 508 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "506..508") COMMENT #9=(CFSignal (CObList) "mGucy1b3 mutant" 4 0 0 194 2053 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "194..2053") COMMENT #10=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2198 2272 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "2198..2272") COMMENT #11=(CFSignal (CObList) "V5 epitope" 22 0 0 2105 2146 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2105..2146") COMMENT #12=(CFSignal (CObList) "His-tag" 22 0 0 2156 2173 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "2156..2173") COMMENT #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2198 2272 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2198..2272)") COMMENT #14=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3726 4586 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "3726..4586") COMMENT #15=(CFSignal (CObList) "pMB1 ori" 33 0 0 5127 5521 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "5127..5521") COMMENT #16=(CFSignal (CObList) "lacZ fragment" 4 0 1 5522 6634 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "complement(5522..6634)") COMMENT #17=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6635 6909 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(6635..6909)") COMMENT #18=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) COMMENT (CStringList) 1 1 1 1 "7..95") COMMENT #19=(CFSignal (CObList) "AcNPV ORF 1629 fragment" 4 0 1 2460 3338 0 COMMENT (CStringList) (CStringList) 1 1 1 1 "complement(2460..3338)")) COMMENT (CObList) (CObList) (CObList) (CObList) COMMENT (CObList COMMENT #20=(CFSignal (CObList) "LMBP 4643" 2 0 0 0 0 0 (CStringList) COMMENT (CStringList) 1 1 0 0 "") COMMENT #21=(CFSignal (CObList) "Last modified: 24-NOV-2015" 2 0 0 0 0 0 COMMENT (CStringList) (CStringList) 1 1 0 0 "")) (CObList) COMMENT (CTextView 0 COMMENT #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) COMMENT (CObjectList COMMENT #23=(CRefLinePar COMMENT (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) COMMENT "pBlueBacmsGCB1-H105F" 2) 5 "" 0 4) COMMENT #24=(CFolderPar COMMENT (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) COMMENT (CObjectList COMMENT #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) COMMENT "DNA 'pBlueBacmsGCB1-H105F'" 1) COMMENT #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DMB 4643." 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31 0 0 400 0 0 0 0 3 2 1 18 COMMENT "Times New Roman") 1.74233 0.91 0 "LMBP 4643" COMMENT "LMBP 4643" 65535 -0.384028 -2.765 0 -10 7.0085 COMMENT 1.59371 Nil) COMMENT #278=(CLabel (CWidget 0 (15 #21# 0) 1 2 0 0 #152# 0 100) COMMENT (LOGPEN 0 0 0) 1 COMMENT (LOGFONT 55 20 0 0 400 0 0 0 0 3 2 1 34 "Arial") COMMENT 1.74233 0.611111 0 "Last modified: 24-NOV-2015" COMMENT "Last modified: 24-NOV-2015" 65535 -0.0576041 COMMENT -4.49312 0 -10 11.5976 1.09448 Nil)) (CObjectList)) COMMENT #279=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil 1952937076 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 COMMENT (CStringList) 0 0 COMMENT (CObList COMMENT #280=(CSampFrag "pBlueBacmsGCB1-H105F" 2694 2468 "HaeII" 5162 "HaeII" COMMENT (LOGPEN 0 0 0) 3.53786e-057 6.95336e-308) COMMENT #281=(CSampFrag "pBlueBacmsGCB1-H105F" 1886 6474 "HaeII" 1424 "HaeII" COMMENT (LOGPEN 0 0 0) 1.6976e-313 9.72715e-290) COMMENT #282=(CSampFrag "pBlueBacmsGCB1-H105F" 1044 1424 "HaeII" 2468 "HaeII" COMMENT (LOGPEN 0 0 0) 4.67082e-316 2.22735e-310) COMMENT #283=(CSampFrag "pBlueBacmsGCB1-H105F" 581 5162 "HaeII" 5743 "HaeII" COMMENT (LOGPEN 0 0 0) 6.12476e-062 1.72735e-077) COMMENT #284=(CSampFrag "pBlueBacmsGCB1-H105F" 437 5743 "HaeII" 6180 "HaeII" COMMENT (LOGPEN 0 0 0) 1.39804e-076 2.25173e-310) COMMENT #285=(CSampFrag "pBlueBacmsGCB1-H105F" 294 6180 "HaeII" 6474 "HaeII" COMMENT (LOGPEN 0 0 0) 1.6976e-313 9.71865e-290)))) FEATURES Location/Qualifiers mutation 506..508 /vntifkey="62" /label=H105F /note="WT: CAT" CDS 194..2053 /vntifkey="4" /label=mGucy1b3\mutant terminator 2198..2272 /vntifkey="43" /label=SV40\polyA\early misc_marker 2105..2146 /vntifkey="22" /label=V5\epitope misc_marker 2156..2173 /vntifkey="22" /label=His-tag terminator complement(2198..2272) /vntifkey="43" /label=SV40\polyA\late CDS complement(2460..3338) /vntifkey="4" /label=AcNPV\ORF\1629\fragment CDS 3726..4586 /vntifkey="4" /label=ampicillin\resistance rep_origin 5127..5521 /vntifkey="33" /label=pMB1\ori CDS complement(5522..6634) /vntifkey="4" /label=lacZ\fragment promoter complement(6635..6909) /vntifkey="29" /label=AcNPV-ETL\promoter promoter 7..95 /vntifkey="29" /label=AcNPV-PH\promoter BASE COUNT 1789 a 1656 c 1697 g 1794 t ORIGIN 1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 181 tgcccttgac accatgtacg gtttcgtgaa ccatgccctg gagctgctgg tgatccgcaa 241 ttatggtccc gaggtgtggg aagacatcaa aaaagaggca cagctggatg aagaaggcca 301 gtttcttgtc agaataatat acgatgattc caaaacatat gacctggtgg ctgctgcaag 361 caaagtcctc aacctcaatg ctggcgaaat cctgcagatg tttgggaaga tgtttttcgt 421 cttctgccag gagtctggct atgataccat cttgcgtgtc ctgggatcta atgtcagaga 481 gtttttgcag aacctcgatg ccctcttcga tcatctggcc accatttacc ccgggatgcg 541 cgcgccttcc ttcaggtgca ccgatgcgga gaaaggcaaa gggctcatcc tgcactacta 601 ctcggaaaga gaggggcttc aggacatcgt gatcgggatt atcaagactg ttgctcaaca 661 gatacacggc actgagatag acatgaaggt gattcagcaa agaaatgaag aatgtgatca 721 cacccagttt ttaattgaag aaaaggaatc aaaagaagag gatttttatg aagatctgga 781 taggtttgaa gagaatggta cccaggaatc acgtatcagc ccttacacct tctgcaaagc 841 atttcctttt cacatcatat ttgaccggaa cctagtggtc actcagtgtg gcaatgccat 901 ctacagagtg ctcccccagc tccagcctgg gaactgcagc cttctgtctg tcttctctct 961 ggtccgccct cacatcgaca tcagtttcca tgggattctt tcacacatca atacagtctt 1021 tgtactgaga agcaaggaag ggttgctgga tgtggagaaa cttgagtgtg aggatgaact 1081 gactggagca gagattagct gcttacgtct caaaggccaa atgatctact taccagaagc 1141 agatagcatc ctcttcctct gttcaccaag tgtgatgaac ctggacgacc taacaagaag 1201 aggcctgtat ctgagtgaca tccctctcca cgatgctacc cgagacctgg ttcttttggg 1261 agaacagttc cgggaggagt acaaactgac acaagagctg gaaatcctca ccgacaggct 1321 gcagctcaca ctgagagcct tggaggatga gaagaaaaag acagacacat tgctgtattc 1381 tgtccttcct ccatctgttg ccaatgagct gagacacaag cgcccagtgc ctgccaaaag 1441 atacgacaat gtgaccatcc tcttcagcgg cattgtgggc ttcaatgctt tctgtagcaa 1501 gcatgcatct ggagaagggg ccatgaagat tgtcaatctc ctcaacgatc tctacacccg 1561 atttgacaca ctgactgatt cacgaaaaaa cccatttgtt tacaaggtgg aaacagttgg 1621 tgacaagtat atgacagtga gtggcttgcc agaaccttgt atccaccatg cacggtccat 1681 ttgccacctg gctttagaca tgatggaaat tgctggtcaa gttcaagtag atggtgaatc 1741 tgttcagata acaatcggga tccataccgg ggaggtggtg acaggtgtga ttggacagcg 1801 gatgcctcgg tattgtctct ttgggaatac cgtcaacctc acaagcagga cagaaaccac 1861 aggagaaaag ggaaagataa atgtttccga atatacatac aggtgtctca tgtctccaga 1921 aaactcggat ccactgttcc atttggagca cagaggccca gtgtctatga agggcaagaa 1981 ggaaccaatg caagtctggt tcctatccag gaaaaataca ggcacggagg aaacaaatga 2041 ggaggatgaa aactgagtgt caggttgtgg aatctagagc aagggcaatt cgaagcttag 2101 gcctggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca 2161 tcaccatcac cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa 2221 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 2281 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa 2341 gaggaggaaa ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac 2401 gcacctctgt tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat 2461 taagcgctag attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat 2521 tcattaaatt tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg 2581 tctttatatc tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta 2641 gtttcaaaca agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc 2701 aacgccacaa aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt 2761 gttttgtttt gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt 2821 tcatcactgt cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca 2881 tatttaacat cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg 2941 tcgttagaag ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg 3001 tcggctaaca cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc 3061 gggcgttttt gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta 3121 gaaagcgatg gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt 3181 ggtggagctg atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc 3241 ggaggcggag gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc 3301 aacacagtcg gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa 3361 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 3421 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 3481 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 3541 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 3601 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 3661 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 3721 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 3781 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 3841 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 3901 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 3961 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 4021 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 4081 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 4141 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 4201 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 4261 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 4321 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 4381 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 4441 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 4501 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 4561 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 4621 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 4681 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 4741 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 4801 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 4861 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 4921 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 4981 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 5041 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 5101 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 5161 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 5221 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 5281 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 5341 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 5401 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 5461 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 5521 catcctgcac catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac 5581 ggttaacgcc tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc 5641 gcacctcgcg gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt 5701 gcagttcaac caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt 5761 cgacgttcag acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac 5821 cgccgaaagg cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta 5881 cccgtaggta gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc 5941 tgaaatcatc attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat 6001 gcagcaacga gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat 6061 aactgccgtc actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa 6121 atgcgctcag gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc 6181 cgttgcacca cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt 6241 cctgtagcca gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg 6301 tgggaacaaa cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat 6361 cgtaaccgtg catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc 6421 actccagcca gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc 6481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 6541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 6601 agtcacgacg ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct 6661 gctctttgat acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca 6721 tgtttgcaat cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg 6781 ctggacacca cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa 6841 tgcaaacttt ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag 6901 cgagtgcctt cgactttttc gtgtccagtg tggctt //