LOCUS       pBlueBacmsGCA1C239A-C244A        7173 bp    DNA     circular     23-NOV-2015
DEFINITION  LMBP 4642.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4642
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 23-NOV-2015
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|751308387|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBlueBacmsGCA1C239A-C244A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBlueBacmsGCA1C239A-C244A" 0 7173 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 1 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList 
COMMENT         #0=(CFragment 3 1 0 3487498 0 "" 0 "" "" "" "" "" "" 
COMMENT             "pBlueBachsGCA1His" "" 0 0 1 138 1 138 1 138 0 "" "" "" "" "" "" 
COMMENT             (CObList) (CTerm 4 0 0 "" "" 0 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 0 -1610612699 0 "" 0 "" "" "" "" "" "" 
COMMENT             "msGCA1-C239A-C244A" "" 0 0 1 2192 139 2330 2 2193 0 "" "" "" "" "" 
COMMENT             "" (CObList) (CTerm 1 0 0 "BamHI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) 1 0) 
COMMENT         #2=(CFragment 3 1 0 0 0 "" 0 "" "" "" "" "" "" "pBlueBachsGCA1His" "" 0 
COMMENT             0 1 4843 2331 7173 2274 7116 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "HindIII" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 4 1 0 "" "" 0 0 0 0 0 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "EcoRV" "SacI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7173 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #3=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 1762 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 4 0 1671 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SacI" "gagctc" 6 0 2 149 0 1889 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "mGucy1a3 mutant" 4 0 0 213 2288 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "213..2285") 
COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3963 4823 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3963..4823") 
COMMENT        #8=(CFSignal (CObList) "lacZ fragment" 4 0 1 5759 6871 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(5759..6871)") 
COMMENT        #9=(CFSignal (CObList) "His-tag" 21 0 0 2393 2410 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2393..2410") 
COMMENT        #10=(CFSignal (CObList) "AcNPV ORF 1629 fragment" 4 0 1 2697 3575 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2697..3575)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 0 5364 5758 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5364..5758") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2435 2509 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2435..2509") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2435 2509 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "V5 epitope" 22 0 0 2342 2383 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "AcNPV-PH promoter" 29 0 0 7 95 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #16=(CFSignal (CObList) "AcNPV-ETL promoter" 29 0 1 6872 7146 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #17=(CFSignal (CObList) "A239" 62 0 0 927 929 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "927..929") 
COMMENT        #18=(CFSignal (CObList) "A244" 62 0 0 942 944 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "942..944")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "Last modified: 23-NOV-2015" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "LMBP 4642" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                    "pBlueBacmsGCA1C239A-C244A" 2) 5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA 'pBlueBacmsGCA1C239A-C244A'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DMB 4642" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/23/15 04:46" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7173 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #34=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 23-NOV-2015") 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4642"))) "Annotations") 
COMMENT              #40=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "parent position: from 1 to 138" 1) 
COMMENT                            #43=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 138" 1) 
COMMENT                            #44=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 1 to 138" 1) 
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COMMENT                                        "  Start of Molecule" 1))) 
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COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  BamHI site #1" 1))) "Right Terminus"))) 
COMMENT                          "#1: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=1-138\">pBlueBachsGCA1His</a> ") 
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COMMENT                                 "parent position: from 2 to 2193" 1) 
COMMENT                            #51=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 2192" 1) 
COMMENT                            #52=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 139 to 2330" 1) 
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COMMENT                                        "  BamHI site #1" 1))) "Left Terminus") 
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COMMENT                                        "  HindIII site #1" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of <a href=\"vnti:dnarna/msGCA1-C239A-C244A?sel=2-2193\">msGCA1-C239A-C244A</a> ") 
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COMMENT                                 "parent position: from 2274 to 7116" 1) 
COMMENT                            #59=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "original length: 4843" 1) 
COMMENT                            #60=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 2331 to 7173" 1) 
COMMENT                            #61=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  HindIII site #1" 1))) "Left Terminus") 
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COMMENT                                 (CGroupPar (CParagraph 0 (0 0) 1 2 2 0 178) 
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COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "  End of Molecule" 1))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#3: FRAGMENT of <a href=\"vnti:dnarna/pBlueBachsGCA1His?sel=2274-7116\">pBlueBachsGCA1His</a> "))) 
COMMENT                   "Component Fragments") 
COMMENT              #65=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #66=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #67=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 213 (3 #6# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 213   End: 2288" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   213..2285" 1))) "mGucy1a3 mutant") 
COMMENT                            #71=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2697 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2697  End: 3575 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2697..3575)" 1))) 
COMMENT                                 "AcNPV ORF 1629 fragment") 
COMMENT                            #75=(CFolderPar 
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COMMENT                                        "Start: 3963  End: 4823" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3963..4823" 1))) 
COMMENT                                 "ampicillin resistance") 
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COMMENT                                  (CParagraph 5759 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5759  End: 6871 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5759..6871)" 1))) 
COMMENT                                 "lacZ fragment"))) "CDS (4 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2393  End: 2410" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2393..2410" 1))) "His-tag"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "Start: 2342  End: 2383" 1))) 
COMMENT                                 "V5 epitope"))) "Misc. Marker (1 total)") 
COMMENT                     #91=(CFolderPar 
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COMMENT                                        "Start: 7     End: 95  " 1))) 
COMMENT                                 "AcNPV-PH promoter") 
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COMMENT                                        1))) "AcNPV-ETL promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                         "   5364..5758" 1))) "pMB1 ori"))) 
COMMENT                          "Replication Origin (1 total)") 
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COMMENT                                     #105=(CLinePar 
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COMMENT                                   "SV40 polyA early") 
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COMMENT                                           "Start: 2435  End: 2509 (Complementary)" 
COMMENT                                           1))) "SV40 polyA late"))) 
COMMENT                           "Terminator (2 total)") 
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COMMENT                                     #111=(CLinePar 
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COMMENT                           "Mutation (2 total)"))) "Feature Map") 
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COMMENT                                            " N1: 1762 " 1))))) "BstXI: 1 site") 
COMMENT                            8 4 "CCANNNNNNTGG" "GGTNNNNNNACC") 
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COMMENT                                            " N1: 4 " 1) 
COMMENT                                      #126=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 2) 1 2 2 0 191) 
COMMENT                                            " N2: 1671 " 1))))) "EcoRV: 2 sites") 
COMMENT                            3 3 "GATATC" "CTATAG") 
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COMMENT                                            " N1: 149 " 1) 
COMMENT                                      #130=(CLinePar 
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COMMENT                                            " N2: 1889 " 1))))) "SacI: 2 sites") 
COMMENT                            5 1 "GAGCTC" "CTCGAG"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
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FEATURES             Location/Qualifiers
     CDS             213..2288
                     /vntifkey="4"
                     /label=mGucy1a3\mutant
     mutation        942..944
                     /vntifkey="62"
                     /label=A244
                     /note="WT: TGT"
     mutation        927..929
                     /vntifkey="62"
                     /label=A239
                     /note="WT: TGC"
     CDS             3963..4823
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(5759..6871)
                     /vntifkey="4"
                     /label=lacZ\fragment
     misc_feature    2393..2410
                     /vntifkey="21"
                     /label=His-tag
     CDS             complement(2697..3575)
                     /vntifkey="4"
                     /label=AcNPV\ORF\1629\fragment
     rep_origin      5364..5758
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      2435..2509
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(2435..2509)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     misc_marker     2342..2383
                     /vntifkey="22"
                     /label=V5\epitope
     promoter        7..95
                     /vntifkey="29"
                     /label=AcNPV-PH\promoter
     promoter        complement(6872..7146)
                     /vntifkey="29"
                     /label=AcNPV-ETL\promoter
BASE COUNT     1806 a      1758 c      1779 g      1830 t 
ORIGIN
        1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt 
       61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca 
      121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat 
      181 tgcccttgcc actgccaagc tccaggaaca ccatgttctg caggaagttc aaggatctca 
      241 agatcacagg ggagtgtcct ttctccttac tggcccctgg tcaggttcct aaggagccaa 
      301 cagaggaggt ggctggaggc tctgagggct gccaggctac tctgcccatc tgccagtact 
      361 ttcctgagaa gaatgcagaa gggagtctcc cccaaagaaa gacaagccgc aacagagtct 
      421 acctgcacac cctggcagag agtatttgca agctcatctt cccagagtgt gagcgactga 
      481 accttgcact tcagagaacc ttggcaaagc ataaaataga agaaaacagg aaatcttcag 
      541 aaaaagaaga ccttgaaaaa ataatcgcag aagaagcaat tgcagcaggt gccccagtgg 
      601 aggcgctcaa agactctctg ggcgaggagc tgttcaagat ctgctatgag gaagatgagc 
      661 acattttggg cgtggttggc ggcaccctga aggactttct aaatagcttc agcacgctcc 
      721 tcaagcagag cagccactgc caagaggcgg agaggcgggg acgactggaa gatgcctcca 
      781 tcttatgcct ggacaaggac caggactttc taaatgttta ctacttcttc ccgaagagaa 
      841 ccacagccct gcttctccct ggtatcatta aagcggctgc tcgcatactg tacgaaagcc 
      901 acgtggaggt gtccctgatg cctcccgcct tccgaagtga cgctaccgag tttgtgaacc 
      961 agccctattt gctctactcc gttcatgtga agagcaccaa gccgtccctg tccccaggca 
     1021 agccccagtc ctcgctagtg atccccgctt cgctcttctg caagactttc ccgttccatt 
     1081 tcatgctgga ccgagacctg gccatcctgc agctgggtaa cggcatcaga aggctggtga 
     1141 acaagaggga cttccaaggg aagcccaact ttgaagagtt ctttgaaatt ctaactccca 
     1201 aaatcaacca gacatttagt ggcatcatga caatgttgaa catgcagttt gtcatccggg 
     1261 tgaggagatg ggataactcg gtgaagaaat cgtcaagggt tatggatctc aaaggtcaaa 
     1321 tgatctacat cgttgaatcc agtgccatct tgttcttagg gtcaccatgt gtggacaggc 
     1381 tggaagattt cacaggacgg gggctctatc tgtccgacat cccaattcat aacgccctga 
     1441 gggatgttgt cttgataggg gagcaggcac gggctcaaga tggcctcaag aagaggttgg 
     1501 ggaagctgaa ggcaaccctg gagcatgccc accaagccct ggaggaagag aagaagagga 
     1561 cagtggatct gctgtgctct atcttcccct ctgaggttgc tcagcagctg tggcaaggac 
     1621 aaattgtgca agccaagaaa ttcagcgagg tcaccatgct tttctcagat atcgtagggt 
     1681 tcactgctat ctgctctcag tgttcacctc tgcaggtcat cacgatgctc aacgctctct 
     1741 acactcgctt tgaccagcag tgtggagagc tggatgtcta caaggtggag accatcgggg 
     1801 atgcatattg tgtggcaggt ggattgcaca gagagagtga cacccatgct gtccagatag 
     1861 cactgatggc cctgaagatg atggagctct ccaatgaggt catgtctccc cacggagaac 
     1921 ctatcaagat gcgaattgga ctacattctg gatcagtgtt tgctggagtt gtcggagtga 
     1981 agatgccccg gtattgcctg tttggaaaca atgtcactct ggctaacaaa tttgaatcct 
     2041 gcagtgtgcc tcggaaaatc aatgtcagcc ccaccacata caggttactc aaagactgtc 
     2101 ctggctttgt gttcaccccg agatcaaggg aggagcttcc accaaacttc cctagtgaca 
     2161 ttcctgggat ctgtcacttt ctggatgctt atcaccatca aggacctaat tccaaaccat 
     2221 ggttccagga taaagatgtg gaagatggaa acgccaactt cttaggcaaa gcgtcagggg 
     2281 tagattagtg agccacatgc tcttatgttt gatgcctaag ggcaattcga agcttaggcc 
     2341 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg gtcatcatca 
     2401 ccatcaccat tgacgtctct agcttggagt cgacaacttg tttattgcag cttataatgg 
     2461 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     2521 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat ctctcgactc tgctgaagag 
     2581 gaggaaattc tccttgaagt ttccctggtg ttcaaagtaa aggagtttgc accagacgca 
     2641 cctctgttca ctggtccggc gtattaaaac acgatacatt gttattagta catttattaa 
     2701 gcgctagatt ctgtgcgttg ttgatttaca gacaattgtt gtacgtattt taataattca 
     2761 ttaaatttat aatctttagg gtggtatgtt agagcgaaaa tcaaatgatt ttcagcgtct 
     2821 ttatatctga atttaaatat taaatcctca atagatttgt aaaataggtt tcgattagtt 
     2881 tcaaacaagg gttgtttttc cgaaccgatg gctggactat ctaatggatt ttcgctcaac 
     2941 gccacaaaac ttgccaaatc ttgtagcagc aatctagctt tgtcgatatt cgtttgtgtt 
     3001 ttgttttgta ataaaggttc gacgtcgttc aaaatattat gcgcttttgt atttctttca 
     3061 tcactgtcgt tagtgtacaa ttgactcgac gtaaacacgt taaataaagc ctggacatat 
     3121 ttaacatcgg gcgtgttagc tttattaggc cgattatcgt cgtcgtccca accctcgtcg 
     3181 ttagaagttg cttccgaaga cgattttgcc atagccacac gacgcctatt aattgtgtcg 
     3241 gctaacacgt ccgcgatcaa atttgtagtt gagctttttg gaattatttc tgattgcggg 
     3301 cgtttttggg cgggtttcaa tctaactgtg cccgatttta attcagacaa cacgttagaa 
     3361 agcgatggtg caggcggtgg taacatttca gacggcaaat ctactaatgg cggcggtggt 
     3421 ggagctgatg ataaatctac catcggtgga ggcgcaggcg gggctggcgg cggaggcgga 
     3481 ggcggaggtg gtggcggtga tgcagacggc ggtttaggct caaatgtctc tttaggcaac 
     3541 acagtcggca cctcaactat tgtactggtt tcgggcgatg gtgcactctc agtacaatct 
     3601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct 
     3661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct 
     3721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga 
     3781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 
     3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcat 
     5761 cctgcaccat cgtctgctca tccatgacct gaccatgcag aggatgatgc tcgtgacggt 
     5821 taacgcctcg aatcagcaac ggcttgccgt tcagcagcag cagaccattt tcaatccgca 
     5881 cctcgcggaa accgacatcg caggcttctg cttcaatcag cgtgccgtcg gcggtgtgca 
     5941 gttcaaccac cgcacgatag agattcggga tttcggcgct ccacagtttc gggttttcga 
     6001 cgttcagacg tagtgtgacg cgatcggcat aaccaccacg ctcatcgata atttcaccgc 
     6061 cgaaaggcgc ggtgccgctg gcgacctgcg tttcaccctg ccataaagaa actgttaccc 
     6121 gtaggtagtc acgcaactcg ccgcacatct gaacttcagc ctccagtaca gcgcggctga 
     6181 aatcatcatt aaagcgagtg gcaacatgga aatcgctgat ttgtgtagtc ggtttatgca 
     6241 gcaacgagac gtcacggaaa atgccgctca tccgccacat atcctgatct tccagataac 
     6301 tgccgtcact ccagcgcagc accatcaccg cgaggcggtt ttctccggcg cgtaaaaatg 
     6361 cgctcaggtc aaattcagac ggcaaacgac tgtcctggcc gtaaccgacc cagcgcccgt 
     6421 tgcaccacag atgaaacgcc gagttaacgc catcaaaaat aattcgcgtc tggccttcct 
     6481 gtagccagct ttcatcaaca ttaaatgtga gcgagtaaca acccgtcgga ttctccgtgg 
     6541 gaacaaacgg cggattgacc gtaatgggat aggtcacgtt ggtgtagatg ggcgcatcgt 
     6601 aaccgtgcat ctgccagttt gaggggacga cgacagtatc ggcctcagga agatcgcact 
     6661 ccagccagct ttccggcacc gcttctggtg ccggaaacca ggcaaagcgc cattcgccat 
     6721 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc 
     6781 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt 
     6841 cacgacgttg taaaacgacg ggatctatca tttttagcag tgattctaat tgcagctgct 
     6901 ctttgataca actaatttta cgacgacgat gcgagctttt attcaaccga gcgtgcatgt 
     6961 ttgcaatcgt gcaagcgtta tcaatttttc attatcgtat tgttgcacat caacaggctg 
     7021 gacaccacgt tgaactcgcc gcagttttgc ggcaagttgg acccgccgcg catccaatgc 
     7081 aaactttccg acattctgtt gcctacgaac gattgattct ttgtccattg atcgaagcga 
     7141 gtgccttcga ctttttcgtg tccagtgtgg ctt 
//
