LOCUS       pDEST-EGFP              6450 bp    DNA     circular      9-DEC-2010
DEFINITION  LMBP 4542.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4542
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 9-DEC-2010
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|460310294|
COMMENT     VNTDBDATE|573141153|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pDEST-EGFP|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTOAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDEST-EGFP" 0 6450 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "Alw44I" "Kpn2I" "NcoI" "PvuII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5331 5400 1 6450 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "Kpn2I" "tccgga" 2 0 1 1845 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 5 361 0 612 0 2150 0 4204 0 
COMMENT            4907 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuII" "cagctg" 4 0 3 1749 0 3973 0 4581 0 0 
COMMENT            "")) 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "1635..2294") 
COMMENT        #5=(CFSignal (CObList) "pMB1 ori" 33 0 0 6118 6444 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "6118..6444") 
COMMENT        #6=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4346 5140 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4346..5140") 
COMMENT        #7=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 0 1 568 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1..568") 
COMMENT        #8=(CFSignal (CObList) "EGFP" 4 0 0 613 1329 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "613..1329") 
COMMENT        #9=(CFSignal (CObList) "attR1" 0 0 1 1402 1526 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1402..1526)") 
COMMENT        #10=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1534 1586 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "1534..1586") 
COMMENT        #11=(CFSignal (CObList) "HSV-TK polyA" 43 0 0 5331 5400 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5331..5400") 
COMMENT        #12=(CFSignal (CObList) "SV40 ori" 33 0 0 4211 4286 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4211..4286") 
COMMENT        #13=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3973 4254 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3973..4254") 
COMMENT        #14=(CFSignal (CObList) "5' UTR of amp, incl. RBS" 32 0 0 3918 3952 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3918..3952") 
COMMENT        #15=(CFSignal (CObList) "ampicillin resistance promoter" 30 0 0 3822 
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COMMENT        #16=(CFSignal (CObList) "f1 ori" 33 0 1 3366 3821 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "ccdB" 4 0 0 2636 2941 0 (CStringList) 
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COMMENT        #18=(CFSignal (CObList) "attR2" 0 0 0 2982 3106 0 (CStringList) 
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COMMENT        #19=(CFSignal (CObList) "SV40 polyA late" 43 0 1 3216 3312 0 
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COMMENT        #20=(CFSignal (CObList) "SV40 polyA early" 43 0 0 3216 3312 0 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.lmbp@dmbr.UGent.be\">bccm.lmbp@dmbr.UGent.be</a>" 
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FEATURES             Location/Qualifiers
     promoter        1..568
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             613..1329
                     /vntifkey="4"
                     /label=EGFP
     attenuator      complement(1402..1526)
                     /vntifkey="0"
                     /label=attR1
     CDS             1635..2294
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2636..2941
                     /vntifkey="4"
                     /label=ccdB
     attenuator      2982..3106
                     /vntifkey="0"
                     /label=attR2
     rep_origin      complement(3366..3821)
                     /vntifkey="33"
                     /label=f1\ori
     terminator      3216..3312
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3216..3312)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        3973..4254
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      6118..6444
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     RBS             3918..3952
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     CDS             4346..5140
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     rep_origin      4211..4286
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        3822..3917
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     promoter        1534..1586
                     /vntifkey="30"
                     /label=lacUV5\promoter
     terminator      5331..5400
                     /vntifkey="43"
                     /label=HSV-TK\polyA
BASE COUNT     1623 a      1636 c      1650 g      1541 t 
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 
     1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattctg cagtcgacgg 
     1381 taccgcgggc ccgggatcat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat 
     1441 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 
     1501 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt 
     1561 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag 
     1621 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 
     1681 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 
     1741 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 
     1801 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 
     1861 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 
     1921 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 
     1981 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 
     2041 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 
     2101 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 
     2161 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct 
     2221 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 
     2281 agggcggggc gtaaacgcgt ggatccggct tactaaaagc cagataacag tatgcgtatt 
     2341 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 
     2401 aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 
     2461 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 
     2521 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 
     2581 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca 
     2641 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 
     2701 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 
     2761 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 
     2821 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 
     2881 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 
     2941 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 
     3001 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 
     3061 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggtgatg atccaccgga 
     3121 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 
     3181 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 
     3241 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 
     3301 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 
     3361 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 
     3421 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 
     3481 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 
     3541 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 
     3601 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 
     3661 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 
     3721 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 
     3781 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 
     3841 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 
     3901 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 
     3961 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     4021 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 
     4081 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 
     4141 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 
     4201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 
     4261 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 
     4321 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 
     4381 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 
     4441 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 
     4501 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 
     4561 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 
     4621 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 
     4681 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 
     4741 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 
     4801 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 
     4861 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 
     4921 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 
     4981 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 
     5041 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 
     5101 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 
     5161 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 
     5221 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 
     5281 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 
     5341 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 
     5401 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 
     5461 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 
     5521 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 
     5581 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 
     5641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 
     5701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 
     5761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 
     5821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 
     5881 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 
     5941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 
     6001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 
     6061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 
     6121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 
     6181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 
     6241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 
     6301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 
     6361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 
     6421 tctgtggata accgtattac cgccatgcat 
//
