LOCUS       pBKEF-NLScre            6131 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4477.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 4477
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|748444351|
COMMENT     VNTDBDATE|748445178|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBKEF-NLScre|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     CDS             complement(4819..5613)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      complement(4559..4628)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(5673..5748)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(5705..5986)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      7..462
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(3525..3841)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        483..1626
                     /vntifkey="29"
                     /label=hEF1a\promoter
     intron          679..1617
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        complement(6042..6)
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     terminator      2699..3265
                     /vntifkey="43"
                     /label=hGM-CSF\polyA
     CDS             1661..2689
                     /vntifkey="4"
                     /label=cre
     CDS             1643..1660
                     /vntifkey="4"
                     /label=SV40\large\T\NLS
BASE COUNT     1330 a      1610 c      1709 g      1464 t        18 others
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtnn ngcggccgcn 
      481 nngcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga 
      541 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 
      601 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 
      661 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg 
      721 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat 
      781 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct 
      841 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg 
      901 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga 
      961 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg 
     1021 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc 
     1081 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct 
     1141 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg 
     1201 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc 
     1261 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca 
     1321 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag 
     1381 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta 
     1441 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 
     1501 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 
     1561 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 
     1621 tcgtgannnt ctagccacca tgcccaagaa gaagaaggtg tccaatttac tgaccgtaca 
     1681 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat 
     1741 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt 
     1801 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc 
     1861 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag taaaaactat 
     1921 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag 
     1981 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg 
     2041 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact 
     2101 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta 
     2161 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac 
     2221 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg 
     2281 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc 
     2341 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc 
     2401 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac 
     2461 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg 
     2521 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga 
     2581 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct 
     2641 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattaga ctagannntg 
     2701 agggtcccca cctgggaccc ttgagagtat caggtctccc acgtgggaga caagaaatcc 
     2761 ctgtttaata tttaaacagc agtgttcccc atctgggtcc ttgcacccct cactctggcc 
     2821 tcagccgact gcacagcggc ccctgcatcc ccttggctgt gaggcccctg gacaagcaga 
     2881 ggtggccaga gctgggaggc atggccctgg ggtcccacga atttgctggg gaatctcgtt 
     2941 tttcttctta agacttttgg gacatggttt gactcccgaa catcaccgac gtgtctcctg 
     3001 tttttctggg tggcctcggg acacctgccc tgcccccacg agggtcagga ctgtgactct 
     3061 ttttagggcc aggcaggtgc ctggacattt gccttgctgg atggggactg gggatgtggg 
     3121 agggagcaga caggaggaat catgtcaggc ctgtgtgtga aaggaagctc cactgtcacc 
     3181 ctccacctct tcacccccca ctcaccagtg tcccctccac tgtcacattg taactgaact 
     3241 tcaggataat aaagtgtttg cctccnnnga attctttgga tccactagtg tcgacctgca 
     3301 ggcgcgcgag ctccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat 
     3361 caaggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaatatac 
     3421 gagccggaag tataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacagtaa 
     3481 ttgcggctag cggatctgac ggttcactaa accnnnatgc atggcggtta tccacagaat 
     3541 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 
     3601 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 
     3661 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 
     3721 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 
     3781 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 
     3841 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 
     3901 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 
     3961 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 
     4021 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 
     4081 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 
     4141 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 
     4201 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 
     4261 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 
     4321 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg 
     4381 cagggcctgc cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt 
     4441 ggggtgggga aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat 
     4501 cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt 
     4561 gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct 
     4621 tccgtgtttc agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc 
     4681 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga 
     4741 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt 
     4801 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg 
     4861 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag 
     4921 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag 
     4981 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca 
     5041 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc 
     5101 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag 
     5161 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg 
     5221 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt 
     5281 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag 
     5341 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt 
     5401 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc 
     5461 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga 
     5521 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg 
     5581 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg 
     5641 atcgatcttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct 
     5701 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg 
     5761 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta 
     5821 tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg 
     5881 actttccaca cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 
     5941 gggagcctgg ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc 
     6001 ctcaggactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 
     6061 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 
     6121 ccgaaaagtg c 
//
