LOCUS       pBKEF-Kozakcre          6114 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4476.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 4476

COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|635268962|
COMMENT     VNTDBDATE|635268962|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBKEF-Kozakcre|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
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FEATURES             Location/Qualifiers
     CDS             complement(4802..5596)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      complement(4542..4611)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(5656..5731)
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(5688..5969)
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      7..462
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(3508..3824)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1641..2672
                     /vntifkey="4"
                     /label=cre
     promoter        483..1626
                     /vntifkey="29"
                     /label=hEF1a\promoter
     intron          679..1617
                     /vntifkey="15"
                     /label=intron\hEF1a\promoter
     promoter        complement(6025..6)
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     terminator      2682..3248
                     /vntifkey="43"
                     /label=hGM-CSF\polyA
BASE COUNT     1322 a      1608 c      1703 g      1463 t        18 others
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta 
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg 
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttacgcgtnn ngcggccgcn 
      481 nngcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga 
      541 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 
      601 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 
      661 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg 
      721 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat 
      781 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct 
      841 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg 
      901 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga 
      961 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg 
     1021 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc 
     1081 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct 
     1141 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg 
     1201 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc 
     1261 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca 
     1321 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag 
     1381 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta 
     1441 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa 
     1501 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga 
     1561 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg 
     1621 tcgtgannnt ctagcccacc atgtccaatt tactgaccgt acaccaaaat ttgcctgcat 
     1681 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc 
     1741 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg 
     1801 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt 
     1861 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc 
     1921 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt 
     1981 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg 
     2041 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc 
     2101 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta 
     2161 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt 
     2221 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta 
     2281 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc 
     2341 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca 
     2401 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg 
     2461 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg 
     2521 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg 
     2581 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg 
     2641 caatggtgcg cctgctggaa gatggcgatt agactagann ntgagggtcc ccacctggga 
     2701 cccttgagag tatcaggtct cccacgtggg agacaagaaa tccctgttta atatttaaac 
     2761 agcagtgttc cccatctggg tccttgcacc cctcactctg gcctcagccg actgcacagc 
     2821 ggcccctgca tccccttggc tgtgaggccc ctggacaagc agaggtggcc agagctggga 
     2881 ggcatggccc tggggtccca cgaatttgct ggggaatctc gtttttcttc ttaagacttt 
     2941 tgggacatgg tttgactccc gaacatcacc gacgtgtctc ctgtttttct gggtggcctc 
     3001 gggacacctg ccctgccccc acgagggtca ggactgtgac tctttttagg gccaggcagg 
     3061 tgcctggaca tttgccttgc tggatgggga ctggggatgt gggagggagc agacaggagg 
     3121 aatcatgtca ggcctgtgtg tgaaaggaag ctccactgtc accctccacc tcttcacccc 
     3181 ccactcacca gtgtcccctc cactgtcaca ttgtaactga acttcaggat aataaagtgt 
     3241 ttgcctccnn ngaattcttt ggatccacta gtgtcgacct gcaggcgcgc gagctccagc 
     3301 ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcaaggtc atagctgttt 
     3361 cctgtgtgaa attgttatcc gctcacaatt ccacacaata tacgagccgg aagtataaag 
     3421 tgtaaagcct ggggtgccta atgagtgagc taactcacag taattgcggc tagcggatct 
     3481 gacggttcac taaaccnnna tgcatggcgg ttatccacag aatcagggga taacgcagga 
     3541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 
     3601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 
     3661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 
     3721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 
     3781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 
     3841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 
     3901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 
     3961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 
     4021 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca 
     4081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 
     4141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 
     4201 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 
     4261 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 
     4321 tttaaatcaa tctaaagtat atatgagtaa cctgaggcta tggcagggcc tgccgccccg 
     4381 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa 
     4441 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc 
     4501 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt attctgtctt 
     4561 tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt ttcagttagc 
     4621 ctccccctag ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag 
     4681 ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg 
     4741 aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg 
     4801 ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga 
     4861 taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac 
     4921 gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga 
     4981 atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca 
     5041 cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg 
     5101 cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag 
     5161 tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa 
     5221 gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt 
     5281 gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt 
     5341 cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc 
     5401 gcgctgcctc gtcttgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa 
     5461 ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt 
     5521 gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc 
     5581 catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag 
     5641 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc 
     5701 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg 
     5761 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg 
     5821 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt 
     5881 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt 
     5941 ccacacccta actgacacac attccacagc tggttctttc cgcctcagga ctcttccttt 
     6001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     6061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgc 
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