LOCUS       pPICZTS                 5475 bp    DNA     circular      1-FEB-2010
DEFINITION  LMBP 4420.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4420
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 1-FEB-2010
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|477057011|
COMMENT     VNTDBDATE|544810454|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPICZTS|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTOAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPICZTS" 0 5475 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BglII" "KpnI" "XbaI" "PmeI" "Alw44I") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5475 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "BglII" "agatct" 2 0 2 2 0 1842 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2860 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3154 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PmeI" "gtttaaac" 5 0 1 414 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 3 623 0 4391 0 5061 0 0 
COMMENT            "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "ppMF" 4 0 0 949 1191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "949..1191") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of AOX1" 21 0 0 773 937 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "773..937") 
COMMENT        #10=(CFSignal (CObList) "AOX1 promoter" 29 0 0 7 772 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7..772") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 4745 5025 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4745..5025)") 
COMMENT        #12=(CFSignal (CObList) "CYC1 terminator" 43 0 0 4465 4735 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4465..4735") 
COMMENT        #13=(CFSignal (CObList) "zeocin resistance" 4 0 0 4045 4419 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4045..4419") 
COMMENT        #14=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 3979 4044 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3979..4044") 
COMMENT        #15=(CFSignal (CObList) "TEF1 promoter" 29 0 0 3565 3970 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3565..3970") 
COMMENT        #16=(CFSignal (CObList) "AOX1 terminator" 43 0 0 3299 3558 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3299..3558") 
COMMENT        #17=(CFSignal (CObList) "His-tag" 22 0 0 3202 3219 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3202..3219") 
COMMENT        #18=(CFSignal (CObList) "Myc-tag" 22 0 0 3157 3186 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3157..3186") 
COMMENT        #19=(CFSignal (CObList) "mature TS" 4 0 0 1219 3150 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1219..3150")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 4420" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 1-FEB-2010" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "pPICZTS" 2) 5 "" 
COMMENT                   0 4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pPICZTS'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4420." 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: LMBP Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/17/08 11:50" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/01/10 04:14" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5475 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-JAN-2010" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #36=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP Plasmid Collection" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.lmbp@dmbr.UGent.be\">bccm.lmbp@dmbr.UGent.be</a>" 
COMMENT                          1))) "Original Author") 
COMMENT              #39=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #42=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4420") 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 1-FEB-2010"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 949 (3 #8# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 949   End: 1191" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae alpha-mating factor 1 prepro secretion signal sequence" 
COMMENT                                        1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   949..1191" 1))) "ppMF") 
COMMENT                            #52=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1219 (3 #19# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1219  End: 3150" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Trypanosoma cruzi trans-sialidase mature sequence" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1219..3150" 1))) "mature TS") 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4045 (3 #13# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4045  End: 4419" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4045..4419" 1))) "zeocin resistance"))) 
COMMENT                          "CDS (3 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 773 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 773   End: 937 " 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' UTR of the P. pastoris AOX1 gene" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   773..937" 1))) "5' UTR of AOX1"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #68=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #69=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3157 (3 #18# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3157  End: 3186" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Myc epitope" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3157..3186" 1))) "Myc-tag") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3202 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3202  End: 3219" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyhistidine (6 x His) tag" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3202..3219" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (2 total)") 
COMMENT                     #79=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #80=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7 (3 #10# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7     End: 772 " 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "P. pastoris alcohol oxidase 1 promoter" 
COMMENT                                        1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7..772" 1))) "AOX1 promoter") 
COMMENT                            #85=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3565 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3565  End: 3970" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae translation elongation factor 1alpha promoter" 
COMMENT                                        1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3565..3970" 1))) "TEF1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #90=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #91=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3979 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3979  End: 4044" 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "synthetic prokaryotic EM7 promoter" 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3979..4044" 1))) 
COMMENT                                 "synthetic EM7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
COMMENT                     #96=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4745 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #98=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4745  End: 5025 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(4745..5025)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
COMMENT                     #102=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #103=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 3299 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 3299  End: 3558" 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "P. pastoris alcohol oxidase 1 terminator" 
COMMENT                                           1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #107=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3299..3558" 1))) 
COMMENT                                   "AOX1 terminator") 
COMMENT                             #108=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4465 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 4465  End: 4735" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "S. cerevisiae iso-1-cytochrome C terminator" 
COMMENT                                           1) 
COMMENT                                     #111=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   4465..4735" 1))) 
COMMENT                                   "CYC1 terminator"))) "Terminator (2 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #113=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #114=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #115=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #116=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 2860 " 1))))) "KpnI: 1 site") 
COMMENT                            5 1 "GGTACC" "CCATGG") 
COMMENT                      #117=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #118=(CGroupPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #119=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3154 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
COMMENT                      #120=(CRSFolderPar 
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FEATURES             Location/Qualifiers
     CDS             949..1191
                     /vntifkey="4"
                     /label=ppMF
                     /note="S. cerevisiae alpha-mating factor 1 prepro secretion signal sequence"
     misc_feature    773..937
                     /vntifkey="21"
                     /label=5'\UTR\of\AOX1
                     /note="5' UTR of the P. pastoris AOX1 gene"
     promoter        7..772
                     /vntifkey="29"
                     /label=AOX1\promoter
                     /note="P. pastoris alcohol oxidase 1 promoter"
     rep_origin      complement(4745..5025)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     terminator      4465..4735
                     /vntifkey="43"
                     /label=CYC1\terminator
                     /note="S. cerevisiae iso-1-cytochrome C terminator"
     CDS             4045..4419
                     /vntifkey="4"
                     /label=zeocin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3979..4044
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
                     /note="synthetic prokaryotic EM7 promoter"
     promoter        3565..3970
                     /vntifkey="29"
                     /label=TEF1\promoter
                     /note="S. cerevisiae translation elongation factor 1alpha promoter"
     terminator      3299..3558
                     /vntifkey="43"
                     /label=AOX1\terminator
                     /note="P. pastoris alcohol oxidase 1 terminator"
     misc_marker     3202..3219
                     /vntifkey="22"
                     /label=His-tag
                     /note="polyhistidine (6 x His) tag"
     misc_marker     3157..3186
                     /vntifkey="22"
                     /label=Myc-tag
                     /note="Myc epitope"
     CDS             1219..3150
                     /vntifkey="4"
                     /label=mature\TS
                     /note="Trypanosoma cruzi trans-sialidase mature sequence"
BASE COUNT     1324 a      1327 c      1387 g      1437 t 
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 
     1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt 
     1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac 
     1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct 
     1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac 
     1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct 
     1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc 
     1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg 
     1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa 
     1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca 
     1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac 
     1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat 
     1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg 
     1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg 
     1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt 
     2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg 
     2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg 
     2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa 
     2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg 
     2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt 
     2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg 
     2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc 
     2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg 
     2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt 
     2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat 
     2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag 
     2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa 
     2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg 
     2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg 
     2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt 
     2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt 
     3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg 
     3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac 
     3121 atggacagca gcagcgacac gaaggaattg ggtctagaac aaaaactcat ctcagaagag 
     3181 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttgtag ccttagacat 
     3241 gactgttcct cagttcaagt tgggcactta cgagaagacc ggtcttgcta gattctaatc 
     3301 aagaggatgt cagaatgcca tttgcctgag agatgcaggc ttcatttttg atactttttt 
     3361 atttgtaacc tatatagtat aggatttttt ttgtcatttt gtttcttctc gtacgagctt 
     3421 gctcctgatc agcctatctc gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc 
     3481 attcgagttt gatgtttttc ttggtatttc ccactcctct tcagagtaca gaagattaag 
     3541 tgagaccttc gtttgtgcgg atcccccaca caccatagct tcaaaatgtt tctactcctt 
     3601 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa 
     3661 gcacagcata ctaaattttc cctctttctt cctctagggt gtcgttaatt acccgtacta 
     3721 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat 
     3781 aaaaattttt atcacgtttc tttttcttga aatttttttt tttagttttt ttctctttca 
     3841 gtgacctcca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca 
     3901 tttttcttgt tctattacaa ctttttttac ttcttgttca ttagaaagaa agcatagcaa 
     3961 tctaatctaa ggggcggtgt tgacaattaa tcatcggcat agtatatcgg catagtataa 
     4021 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc 
     4081 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac 
     4141 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg 
     4201 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac 
     4261 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg 
     4321 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc 
     4381 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg gcggcccacg 
     4441 ggtcccaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt aattagttat 
     4501 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag 
     4561 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt 
     4621 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat 
     4681 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctgga 
     4741 gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 
     4801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 
     4861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 
     4921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 
     4981 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 
     5041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 
     5101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 
     5161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 
     5221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 
     5281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 
     5341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 
     5401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 
     5461 tttggtcatg agatc 
//
