LOCUS       pGAL1PSiST-1            6640 bp    DNA     circular     28-AUG-2007
DEFINITION  LMBP 4177.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4177
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 28-AUG-2007
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|455883223|
COMMENT     VNTDBDATE|455906187|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pGAL1PSiST-1|
COMMENT     VNTAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGAL1PSiST-1" 0 6640 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 21 22 62 29 30 32 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "ClaI" "EcoRV" "HindII" "HindIII" "SfiI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaGAL1 promoter (1)" 
COMMENT             "AGATCTGATATTGACGAAGAGGAACCAATGTAACGTTACACTGAAGAAAACACACAATAAACGGGAAGAAACGGTGTAAAAGTGTGAAAATAATTTTTGAATATCATTTCCCTTGGTTTAATTCCAAACGAAACGTGTTTTTTTTAGAGAATGGGAATTCTTATTGGATGTCTAGATTGTTTGTTTACTCCAGACTGTGCACAAAAACGTTTGGATGGATGATCAGAAGATATTTTTAGGCTTAGCTCTAAATATAAGAAATGATGCTTGAAAAACCAGACAGAAATTGAGTTTCAAAAATTGGTAATGTGAGGTATTAGTCAACTAACCAAATAACAATGCAAACCGGTTGATACATTTCATTTTGAAAATAATGAAACTGGAATTGGATGACCAGCACACAAACACATAAAGTAATTATGGGAATTAGAAGCGAACATAGAGGAGTACTTGGCCACGAACAGAATACAAGTGGGAACACTATTTTCTCCATTGTTTTAGTTCTGTTTTTTTGTCAGCCTAGTTTTGTGCTATGTGTAAAAAATATTGCCAAGAAAAAAAGCTTGTTTTGTGGCCAGTGTCCGAAAAAAATTTTGGGGAATCTTCGGATTAATTTATGTTTTCATTCCATCGGGGAAAGTGGGGGGGAAAAAATTTTAAGCAGTTCACAAAACCTTCCAAAAAATATATGGACAAAGATGATTGTATTTTCCCGACACCAAAATCATAATTAATTATGAGAAAGTTAAATGTAACGTTACAATTTATGTTTATTTGAAGGTGAAAAGCGATTTATGATTTTTCCGAAATGAAAATTTTTTTTAGGTTTATTTTTTTTGTCGGGCAAAGAAAAACTGAACAAGGATTATTAAAATTTTTGGTGTTTGTTTGTGTCTGGAGAATTCATTCCTCTCTCATCTTCACACAATGTTTAGACATCTGACACGATTCATGATAGTTCGGTTTCCGGGGTTGGTGTTTAGTTTTCGTTTTTCTTTTT" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 1000" 0 (CStringList) 0) 
COMMENT         #1=(COligo "CaGAL1 promoter (2)" 
COMMENT             "TTTTGGAAAGAATGTTTTAGCTCATTGGTTTTCTTTCTTCATTCAATAGTTTTGAAAGAATTTGCCCACTTGTTATTACAATCATATAAAATTAAACTTTGATATAAAATAGAGTTTGAAAGTTTCCCAGATCCTTTTTGATTTCTTTGTAAATTTTTTTTTCTCCCACATATACACACATACAAACCGATTTTTATAAGAAAGAGTTATACC" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 213" 0 (CStringList) 0) 
COMMENT         #2=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #3=(COligo "GAL1 promoter" 
COMMENT             "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT             "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #4=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6640 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #5=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 2 57 0 5859 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 23 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 132 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 3411 0 4915 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 5153 0 5846 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "ClaI" "atcgat" 3 0 2 104 0 695 0 0 "")) 
COMMENT       (CObList 
COMMENT        #11=(CFSignal (CObList) "lac promoter" 30 0 1 446 529 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(446..529)") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 627 1035 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(627..1035)") 
COMMENT        #13=(CFSignal (CObList) "CaURA3" 4 0 0 3402 4214 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3402..4214") 
COMMENT        #14=(CFSignal (CObList) "CaGAL1 promoter" 29 0 0 4593 5805 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4593..5805") 
COMMENT        #15=(CFSignal (CObList) "hIFN\337" 4 0 0 5873 6436 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5873..6436") 
COMMENT        #16=(CFSignal (CObList) "CYC1 terminator" 43 0 0 113 383 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "113..383") 
COMMENT        #17=(CFSignal (CObList) "3' UTR of hIFN\337" 21 0 0 6437 6636 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6437..6636") 
COMMENT        #18=(CFSignal (CObList) "ampicillin" 4 0 1 1576 2436 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1576..2436)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "LMBP 4177" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 28-AUG-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
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COMMENT                          "DNA Plasmid 'pGAL1PSiST-1'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4177." 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 08/28/07 10:13" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 08/28/07 04:36" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6640 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 28-AUG-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4177") 
COMMENT                     #39=(CFolderPar 
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COMMENT                                 "" 1))) "Last modified: 28-AUG-2007"))) 
COMMENT                   "Annotations") 
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COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1576  End: 2436 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #45=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene" 1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1576..2436)" 1))) 
COMMENT                                 "ampicillin") 
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COMMENT                                   #49=(CLinePar 
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COMMENT                                        "Start: 3402  End: 4214" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "C. albicans uracil 3 coding sequence" 1) 
COMMENT                                   #51=(CLinePar 
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COMMENT                                   #52=(CLinePar 
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COMMENT                                        "   3402..4214" 1))) "CaURA3") 
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COMMENT                                        "Start: 5873  End: 6436" 1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "human IFN\337 gene (signal + mature sequence)" 
COMMENT                                        1) 
COMMENT                                   #56=(CLinePar 
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COMMENT                                   #57=(CLinePar 
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COMMENT                                        "   5873..6436" 1))) "hIFN\337"))) 
COMMENT                          "CDS (3 total)") 
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COMMENT                                        "Start: 6437  End: 6636" 1) 
COMMENT                                   #61=(CLinePar 
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COMMENT                                        "3' UTR of the hIFN\337 gene" 1) 
COMMENT                                   #62=(CLinePar 
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COMMENT                                   #63=(CLinePar 
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COMMENT                                 "3' UTR of hIFN\337"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "Start: 4593  End: 5805" 1) 
COMMENT                                   #67=(CLinePar 
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COMMENT                                        "C. albicans galactokinase 1 promoter" 1) 
COMMENT                                   #68=(CLinePar 
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COMMENT                                   #69=(CLinePar 
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COMMENT                                        "   4593..5805" 1))) "CaGAL1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 446   End: 529  (Complementary)" 
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COMMENT                                        "   complement(446..529)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "Start: 627   End: 1035 (Complementary)" 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #79=(CLinePar 
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COMMENT                                        "   complement(627..1035)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
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COMMENT                                        "Start: 113   End: 383 " 1) 
COMMENT                                   #84=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "S. cerevisiae iso-1-cytochrome C terminator" 
COMMENT                                        1) 
COMMENT                                   #85=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   113..383" 1))) "CYC1 terminator"))) 
COMMENT                          "Terminator (1 total)"))) "Feature Map") 
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COMMENT                                        " N1: 23 " 1))))) "BamHI: 1 site") 1 5 
COMMENT                          "GGATCC" "CCTAGG") 
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COMMENT                                        " N2: 695 " 1))))) "ClaI: 2 sites") 2 4 
COMMENT                          "ATCGAT" "TAGCTA") 
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COMMENT                                        " N1: 132 " 1))))) "EcoRV: 1 site") 3 3 
COMMENT                          "GATATC" "CTATAG") 
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COMMENT                                   #101=(CLinePar 
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COMMENT                                         " N2: 4915 " 1))))) "HindII: 2 sites") 
COMMENT                          3 3 "GTYRAC" "CARYTG") 
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COMMENT                                           " N1: 5153 " 1) 
COMMENT                                     #105=(CLinePar 
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COMMENT                                           " N2: 5846 " 1))))) 
COMMENT                            "HindIII: 2 sites") 1 5 "AAGCTT" "TTCGAA") 
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COMMENT                                           " N2: 5859 " 1))))) "SfiI: 2 sites") 
COMMENT                           8 5 "GGCCNNNNNGGCC" "CCGGNNNNNCCGG"))) 
COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        complement(446..529)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(627..1035)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             3402..4214
                     /vntifkey="4"
                     /label=CaURA3
                     /note="C. albicans uracil 3 coding sequence"
     promoter        4593..5805
                     /vntifkey="29"
                     /label=CaGAL1\promoter
                     /note="C. albicans galactokinase 1 promoter"
     CDS             5873..6436
                     /vntifkey="4"
                     /label=hIFNß
                     /note="human IFNß gene (signal + mature sequence)"
     terminator      113..383
                     /vntifkey="43"
                     /label=CYC1\terminator
                     /note="S. cerevisiae iso-1-cytochrome C terminator"
     misc_feature    6437..6636
                     /vntifkey="21"
                     /label=3'\UTR\of\hIFNß
                     /note="3' UTR of the hIFNß gene"
     CDS             complement(1576..2436)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene"
BASE COUNT     1935 a      1342 c      1466 g      1897 t 
ORIGIN
        1 gaggcctagc ggccgcctag aggatccccg ggcgctaggc ggccgctagg cctttttggc 
       61 caagctcgaa tttcgaggaa ttcgagctcg gtacccgggg gatcgatccg tccccctttt 
      121 cctttgtcga tatcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat 
      181 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt 
      241 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt 
      301 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac 
      361 gctcgaaggc tttaatttgc aagctagctt ggcgtaatca tggtcatagc tgtttcctgt 
      421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
      481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
      541 tttccagtcg ggaaacctgt cgtgccagag atctctgcat taatgaatcg gccaacgcgc 
      601 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 
      661 ctcggtcgtt cggctgcggc gagcggtatc agatcgatct cactcaaagg cggtaatacg 
      721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 
      781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 
      841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 
      901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 
      961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 
     1021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 
     1081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 
     1141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 
     1201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 
     1261 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 
     1321 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 
     1381 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 
     1441 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 
     1501 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 
     1561 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 
     1621 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 
     1681 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 
     1741 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 
     1801 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 
     1861 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 
     1921 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 
     1981 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 
     2041 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 
     2101 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 
     2161 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 
     2221 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 
     2281 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 
     2341 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 
     2401 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 
     2461 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 
     2521 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 
     2581 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 
     2641 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 
     2701 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 
     2761 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 
     2821 tatcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 
     2881 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 
     2941 ccggccacgg ggcctgccac catacccacg ccgaaacaag cactaatagg aattgatttg 
     3001 gatggtataa acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt 
     3061 atttgatttt atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt 
     3121 gctgtagtgc cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag 
     3181 tatccaaaaa aacggctatt tttttgcaat cttatttcct gcatattata cagataacat 
     3241 aatgaaagaa aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac 
     3301 attgatcaat tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca 
     3361 gttgaagaaa gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga 
     3421 cctatagtga gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa 
     3481 tggaactgaa gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggagttcc 
     3541 tcgagttaat tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa 
     3601 tcaatgattt ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc 
     3661 aatttatgat ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat 
     3721 atattggtgg agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca 
     3781 ctgggaatgg agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag 
     3841 agccaagagg gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag 
     3901 aatattctca aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta 
     3961 ttgcccaacg tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg 
     4021 gagttggatt agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag 
     4081 ttgttagcac tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag 
     4141 atccagatat tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga 
     4201 ctggccaatt ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag 
     4261 aataaataaa taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta 
     4321 caatcaaagg tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt 
     4381 aaaaatggaa tcaataaagc cctgcgctca tgagcccgaa gtggcgagcc cgatcttccc 
     4441 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg cagcgcgcag 
     4501 ggtcagcctg aatacgcgtt taatgaccag cacagtcgtg atggcaaggt cagaatagcc 
     4561 caagtcggcc gaggggcctg tacagtgagg gaagatctga tattgacgaa gaggaaccaa 
     4621 tgtaacgtta cactgaagaa aacacacaat aaacgggaag aaacggtgta aaagtgtgaa 
     4681 aataattttt gaatatcatt tcccttggtt taattccaaa cgaaacgtgt tttttttaga 
     4741 gaatgggaat tcttattgga tgtctagatt gtttgtttac tccagactgt gcacaaaaac 
     4801 gtttggatgg atgatcagaa gatattttta ggcttagctc taaatataag aaatgatgct 
     4861 tgaaaaacca gacagaaatt gagtttcaaa aattggtaat gtgaggtatt agtcaactaa 
     4921 ccaaataaca atgcaaaccg gttgatacat ttcattttga aaataatgaa actggaattg 
     4981 gatgaccagc acacaaacac ataaagtaat tatgggaatt agaagcgaac atagaggagt 
     5041 acttggccac gaacagaata caagtgggaa cactattttc tccattgttt tagttctgtt 
     5101 tttttgtcag cctagttttg tgctatgtgt aaaaaatatt gccaagaaaa aaagcttgtt 
     5161 ttgtggccag tgtccgaaaa aaattttggg gaatcttcgg attaatttat gttttcattc 
     5221 catcggggaa agtggggggg aaaaaatttt aagcagttca caaaaccttc caaaaaatat 
     5281 atggacaaag atgattgtat tttcccgaca ccaaaatcat aattaattat gagaaagtta 
     5341 aatgtaacgt tacaatttat gtttatttga aggtgaaaag cgatttatga tttttccgaa 
     5401 atgaaaattt tttttaggtt tatttttttt gtcgggcaaa gaaaaactga acaaggatta 
     5461 ttaaaatttt tggtgtttgt ttgtgtctgg agaattcatt cctctctcat cttcacacaa 
     5521 tgtttagaca tctgacacga ttcatgatag ttcggtttcc ggggttggtg tttagttttc 
     5581 gtttttcttt ttttttggaa agaatgtttt agctcattgg ttttctttct tcattcaata 
     5641 gttttgaaag aatttgccca cttgttatta caatcatata aaattaaact ttgatataaa 
     5701 atagagtttg aaagtttccc agatcctttt tgatttcttt gtaaattttt ttttctccca 
     5761 catatacaca catacaaacc gatttttata agaaagagtt ataccctgca gctcgacctc 
     5821 gactgtttaa acctgcaggc atgcaagctt ggccaaaaag gcctcgagga acatgaccaa 
     5881 caagtgtctc ctccaaattg ctctcctgtt gtgcttctcc actacagctc tttccatgag 
     5941 ctacaacttg cttggattcc tacaaagaag cagcaatttt cagtgtcaga agctcctgtg 
     6001 gcaattgaat gggaggcttg aatactgcct caaggacagg atgaactttg acatccctga 
     6061 ggagattaag cagctgcagc agttccagaa ggaggacgcc gcattgacca tctatgagat 
     6121 gctccagaac atctttgcta ttttcagaca agattcatct agcactggct ggaatgagac 
     6181 tattgttgag aacctcctgg ctaatgtcta tcatcagata aaccatctga agacagtcct 
     6241 ggaagaaaaa ctggagaaag aagatttcac caggggaaaa ctcatgagca gtctgcacct 
     6301 gaaaagatat tatgggagga ttctgcatta cctgaaggcc aaggagtaca gtcactgtgc 
     6361 ctggaccata gtcagagtgg aaatcctaag gaacttttac ttcattaaca gacttacagg 
     6421 ttacctccga aactgaagat ctcctagcct gtgcctctgg gactggacaa ttgcttcaag 
     6481 cattcttcaa ccagcagatg ctgtttaagt gactgatggc taatgtactg catatgaaag 
     6541 gacactagaa gattttgaaa tttttattaa attatgagtt atttttattt atttaaattt 
     6601 tattttggaa aataaattat ttttggtgca aaagtccctc 
//
