LOCUS       pPIC9-TSE              10878 bp    DNA     circular     17-DEC-2012
DEFINITION  LMBP 4166.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4166
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 17-DEC-2012
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|645723326|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPIC9-TSE|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CObList) -1 "") 
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COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
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COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
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COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 2664 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 5 279 0 1775 0 2340 0 
COMMENT            5444 0 9902 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "Eco47III" "agcgct" 4 0 2 4654 0 7569 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 6488 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "773..937") 
COMMENT        #5=(CFSignal (CObList) "ppMF" 4 0 0 949 1191 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "949..1191") 
COMMENT        #6=(CFSignal (CObList) "E-tag" 22 0 0 3151 3189 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3151..3189") 
COMMENT        #7=(CFSignal (CObList) "AOX1 terminator" 43 0 0 4176 4435 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4176..4435") 
COMMENT        #8=(CFSignal (CObList) "Pichia HIS4" 4 0 1 4838 7369 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4838..7369)") 
COMMENT        #9=(CFSignal (CObList) "3' flanking region of AOX1" 21 0 0 7724 8481 0 
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COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 8765 9167 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "ampicillin resistance" 4 0 1 9708 10568 0 
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COMMENT        #12=(CFSignal (CObList) "AOX1 promoter" 29 0 0 7 772 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7..772") 
COMMENT        #13=(CFSignal (CObList) "mature TS" 4 0 0 1219 3141 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "fd g3 fragment" 4 0 0 3199 4090 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pPIC9-TSE'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: LMBP Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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FEATURES             Location/Qualifiers
     misc_feature    773..937
                     /vntifkey="21"
                     /label=5'\UTR\of\AOX1
     CDS             949..1191
                     /vntifkey="4"
                     /label=ppMF
                     /note="S. cerevisiae alpha mating factor 1 prepro sequence"
     misc_marker     3151..3189
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag; C-terminal"
     terminator      4176..4435
                     /vntifkey="43"
                     /label=AOX1\terminator
     CDS             complement(4838..7369)
                     /vntifkey="4"
                     /label=Pichia\HIS4
     misc_feature    7724..8481
                     /vntifkey="21"
                     /label=3'\flanking\region\of\AOX1
     rep_origin      complement(8765..9167)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(9708..10568)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        7..772
                     /vntifkey="29"
                     /label=AOX1\promoter
     CDS             1219..3141
                     /vntifkey="4"
                     /label=mature\TS
                     /note="Trypanosoma cruzi trans-sialidase gene; mature sequence"
     CDS             3199..4090
                     /vntifkey="4"
                     /label=fd\g3\fragment
                     /note="fragment of phage fd gene 3"
BASE COUNT     2788 a      2568 c      2663 g      2859 t 
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 
     1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt 
     1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac 
     1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct 
     1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac 
     1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct 
     1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc 
     1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg 
     1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa 
     1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca 
     1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac 
     1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat 
     1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg 
     1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg 
     1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt 
     2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg 
     2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg 
     2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa 
     2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg 
     2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt 
     2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg 
     2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc 
     2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg 
     2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt 
     2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat 
     2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag 
     2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa 
     2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg 
     2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg 
     2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt 
     2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt 
     3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg 
     3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac 
     3121 atggacagca gcagcgacac gaaggaattg ggtgcgccgg tgccgtatcc ggatccgctg 
     3181 gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa aacctcatac agaaaattca 
     3241 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt 
     3301 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca 
     3361 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt 
     3421 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct 
     3481 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa 
     3541 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt 
     3601 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact 
     3661 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg 
     3721 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag 
     3781 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat 
     3841 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt 
     3901 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt 
     3961 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat 
     4021 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt 
     4081 gctgctatcg ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg 
     4141 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt 
     4201 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg 
     4261 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca 
     4321 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg 
     4381 gtatttccca ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct 
     4441 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc 
     4501 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 
     4561 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 
     4621 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 
     4681 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 
     4741 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta 
     4801 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac 
     4861 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg 
     4921 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga 
     4981 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt 
     5041 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc 
     5101 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt 
     5161 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg 
     5221 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt 
     5281 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca 
     5341 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat 
     5401 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac 
     5461 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg 
     5521 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg 
     5581 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga 
     5641 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac 
     5701 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac 
     5761 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag 
     5821 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat 
     5881 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga 
     5941 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta 
     6001 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa 
     6061 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct 
     6121 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg 
     6181 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga 
     6241 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg 
     6301 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg 
     6361 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt 
     6421 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg 
     6481 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac 
     6541 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt 
     6601 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac 
     6661 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg 
     6721 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat 
     6781 cggggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat 
     6841 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag 
     6901 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc 
     6961 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga 
     7021 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag 
     7081 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat 
     7141 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg 
     7201 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca 
     7261 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat 
     7321 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg 
     7381 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc 
     7441 atcaggagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg 
     7501 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc 
     7561 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg 
     7621 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac 
     7681 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg 
     7741 gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat 
     7801 gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat 
     7861 cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga 
     7921 cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc 
     7981 gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag 
     8041 cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc 
     8101 caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag 
     8161 ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt 
     8221 gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc 
     8281 caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct 
     8341 gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat 
     8401 cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac 
     8461 gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg 
     8521 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 
     8581 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc 
     8641 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg 
     8701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 
     8761 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     8821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     8881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     8941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     9001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     9061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     9121 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     9181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     9241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     9301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     9361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     9421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     9481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     9541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     9601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     9661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     9721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     9781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     9841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     9901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     9961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
    10021 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
    10081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
    10141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
    10201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
    10261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
    10321 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
    10381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
    10441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
    10501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
    10561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
    10621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
    10681 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 
    10741 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta 
    10801 tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa 
    10861 aataacagtt attattcg 
//
