ID pPHT standard; circular DNA; ; 7417 BP. DT 24-OCT-2008 DE LMBP 3960 CC Accession number: LMBP 3960 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 28-OCT-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator complement(402..853) FT /vntifkey="43" FT /label=3'\UTR\of\mPGK1,\incl.\polyA FT CDS complement(1463..2593) FT /vntifkey="4" FT /label=HSV-TK FT /note="Herpes simplex thymidine kinase coding sequence" FT promoter complement(2691..3198) FT /vntifkey="29" FT /label=mPGK1\promoter FT /note="mouse phosphoglycerate kinase 1 promoter" FT terminator complement(3236..3514) FT /vntifkey="43" FT /label=3'\UTR\of\mPGK1,\incl.\polyA FT CDS complement(3518..4543) FT /vntifkey="4" FT /label=hygromycin\resistance FT promoter complement(4553..5060) FT /vntifkey="29" FT /label=mPGK1\promoter FT /note="mouse phosphoglycerate kinase 1 promoter" FT promoter complement(5170..5186) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(5250..5333) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(5425..5816) FT /vntifkey="33" FT /label=ColE1\ori FT CDS complement(6357..7217) FT /vntifkey="4" FT /label=ampicillin\resistance FT terminator complement(1393..1462) FT /vntifkey="43" FT /label=HSV-TK\polyA SQ Sequence 7417 BP; 1688 A; 2049 C; 2072 G; 1608 t; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac 420 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt 480 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca 540 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat 600 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta 660 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca 720 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt 780 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga 840 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata 900 caaaacaaaa gcgctcctcg taccagcgaa gaaggggcag agatgccgta gtcaggttta 960 gttcgtccgg cggcgccaga aatccgcgcg gtggtttttg ggggtcgggg gtgtttggca 1020 gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc aggccatcca 1080 aaaaccatgg gtctgtctgc tcagtccagt cgtggacctg accccacgca acgcccaaaa 1140 gaataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct aacccacggg 1200 gcccgtggct atggcgggct tgccgccccg acgttggctg cgagccctgg gccttcaccc 1260 gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt 1320 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac 1380 gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc 1440 cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgggca aacgtgcgcg 1500 ccaggtcgca gatcgtcggt atggagcctg gggtggtgac gtgggtctgg accatcccgg 1560 aggtaagttg cagcagggcg tcccggcagc cggcgggcga ttggtcgtaa tccaggataa 1620 agacgtgcat gggacggagg cgtttggcca agacgtccaa ggcccaggca aacacgttat 1680 acaggtcgcc gttgggggcc agcaactcgg gggcccgaaa cagggtaaat aacgtgtccc 1740 cgatatgggg tcgtgggccc gcgttgctct ggggctcggc accctggggc ggcacggccg 1800 tccccgaaag ctgtccccaa tcctcccacc acgacccgcc gccctgcaga taccgcaccg 1860 tattggcaag cagcccgtaa acgcggcgaa tcgcggccag catagccagg tcaagccgct 1920 cgccggggcg ctggcgtttg gccaggcggt cgatgtgtct gtcctccgga agggccccca 1980 acacgatgtt tgtgccgggc aaggtcggcg ggatgagggc cacgaacgcc agcacggcct 2040 ggggggtcat gctgcccata aggtatcgcg cggccgggta gcacaggagg gcggcgatgg 2100 gatggcggtc gaagatgagg gtgagggccg ggggcggggc atttgagctc ccagcctccc 2160 ccccgatatg aggagccaga acggcgtcgg tcacggcata aggcatgccc attgttatct 2220 gggcgcttgt cattaccacc gccgcgtccc cggccgatat ctcaccctgg tcgaggcggt 2280 gttgtgtggt gtagatgttc gcgattgtct cggaagcccc cagcacctgc cagtaagtca 2340 tcggctcggg tacgtagacg atatcgtcgc gcgaacccag ggccaccagc agttgcgtgg 2400 tggtggtttt ccccatcccg tgaggaccgt ctatataaac ccgcagtagc gtgggcattt 2460 tctgctccag gcggacttcc gtggcttctt gctgccggcg agggcgcaac gccgtacgtc 2520 ggttgctatg gccgcgagaa cgcgcagcct ggtcgaacgc agacgcgtgt tgatggcagg 2580 ggtacgaagc catacgcgct tctacaaggc gcttgccaaa gaggtgcggg agtttcacgc 2640 caccaagatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg tcgaaaggcc 2700 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc 2760 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc 2820 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg 2880 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag 2940 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg 3000 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg 3060 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta 3120 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc 3180 ctcccctacc cggtagaatt cgagctcggt acccggggat cctctagaac tagtggatct 3240 atagatcatg agtgggagga atgagctggc ccttaatttg gttttgcttg tttaaattat 3300 gatatccaac tatgaaacat tatcataaag caatagtaaa gagccttcag taaagagcag 3360 gcatttatct aatcccaccc cacccccacc cccgtagctc caatccttcc attcaaaatg 3420 taggtactct gttctcaccc ttcttaacaa agtatgacag gaaaaacttc cattttagtg 3480 gacatcttta ttgtttaata gatcatcaat ttctcgacta ttcctttgcc ctcggacgag 3540 tgctggggcg tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg 3600 ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg 3660 cgtcgcatcg accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat 3720 agagttggtc aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct 3780 ccggatgcct ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc 3840 agaagaagat gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa 3900 tgaccgctgt tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca 3960 cgaggtgccg gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg 4020 cgacggacgc actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag 4080 caatcgcgca tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg 4140 ggccgaaccc gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga 4200 ccggctgcag aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg 4260 cacggcggga gatgcaatag gtcaggctct cgctgaattc cccaatgtca agcacttccg 4320 gaatcgggag cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat 4380 cggcgcagct atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac 4440 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca 4500 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catggtggcg ggtcgaaagg 4560 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat 4620 gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc cctgagcccg 4680 cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca aagctgctat 4740 tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc catctgcacg 4800 agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag ctgcggggcg 4860 ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc accaaagaac 4920 ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag gccacttgtg 4980 tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct tgggaaaagc 5040 gcctccccta cccggtagaa ttcatcgatg atatcagatc ccccgggctg caggaattcg 5100 atatcaagct tatcgatacc gtcgacctcg agcggccgcc accgcggtgg agctccagct 5160 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc 5220 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 5280 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 5340 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 5400 ggagaggcgg tttgcgtatt gggcgctttt ccgcttcctc gctcactgac tcgctacgct 5460 cggtcgttcg actgcggcga gcggtactga ctcacacaaa aacggtaaca cagttatcca 5520 cagaatcagg ggataaggcc ggaaagaaca tgtgagcaaa agaccaggaa caggaagaag 5580 gccacgtagc aggcgttttt ccataggctc cgcccccctg acgagcatca caaaaataga 5640 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 5700 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 5760 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 5820 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 5880 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 5940 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 6000 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 6060 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 6120 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 6180 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 6240 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 6300 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 6360 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 6420 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 6480 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 6540 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 6600 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 6660 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 6720 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 6780 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 6840 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 6900 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 6960 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 7020 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 7080 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 7140 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 7200 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 7260 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 7320 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 7380 acctataaaa ataggcgtat cacgaggccc tttcgtc 7417 //