LOCUS       pLT-mCASP-1p30-1        4493 bp    DNA     circular     10-OCT-2011
DEFINITION  LMBP 3805.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3805
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 10-OCT-2011
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|603648636|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLT-mCASP-1p30-1|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Foreign object. Author: LMBP Plasmid collection. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                                   4.64101 4.70803) 
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FEATURES             Location/Qualifiers
     promoter        24..40
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    41..103
                     /vntifkey="21"
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
     CDS             167..1006
                     /vntifkey="4"
                     /label=mCASP-1\p30
                     /note="p30 fragment of the mouse caspase-1 cDNA"
     misc_marker     1013..1039
                     /vntifkey="22"
                     /label=Strep-tag
     terminator      1122..1169
                     /vntifkey="43"
                     /label=T7\terminator
     terminator      1243..1290
                     /vntifkey="43"
                     /label=T7\terminator
     terminator      1359..1440
                     /vntifkey="43"
                     /label=fd\terminator
     terminator      1469..1550
                     /vntifkey="43"
                     /label=fd\terminator
     rep_origin      1648..2103
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(2107..2480)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3021..3881)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4247..4377
                     /vntifkey="30"
                     /label=PL\promoter
     misc_marker     113..130
                     /vntifkey="22"
                     /label=His-tag
     misc_feature    131..145
                     /vntifkey="21"
                     /label=EK\recognition\site
     promoter        complement(1088..1104)
                     /vntifkey="30"
                     /label=T3\promoter
BASE COUNT     1202 a      1067 c      1045 g      1179 t 
ORIGIN
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca 
      121 tcaccatcac gacgatgacg ataaggcatc ggtacctgcc accatgggca catttccagg 
      181 actgactggg accctcaagt tttgcccttt agaaaaagcc cagaagttat ggaaagaaaa 
      241 tccttcagag atttatccaa taatgaatac aaccactcgt acacgtcttg ccctcattat 
      301 ctgcaacaca gagtttcaac atctttctcc gagggttgga gctcaagttg acctcagaga 
      361 aatgaagttg ctgctggagg atctggggta taccgtgaaa gtgaaagaaa atctcacagc 
      421 tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc ccagagcaca agacttctga 
      481 cagtactttc cttgtattca tgtctcatgg tatccaggag ggaatatgtg ggaccacata 
      541 ctctaatgaa gtttcagata ttttaaaggt tgacacaatc tttcagatga tgaacacttt 
      601 gaagtgccca agcttgaaag acaagcccaa ggtgatcatt attcaggcat gccgtggaga 
      661 gaaacaagga gtggtgttgt taaaagattc agtaagagac tctgaagagg atttcttaac 
      721 ggatgcaatt tttgaagatg atggcattaa gaaggcccat atagagaaag attttattgc 
      781 tttctgctct tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt 
      841 cattgagtca ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat 
      901 tttcagaaag gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc 
      961 tgatagggtg accctgacaa aacgtttcta cctcttcccg ggacatgcta gcgcttggcg 
     1021 ccacccccag tttggtggtt aataggatcc ggcaattgaa ttcgagctcg gccgacttgg 
     1081 ccttcccttt agtgagggtt aataaacttg gtgagcaata actagcataa ccccttgggg 
     1141 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatgc gctcatacga 
     1201 tatgaacgtt gagactgccg ctgagttatc agtgagcaat aactagcata accccttggg 
     1261 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatagctt 
     1321 atcgctagag gtcgaaattc acctcgaaag caagctgata aaccgataca attaaaggct 
     1381 ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc 
     1441 aagctaattc acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag 
     1501 cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc aagctctgcc 
     1561 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
     1621 cagcttgtct gtaagcggat gcagatcacg cgccctgtag cggcgcatta agcgcggcgg 
     1681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
     1741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
     1801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     1861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     1921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     1981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     2041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 
     2101 tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     2221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     2281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     2341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     2401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     2461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     2521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     2581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     2641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     2701 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     2761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     2821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     2881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     2941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     3001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     3061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     3121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     3181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     3241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     3301 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 
     3361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     3421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     3481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     3541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     3601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 
     3661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     3721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     3781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     3841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     3901 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca 
     3961 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa 
     4021 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa 
     4081 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg 
     4141 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag 
     4201 acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctc tcacctacca 
     4261 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg 
     4321 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc 
     4381 agcaggacgc actgaccacc atgaaggtga cgctcttaaa attaagccct gaagaagggc 
     4441 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gaa 
//
