LOCUS       pPLT10mTNFT3            4411 bp    DNA     circular     25-FEB-2011
DEFINITION  LMBP 3586.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3586
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 25-FEB-2011
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|582804322|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPLT10mTNFT3|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                                   "@N" 1 4.70294 13.9386 0 -10 5.3797 1.11561 
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COMMENT                                    4.84391 4.93117) 0.4 0.082322 0.915356 1.8 
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COMMENT                                   "@N" 1 16.1171 1.74233 0 -10 5.3797 1.11561 
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COMMENT                                   "@N" 1 -9.13935 12.1963 0 -10 5.50366 1.11561 
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COMMENT                                   6.2163) 
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COMMENT                                   "5' UTR of phage T7 gene 10, incl. RBS" "@N" 
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COMMENT                                   10.2031 -10.454 0 -10 2.2808 1.11561 #244#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -3.00061 -13.9386 0 -10 4.06577 1.11561 
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COMMENT                      #248=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -795 100) 
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COMMENT                                   4.72729 4.79555) 
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COMMENT                            "Last modified: 25-FEB-2011" 65535 0 -4.51201 0 -10 
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FEATURES             Location/Qualifiers
     promoter        24..40
                     /vntifkey="30"
                     /label=T7\promoter
     RBS             41..103
                     /vntifkey="32"
                     /label=5'\UTR\of\phage\T7\gene\10,\incl.\RBS
     CDS             107..580
                     /vntifkey="4"
                     /label=mature\mTNF
     misc_feature    581..831
                     /vntifkey="21"
                     /label=3'\UTR\of\the\mTNF
     promoter        complement(1006..1022)
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      1040..1087
                     /vntifkey="43"
                     /label=T7\terminator
     terminator      1161..1208
                     /vntifkey="43"
                     /label=T7\terminator
     terminator      1277..1358
                     /vntifkey="43"
                     /label=fd\terminator
     terminator      1387..1468
                     /vntifkey="43"
                     /label=fd\\terminator
     rep_origin      1566..2021
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     rep_origin      complement(2025..2398)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2939..3799)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4165..4295
                     /vntifkey="30"
                     /label=PL\promoter
BASE COUNT     1125 a      1122 c      1048 g      1116 t 
ORIGIN
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatcatgg taagatcaag 
      121 tagtcaaaat tcgagtgaca agcctgtagc ccacgtcgta gcaaaccacc aagtggagga 
      181 gcagctggag tggctgagcc agcgcgccaa cgccctcctg gccaacggca tggatctcaa 
      241 agacaaccaa ctagtggtgc cagccgatgg gttgtacctt gtctactccc aggttctctt 
      301 caagggacaa ggctgccccg actacgtgct cctcacccac accgtcagcc gatttgctat 
      361 ctcataccag gagaaagtca acctcctctc tgccgtcaag agcccctgcc ccaaggacac 
      421 ccctgagggg gctgagctca aaccctggta tgagcccata tacctgggag gagtcttcca 
      481 gctggagaag ggggaccaac tcagcgctga ggtcaatctg cccaagtact tagactttgc 
      541 ggagtccggg caggtctact ttggagtcat tgctctgtga agggaatggg tgttcatcca 
      601 ttctctaccc agcccccact ctgacccctt tactctgacc cctttattgt ctactcctca 
      661 gagcccccag tctgtgtcct tctaacttag aaaggggatt atggctcaga gtccaactct 
      721 gtgctcagag ctttcaacaa ctactcagaa acacaagatg ctgggacagt gacctggact 
      781 gtgggcctct catgcaccac catcaaggac tcaaatgggc tttccgaatt ctagaggtac 
      841 cggttgttaa cgttagccgg ctacgtatac tccggaatat taataggcct aggatgcata 
      901 tggcggccgc ctgcagctgg cgccatcgat acgcgtacgt cgcgaccgcg gacatgtaca 
      961 gagctcgagg tcgacgaatt cgagctcggc cgacttggcc ttccctttag tgagggttaa 
     1021 taaacttggt gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg 
     1081 ttttttgctg aaaggaggaa ctatatgcgc tcatacgata tgaacgttga gactgccgct 
     1141 gagttatcag tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg 
     1201 gttttttgct gaaaggagga actatatccg gatagcttat cgctagaggt cgaaattcac 
     1261 ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg 
     1321 gagattttca acgtgaaaaa attattattc gcaattccaa gctaattcac ctcgaaagca 
     1381 agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg gagattttca 
     1441 acgtgaaaaa attattattc gcaattccaa gctctgcctc gcgcgtttcg gtgatgacgg 
     1501 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 
     1561 agatcacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     1621 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     1681 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 
     1741 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     1801 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     1861 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     1921 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     1981 atttaacgcg aattttaaca aaatattaac gtttacaatt tgatctgcgc tcggtcgttc 
     2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     2341 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     2581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     3001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     3241 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     3541 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta tgtaagcaga cagttttatt 
     3841 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg 
     3901 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc 
     3961 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac 
     4021 aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag 
     4081 gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgccg gtgatgccgg 
     4141 ccacgatgcg tccggcgtag aggatctctc acctaccaaa caatgccccc ctgcaaaaaa 
     4201 taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg 
     4261 ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat 
     4321 gaaggtgacg ctcttaaaat taagccctga agaagggcag cattcaaagc agaaggcttt 
     4381 ggggtgtgtg atacgaaacg aagcattgga a 
//
