ID pLT10lacZT3 standard; circular DNA; ; 6997 BP. DT 17-JUN-2008 DE LMBP 3520. CC Accession number: LMBP 3520 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 17-JUN-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT promoter 24..40 FT /vntifkey="30" FT /label=T7\promoter FT RBS 41..103 FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT CDS 104..3160 FT /vntifkey="4" FT /label=lacZ FT /note="E. coli lac Z coding sequence; with modified 5' end" FT promoter complement(3592..3608) FT /vntifkey="30" FT /label=T3\promoter FT terminator 3626..3673 FT /vntifkey="43" FT /label=T7\terminator FT terminator 3747..3794 FT /vntifkey="43" FT /label=T7\terminator FT terminator 3863..3944 FT /vntifkey="43" FT /label=fd\terminator FT terminator 3973..4054 FT /vntifkey="43" FT /label=fd\terminator FT rep_origin 4152..4607 FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(4611..4984) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(5525..6385) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 6751..6881 FT /vntifkey="30" FT /label=PL\promoter SQ Sequence 6997 BP; 1678 A; 1784 C; 1787 G; 1742 t; 6 other; ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 60 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc ccgtcgtttt 120 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 180 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 240 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 300 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 360 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 420 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 480 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca 540 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt 600 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgttg 660 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga 720 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt 780 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg 840 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac 900 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact 960 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc 1020 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg 1080 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat 1140 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac 1200 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca 1260 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga 1320 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg 1380 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc 1440 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct 1500 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc 1560 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc 1620 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg 1680 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa 1740 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt 1800 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga 1860 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg 1920 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt 1980 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct 2040 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga 2100 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc 2160 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc 2220 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc 2280 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct 2340 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg 2400 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa 2460 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa 2520 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc 2580 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat 2640 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga 2700 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt 2760 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac 2820 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt 2880 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg 2940 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag 3000 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat 3060 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcgnnnnnnc agctgagcgc 3120 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg 3180 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg 3240 gatgttcggt ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt 3300 cccgatttgg ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc 3360 cgctatttct ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact 3420 cgggctgcgc aaatacctgc tgtggattat taccggcatg ttagtgatgt ttgcgccgtt 3480 ctttattttt atcttcgggc cactgttaca atacaacatt ttagtaggat cgattgttgg 3540 tggtatttat ctaggctttt gttttaacgc cggggccgac ttggccttcc ctttagtgag 3600 ggttaataaa cttggtgagc aataactagc ataacccctt ggggcctcta aacgggtctt 3660 gaggggtttt ttgctgaaag gaggaactat atgcgctcat acgatatgaa cgttgagact 3720 gccgctgagt tatcagtgag caataactag cataacccct tggggcctct aaacgggtct 3780 tgaggggttt tttgctgaaa ggaggaacta tatccggata gcttatcgct agaggtcgaa 3840 attcacctcg aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt 3900 tttttggaga ttttcaacgt gaaaaaatta ttattcgcaa ttccaagcta attcacctcg 3960 aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt tttttggaga 4020 ttttcaacgt gaaaaaatta ttattcgcaa ttccaagctc tgcctcgcgc gtttcggtga 4080 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 4140 ggatgcagat cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 4200 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 4260 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 4320 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 4380 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 4440 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 4500 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 4560 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttgat ctgcgctcgg 4620 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 4680 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 4740 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 4800 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 4860 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 4920 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 4980 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 5040 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 5100 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 5160 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 5220 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 5280 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 5340 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 5400 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 5460 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 5520 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 5580 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 5640 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 5700 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 5760 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 5820 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 5880 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 5940 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 6000 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 6060 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 6120 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 6180 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 6240 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 6300 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 6360 cgacacggaa atgttgaata ctcatactct tcctttttca atattatgta agcagacagt 6420 tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac 6480 acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca gatcacgcat 6540 cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac caactggtcc 6600 acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga tgatggggcg 6660 attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca gcgccggtga 6720 tgccggccac gatgcgtccg gcgtagagga tctctcacct accaaacaat gcccccctgc 6780 aaaaaataaa ttcatataaa aaacatacag ataaccatct gcggtgataa attatctctg 6840 gcggtgttga cataaatacc actggcggtg atactgagca catcagcagg acgcactgac 6900 caccatgaag gtgacgctct taaaattaag ccctgaagaa gggcagcatt caaagcagaa 6960 ggctttgggg tgtgtgatac gaaacgaagc attggaa 6997 //