LOCUS       pLT10TH                 3653 bp    DNA     circular      3-JUN-2008
DEFINITION  LMBP 3385.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3385
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 3-JUN-2008
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|484160039|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLT10TH|
COMMENT     VNTAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "HindII" "gtyrac" 4 0 3 215 0 2796 0 3505 0 0 
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "41..103") 
COMMENT        #6=(CFSignal (CObList) "T3 promoter" 30 0 1 248 264 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(248..265)") 
COMMENT        #7=(CFSignal (CObList) "T7 terminator" 43 0 0 282 329 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "282..329") 
COMMENT        #8=(CFSignal (CObList) "T7 terminator" 43 0 0 403 450 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "403..450") 
COMMENT        #9=(CFSignal (CObList) "fd terminator" 43 0 0 519 600 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "519..600") 
COMMENT        #10=(CFSignal (CObList) "fd terminator" 43 0 0 629 710 0 (CStringList) 
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COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 1267 1640 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "PL promoter" 30 0 0 3407 3537 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pLT10TH'" 1) 
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COMMENT                          "Currently local object. Original author: LMBP Plasmid Collection" 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3653 bp" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.lmbp@dmbr.UGent.be\">bccm.lmbp@dmbr.UGent.be</a>" 
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FEATURES             Location/Qualifiers
     promoter        24..40
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     RBS             41..103
                     /vntifkey="32"
                     /label=5'\UTR\of\T7\gene\10,\incl.\RBS
                     /note="5' UTR of phage T7 gene 10, incl. RBS"
     promoter        complement(248..264)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="phage T3 promoter"
     terminator      282..329
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="phage T7 gene 10 terminator"
     terminator      403..450
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="phage T7 gene 10 terminator"
     terminator      519..600
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="phage fd terminator"
     terminator      629..710
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="phage fd terminator"
     rep_origin      808..1263
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     rep_origin      complement(1267..1640)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="E. coli plasmid pMB1 origin"
     CDS             complement(2181..3041)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     promoter        3407..3537
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="phage lambda major leftward promoter"
     misc_marker     113..130
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     131..145
                     /vntifkey="22"
                     /label=EK\recognition\site
                     /note="enterokinase recognition site"
BASE COUNT      949 a       900 c       858 g       946 t 
ORIGIN
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca 
      121 tcaccatcac gacgatgacg ataaggcgcc cgggttcgaa atcgataagc ttatgcatgc 
      181 ggccgcatct agagggcccg gatccctcga ggtcgacgaa ttcgagctcg gccgacttgg 
      241 ccttcccttt agtgagggtt aataaacttg gtgagcaata actagcataa ccccttgggg 
      301 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatgc gctcatacga 
      361 tatgaacgtt gagactgccg ctgagttatc agtgagcaat aactagcata accccttggg 
      421 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatagctt 
      481 atcgctagag gtcgaaattc acctcgaaag caagctgata aaccgataca attaaaggct 
      541 ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc 
      601 aagctaattc acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag 
      661 cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc aagctctgcc 
      721 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
      781 cagcttgtct gtaagcggat gcagatcacg cgccctgtag cggcgcatta agcgcggcgg 
      841 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 
      901 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 
      961 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 
     1021 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 
     1081 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 
     1141 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 
     1201 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 
     1261 tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     1321 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     1381 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     1441 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     1501 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     1561 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     1621 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     1681 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     1741 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     1801 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     1861 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     1921 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     1981 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     2041 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     2101 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     2161 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     2221 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     2281 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     2341 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     2401 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     2461 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 
     2521 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     2581 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     2641 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     2701 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     2761 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 
     2821 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     2881 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     2941 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     3001 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     3061 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca 
     3121 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa 
     3181 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa 
     3241 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg 
     3301 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag 
     3361 acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctc tcacctacca 
     3421 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg 
     3481 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc 
     3541 agcaggacgc actgaccacc atgaaggtga cgctcttaaa attaagccct gaagaagggc 
     3601 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gaa 
//
