ID pLT10T3 standard; circular DNA; ; 3654 BP. DT 29-OCT-2007 DE LMBP 3384. CC Accession number: LMBP 3384 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 18-JUN-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT promoter 24..40 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT misc_feature 41..103 FT /vntifkey="21" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT /note="5' UTR of phage T7 gene 10, incl. ribosome binding site" FT promoter complement(249..265) FT /vntifkey="30" FT /label=T3\promoter FT terminator 283..330 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 404..451 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 520..601 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT terminator 630..711 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT rep_origin 809..1264 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT rep_origin complement(1268..1641) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(2182..3042) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 3408..3538 FT /vntifkey="30" FT /label=PL\promoter FT /note="phage lambda major leftward promoter" SQ Sequence 3654 BP; 943 A; 900 C; 862 G; 949 t; ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 60 ctctagaaat aattttgttt aactttaaga aggagatata catatgggcc cgacgtcgca 120 tgctcctcta gactcgagga attcggtacc ccgggttcga aatcgataag cttatgcatg 180 cggccgcatc tagagggccc ggatccctcg aggtcgacga attcgagctc ggccgacttg 240 gccttccctt tagtgagggt taataaactt ggtgagcaat aactagcata accccttggg 300 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg cgctcatacg 360 atatgaacgt tgagactgcc gctgagttat cagtgagcaa taactagcat aaccccttgg 420 ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatagct 480 tatcgctaga ggtcgaaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc 540 tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc 600 caagctaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga 660 gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctctgc 720 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 780 acagcttgtc tgtaagcgga tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg 840 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct 900 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 960 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 1020 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 1080 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 1140 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta 1200 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 1260 atttgatctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 1320 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 1380 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 1440 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 1500 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 1560 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 1620 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 1680 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 1740 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 1800 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 1860 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 1920 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 1980 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 2040 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 2100 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 2160 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 2220 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 2280 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 2340 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 2400 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 2460 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc 2520 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 2580 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 2640 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 2700 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 2760 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 2820 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 2880 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 2940 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 3000 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 3060 ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac 3120 atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga 3180 aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca 3240 aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct 3300 ggctggatga tggggcgatt caggcctggt atgagtcagc aacaccttct tcacgaggca 3360 gacctcagcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct ctcacctacc 3420 aaacaatgcc cccctgcaaa aaataaattc atataaaaaa catacagata accatctgcg 3480 gtgataaatt atctctggcg gtgttgacat aaataccact ggcggtgata ctgagcacat 3540 cagcaggacg cactgaccac catgaaggtg acgctcttaa aattaagccc tgaagaaggg 3600 cagcattcaa agcagaaggc tttggggtgt gtgatacgaa acgaagcatt ggaa 3654 //