ID pLOF/Km standard; circular DNA; ; 10146 BP. DT 15-OCT-2008 DE LMBP 3337. CC Accession number: LMBP 3337 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 15-OCT-2008 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT rep_origin complement(1062..1172) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT rep_origin complement(63..441) FT /vntifkey="33" FT /label=R6K\ori FT CDS complement(2643..3503) FT /vntifkey="4" FT /label=ampicillin\resistance FT CDS complement(3649..4731) FT /vntifkey="4" FT /label=lacI FT promoter complement(4732..4809) FT /vntifkey="30" FT /label=lacIq\promoter FT /note="mutated E. coli repressor lacI promoter, incl. RBS" FT misc_feature 9172..9241 FT /vntifkey="21" FT /label=IS10\IR FT /note="IS10 inverted repeat" FT misc_feature 7347..7416 FT /vntifkey="21" FT /label=IS10\IR FT /note="IS10 inverted repeat" FT CDS 5138..6346 FT /vntifkey="4" FT /label=IS10R FT /note="coding sequence of the IS10R (insertion sequence 10 right) transposase gene" FT CDS complement(7858..8673) FT /vntifkey="4" FT /label=Tn903\neomycin\resistance FT RBS 5061..5096 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT promoter 5018..5060 FT /vntifkey="30" FT /label=tac\promoter SQ Sequence 10146 BP; 2677 A; 2532 C; 2391 G; 2540 t; 6 other; aattcgatcg ctagtttgtt ttgactccat ccattagggc ttctaaaacg ccttctaagg 60 ccatgtcagc cgttaagtgt tcctgtgtca ctgaaaattg ctttgagagg ctctaagggc 120 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa 180 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg 240 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct 300 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg 360 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac 420 tatcaacagg ttgaactgct ggatcctttt tgtccggtgt tgggttgaag gtgaagccgg 480 tcggggccgc agcgggggcc ggcttttcag ccttgccccc ctgcttcggc cgccgtggct 540 ccggcgtctt gggtgccggc gcgggttccg cagccttggc ctgcggtgcg ggcacatcgg 600 cgggcttggc cttgatgtgc cgcctggcgt gcgagcggaa cgtctcgtag gagaacttga 660 ccttccccgt ttcccgcatg tgctcccaaa tggtgacgag cgcatagccg gacgctaacg 720 ccgcctcgac atccgccctc accgccagga acgcaaccgc agcctcatca cgccggcgct 780 tcttggccgc gcgggattca acccactcgg ccagctcgtc ggtgtagctc tttggcatcg 840 tctctcgcct gtcccctcag ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga 900 tattccacaa aacagcaggg aagcagcgct tttccgctgc ataaccctgc ttcggggtca 960 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa 1020 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag 1080 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc 1140 tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca 1200 agcggatggc tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact 1260 gccttccaga cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc atgagcctgt 1320 cggcctacct gctggccgtc ggccagggct acaaaatcac gggcgtcgtg gactatgagc 1380 acgtccgcga gctggcccgc atcaatggcg acctgggccg cctgggcggc ctgctgaaac 1440 tctggctcac cgacgacccg cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc 1500 tggcgaagat cgaagagaag caggacgagc ttggcaaggt catgatgggc gtggtccgcc 1560 cgagggcaga gccatgactt ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc 1620 aagcacgtcc ccatgcgctc catcaagaag agcgacttcg cggagctggt gaagtacatc 1680 accgacgagc aaggcaagac cgagcgcctg ggtcacgtgc gcgtcacgaa ctgcgaggca 1740 aacaccctgc ccgctgtcat ggccgaggtg atggcgaccc agcacggcaa cacccgttcc 1800 gaggccgaca agacctatca cctgctggtt agcttccgcg cgggagagaa gcccgacgcg 1860 gagacgttgc gcgcgattga ggaccgcatc tgcgctgggc ttggcttcgc cgagcatcag 1920 cgcgtcagtg ccgtgcatca cgacaccgac aacctgcaca tccatatcgc catcaacaag 1980 attcacccga cccgaaacac catccatgag ccgtatcggg cctaccgcgc cctcgctgac 2040 ctctgcgcga cgctcgaacg ggactacggg cttgagcgtg acaatcacga aacgcggcag 2100 cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc 2160 ggctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt 2220 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 2280 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 2340 gccatctcct tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 2400 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 2460 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 2520 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 2580 caaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2640 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2700 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2760 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 2820 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 2880 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 2940 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 3000 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 3060 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 3120 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 3180 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 3240 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 3300 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 3360 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 3420 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 3480 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3540 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3600 gttccgcgca catttccccg aaaagtgcca cctgcagatc tgcagnnntc actgcccgct 3660 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 3720 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 3780 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 3840 ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc 3900 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt 3960 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 4020 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 4080 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 4140 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 4200 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 4260 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 4320 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 4380 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 4440 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 4500 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 4560 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 4620 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 4680 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 4740 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc 4800 gatggtgtcn nngagctctc ccgggaattc tcatgtttga cagcttatca tcgactgcac 4860 ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt 4920 cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt 4980 tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca 5040 tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacaggatc 5100 aaatggtttc gcgaaaaatc aataatcaga caacaagatg tgcgaactcg atattttaca 5160 cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt 5220 ggcttgccac gcattacttg actgtaaaac tctcactctt accgaacttg gccgtaacct 5280 gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg 5340 tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg 5400 caatacgatg cccattgtac ttgttgactg gtctgatatt cgtgagcaaa aacgacttat 5460 ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt 5520 cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag 5580 cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg 5640 gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca 5700 atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc 5760 tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca 5820 aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca 5880 ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat gggttctagc 5940 aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg 6000 aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg 6060 ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct 6120 tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt 6180 ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt 6240 tttgcggcat tctggctaca caataacaag ggaagactta ctcgtggctg caaccctact 6300 agctcaaaat ttattcacac atggttacgc tttggggaaa ttatgagggg atctctcagt 6360 gcattgcctc caattcccat aatttattac gccgataata acttggtgta accttaaaaa 6420 tgtacttaaa tcgacgtgta aaagattgtt gggaatcaaa ttgatatttt aatgcgatct 6480 caaggatagt ttttttcgtc aacctcaact caacagccgc tttcgtcaaa cgacgagcac 6540 gaatatagct agccagtgtg acccctgtta ctttttgaac agccgctgaa aataccactt 6600 ggtataaccc gctttattcg ccacatcatc aagcagtaaa gactgatcta aattatgttc 6660 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa 6720 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc 6780 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta 6840 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc 6900 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt 6960 tcaatagatg agatcgatct cgggaaaagc gttggtgacc aaaggtgcct tttatcatca 7020 ctttaaaaat aaaaaacaat tactcagtgc ctgttataag cagcaattaa ttatgattga 7080 tgcctacatc acaacaaaaa ctgatttaac aaatggttgg tctgccttag aaagtatatt 7140 tgaacattat cttgattata ttattgataa taataaaaac cttatcccta tccaagaagt 7200 gatgcctatc attggttgga atgaacttga aaaaattagc cttgaataca ttactggtaa 7260 ggtaaacgcc attgtcagca aattgatcca agagaaccaa cttaaagctt tcctgacgga 7320 atgttaattc tcgttgaccc tgagcactga tgaatcccct aatgattttg gtaaaaatca 7380 ttaagttaag gtggatacac atcttgtcat atgatcccgg atccggctgt aatccgggca 7440 gcgcaacgga acattcatca gtgtaaaaat ggaatcaata aagccctgcg cagcgcgcag 7500 ggtcagcctg aatacgcgtg cggccgccgc gtttaatgac cagcacagtc gtgatggcaa 7560 ggtcagaata gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct 7620 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt 7680 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg 7740 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc 7800 gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta 7860 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 7920 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 7980 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 8040 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 8100 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc 8160 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 8220 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 8280 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc 8340 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc 8400 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 8460 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 8520 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt 8580 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct 8640 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 8700 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag 8760 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca 8820 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac 8880 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga 8940 tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca 9000 gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcggcg gccgcggcct 9060 aggcggccac gcgtattcag gctgaccctg cgcgctgcgc agggctttat tgattccatt 9120 tttacactga tgaatgttcc gttgcgctgc ccggattaca gccggatccg ggatcatatg 9180 acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca 9240 gtgctcaggg tcaacgagaa ttaacattcc gtcaggaaag cttatgatga tgatgtgctt 9300 aaaaacttac tcaatggctg gtttatgcat atcgcaatac atgcgaaaaa cctaaaagag 9360 cttgccgata aaaaaggcca atttattgct atttaccgcg gctttttatt gagcttgaaa 9420 gataaataaa atagataggt tttatttgaa gctaaatctt ctttatcgta aaaaatgccc 9480 tcttgggtta tcaagagggt cattatattt cgcggaataa catcatttgg tgacgaaata 9540 actaagcact tgtctcctgt ttactcccct gagcttgagg ggttaacatg aaggtcatcg 9600 atagcaggat aataatacag taaaacgcta aaccaataat ccaaatccag ccatcccaaa 9660 ttggtagtga atgattataa ataacagcaa acagtaatgg gccaataaca ccggttgcat 9720 tggtaaggct caccaataat ccctgtaaag caccttgctg atgactcttt gtttggatag 9780 acatcactcc ctgtaatgca ggtaaagcga tcccaccacc agccaataaa attaaaacag 9840 ggaaaactaa ccaaccttca gatataaacg ctaaaaaggc aaatgcacta ctatctgcaa 9900 taaatccgag cagtactgcc gttttttcgc cccatttagt ggctattctt cctgccacaa 9960 aggcttggaa tactgagtgt aaaagaccaa gacccgctaa tgaaaagcca accatcatgc 10020 tattccatcc aaaacgattt tcggtaaata gcacccacac cgttgcggga atttggccta 10080 tcaattgcgc tgaaaaataa ataatcaaca aaatgggcat cgttttaaat aaagtgatgt 10140 ataccg 10146 //