LOCUS       pBR325                  5994 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 324.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 324
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|461008300|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBR325|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CObList) -1 "") 
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COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "HindII" "gtyrac" 4 0 2 1869 0 5123 0 0 "")) 
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COMMENT            (CStringList) 1 1 1 1 "1301..2491") 
COMMENT        #3=(CFSignal (CObList) "pMB1 ori" 33 0 1 3565 3967 0 (CStringList) 
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COMMENT        #4=(CFSignal (CObList) "ampicillin" 4 0 1 4508 5368 0 (CStringList) 
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COMMENT                         "DNA Plasmid 'pBR325'" 1) 
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COMMENT                         "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modified: 10/23/07 05:51" 1) 
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COMMENT                         "length: 5994 bp" 1) 
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COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
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FEATURES             Location/Qualifiers
     CDS             1301..2491
                     /vntifkey="4"
                     /label=tetracycline
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(3565..3967)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4508..5368)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     CDS             5779..444
                     /vntifkey="4"
                     /label=chloramphenicol
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1394 a      1600 c      1565 g      1435 t 
ORIGIN
        1 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 
       61 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 
      121 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 
      181 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 
      241 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 
      301 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 
      361 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 
      421 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 
      481 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt 
      541 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt 
      601 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca 
      661 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc 
      721 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc 
      781 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg 
      841 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg 
      901 acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc 
      961 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg 
     1021 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac 
     1081 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg 
     1141 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg 
     1201 aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt aatgcggtag 
     1261 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct 
     1321 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt 
     1381 actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt 
     1441 gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc 
     1501 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta 
     1561 cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg 
     1621 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 
     1681 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 
     1741 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 
     1801 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 
     1861 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 
     1921 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 
     1981 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 
     2041 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 
     2101 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga 
     2161 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat 
     2221 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca 
     2281 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac 
     2341 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa 
     2401 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg 
     2461 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 
     2521 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 
     2581 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc 
     2641 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc 
     2701 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga 
     2761 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc 
     2821 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc 
     2881 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg 
     2941 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt 
     3001 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg 
     3061 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg 
     3121 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc 
     3181 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc 
     3241 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt 
     3301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 
     3361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 
     3421 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 
     3481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 
     3541 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     3601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     3661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     4201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4801 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     4861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     4921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     4981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     5041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     5101 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 
     5161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     5221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     5281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     5401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     5521 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt cgaataaata 
     5581 cctgtgacgg aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg 
     5641 aagccctggg ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac 
     5701 tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca 
     5761 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc 
     5821 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac 
     5881 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag 
     5941 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaa 
//
