LOCUS       pBLCAT5HIL6305          4545 bp    DNA     circular     21-AUG-2013
DEFINITION  LMBP 3232.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3232
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|670933491|
COMMENT     VNTDBDATE|670933491|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBLCAT5HIL6305|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 32 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 341 0 0 "")) 
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COMMENT        #4=(CFSignal (CObList) "intron of SV40 small t-antigen" 15 0 0 1207 1273 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "1066..1132") 
COMMENT        #5=(CFSignal (CObList) "3' UTR of SV40 small t-antigen" 21 0 0 1274 1825 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "1133..1684") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1826 1922 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1685..1781") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1826 1922 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1685..1781)") 
COMMENT        #8=(CFSignal (CObList) "lac promoter" 30 0 1 2061 2144 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1920..2003)") 
COMMENT        #9=(CFSignal (CObList) "pMB1 ori" 33 0 1 2236 2638 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3179 4039 0 
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COMMENT        #11=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4171 4245 0 
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COMMENT        #15=(CFSignal (CObList) "hIL6 promoter" 29 0 0 36 331 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pBLCAT5HIL6305'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 08/21/13 10:24" 1) 
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COMMENT                          "Last Modified: 08/21/13 10:24" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4545 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 10-FEB-2009" 1))) 
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COMMENT                                        "chloramphenicol acetyltransferase coding sequence" 
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FEATURES             Location/Qualifiers
     CDS             383..1042
                     /vntifkey="4"
                     /label=cat
                     /note="chloramphenicol acetyltransferase coding sequence"
     CDS             1132..1206
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
     intron          1207..1273
                     /vntifkey="15"
                     /label=intron\of\SV40\small\t-antigen
     misc_feature    1274..1825
                     /vntifkey="21"
                     /label=3'\UTR\of\SV40\small\t-antigen
     terminator      1826..1922
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1826..1922)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(2061..2144)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2236..2638)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3179..4039)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     terminator      4171..4245
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4171..4245)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      4386..4482
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4386..4482)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        36..331
                     /vntifkey="29"
                     /label=hIL6\promoter
BASE COUNT     1254 a      1030 c       983 g      1278 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccatgc caaagtgctg agtcactaat 
       61 aaaagaaaaa aagaaagtaa aggaagagtg gttctgcttc ttagcgctag cctcaatgac 
      121 gacctaagct gcacttttcc ccctagttgt gtcttgcgat gctaaaggac gtcacattgc 
      181 acaatcttaa taaggtttcc aatcagcccc acccgctctg gccccaccct caccctccaa 
      241 caaagattta tcaaatgtgg gattttccca tgagtctcaa tattagagtc tcaaccccca 
      301 ataaatatag gactggagat gtctgaggct cattctgccc tcgaggagct tggcgagatt 
      361 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat 
      421 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta 
      481 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca 
      541 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt 
      601 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac 
      661 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt 
      721 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta 
      781 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt 
      841 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat 
      901 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca 
      961 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga 
     1021 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg 
     1081 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgcc ggatctttgt 
     1141 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 
     1201 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 
     1261 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt 
     1321 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc 
     1381 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 
     1441 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg 
     1501 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa 
     1561 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct 
     1621 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt 
     1681 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 
     1741 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 
     1801 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 
     1861 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 
     1921 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 
     1981 tcgatccccg ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa 
     2041 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 
     2101 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 
     2161 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 
     2221 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     2281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     2341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     2401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     2461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     2521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     2581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     2641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     2701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     2761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     2821 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     2881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     2941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     3001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     3061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     3121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     3181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     3241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     3301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     3361 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     3421 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     3481 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     3541 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     3601 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     3661 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     3721 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     3781 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     3841 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     3901 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     3961 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     4021 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     4081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     4141 cgcgcacatt tccccgaaaa gtgccacctg aacttgttta ttgcagctta taatggttac 
     4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcataat cagccatacc 
     4321 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctcg 
//
