LOCUS       pBRphoAE6Hf1            4569 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2957.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBRphoAE6Hf1|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBRphoAE6Hf1" 0 4569 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT        #5=(CFSignal (CObList) "sPHOA" 21 0 0 129 191 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "VH" 4 0 0 192 551 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "192..551") 
COMMENT        #7=(CFSignal (CObList) "CH1" 4 0 0 552 857 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "552..857") 
COMMENT        #8=(CFSignal (CObList) "ORI" 33 0 1 2560 2960 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2560..2960)") 
COMMENT        #9=(CFSignal (CObList) "AMP" 4 0 1 3501 4361 0 (CStringList) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4569 bp" 1) 
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COMMENT                                   #28=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "5' UTR containing the RBS of the E. coli phoA gene" 
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COMMENT                                        "E. coli hybrid tryptophan/lac operon promoter" 
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FEATURES             Location/Qualifiers
     promoter        31..65
                     /vntifkey="30"
                     /label=tac
                     /note="E. coli hybrid tryptophan/lac operon promoter"
     misc_feature    66..128
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR containing the RBS of the E. coli phoA gene"
     misc_feature    129..191
                     /vntifkey="21"
                     /label=sPHOA
                     /note="E. coli alkaline phosphatase A signal sequence"
     CDS             192..551
                     /vntifkey="4"
                     /label=VH
                     /note="variable domain of E6H"
     CDS             552..857
                     /vntifkey="4"
                     /label=CH1
                     /note="constant domain 1 of E6H"
     rep_origin      complement(2560..2960)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3501..4361)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1075 a      1254 c      1168 g      1072 t 
ORIGIN
        1 ttcgagctcg agcttactcc ccatccccct gttgacaatt aatcatcggc tcgtataatg 
       61 tgtggaattg tgagcggata acaatttcac acaggaaaca ggatccgcgg atccgtggag 
      121 aaaataaagt gaaacaaagc actattgcac tggcactctt accgttactg tttacccctg 
      181 tgacaaaagc gcaggttcag ctgcagcagt ctggagctga gctggcgagg cctggggcct 
      241 cagtgaagct gtcctgcaag gcctctggat acaccctcac aagttatggt ataagttggg 
      301 tgaagcagag aactggacag ggccttgagt ggattggaga aatttatcct ggaagtggaa 
      361 attcgtactt caatgagaag tttaagggca aggccacact gactgtagac aaatcctcca 
      421 gcacagccta cttgcacctc agcagcctga catctgagga ctctgccgtc tatttctgtg 
      481 ccggacctag acaggtcggg ctactccctt ttggttactg gggccaaggg actctggtca 
      541 ctgcctctgc agccaaaaca acacccccat cagtctatcc actggcccct gggtgtggag 
      601 atacaactgg ttcctccgtg actctgggat gcctggtcaa gggctacttc cctgagtcag 
      661 tgactgtgac ttggaactcc ggatccctgt ccagcagtgt gcacaccttc ccagctctcc 
      721 tgcagtctgg actctacact atgagcagct cagtgactgt cccctccagc acctggccaa 
      781 gccagaccgt tacctgcagt gttgctcacc cagccagcag caccacggtg gacaaaaaac 
      841 ttgagcccag cgtctagagt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct 
      901 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc 
      961 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct 
     1021 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc 
     1081 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg 
     1141 gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg 
     1201 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca 
     1261 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc 
     1321 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg 
     1381 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc 
     1441 ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa gccggcggca 
     1501 cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc ggagaactgt 
     1561 gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct ccagcagccg 
     1621 cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct 
     1681 gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc 
     1741 gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac 
     1801 atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg 
     1861 caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac 
     1921 atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat 
     1981 ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt 
     2041 aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa 
     2101 tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg 
     2161 ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga 
     2221 acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct 
     2281 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 
     2341 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 
     2401 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg 
     2461 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 
     2521 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 
     2581 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 
     2641 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 
     2701 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 
     2761 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 
     2821 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 
     2881 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 
     2941 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 
     3001 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 
     3061 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 
     3121 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 
     3181 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 
     3241 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 
     3301 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 
     3361 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 
     3421 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 
     3481 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 
     3541 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 
     3601 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 
     3661 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 
     3721 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 
     3781 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 
     3841 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 
     3901 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 
     3961 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 
     4021 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 
     4081 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 
     4141 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 
     4201 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 
     4261 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 
     4321 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 
     4381 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 
     4441 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 
     4501 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 
     4561 cttcaagaa 
//
