LOCUS       pPLaA17                 5241 bp    DNA     circular     25-FEB-2013
DEFINITION  LMBP 295.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 295
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 25-FEB-2013
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|654704644|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPLaA17|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPLaA17" 0 5241 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "StyI" "AvaI" "EcoRI" "HindIII" "PvuI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5241 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
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COMMENT        #1=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 3 645 0 919 0 4660 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 3715 0 5240 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1165 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 1047 0 3092 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "PL promoter" 30 0 1 117 247 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(117..247)") 
COMMENT        #6=(CFSignal (CObList) "Tn903 neomycin resistance" 4 0 0 615 1430 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "615..1430") 
COMMENT        #7=(CFSignal (CObList) "ColE1 ori" 33 0 1 1716 2107 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1716..2107)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2648 3508 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2648..3508)") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of A" 21 0 0 3717 3740 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3717..3740") 
COMMENT        #10=(CFSignal (CObList) "A" 4 0 0 3741 4922 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3741..4922") 
COMMENT        #11=(CFSignal (CObList) "5' UTR of COAT" 21 0 0 4923 4945 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4923..4945") 
COMMENT        #12=(CFSignal (CObList) "COATf" 4 0 0 4946 5241 0 (CStringList) 
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COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #13=(CFSignal (CObList) "LMBP 295" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #14=(CFSignal (CObList) "Last modified: 25-FEB-2013" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pPLaA17'" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 295." 
COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/25/13 02:24" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5241 bp" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 5-NOV-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "Tn903 neomycin resistance") 
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COMMENT                                 "ampicillin resistance") 
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COMMENT                                   #47=(CLinePar 
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COMMENT                                        "MS2 assembly protein coding sequence" 1) 
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COMMENT                                        "Start: 4946  End: 5241" 1) 
COMMENT                                   #52=(CLinePar 
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COMMENT                                        "first 97 codons of the MS2 coat protein gene" 
COMMENT                                        1) 
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COMMENT                                        "phage lambda major leftward promoter" 1) 
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COMMENT                                 "PL promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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FEATURES             Location/Qualifiers
     promoter        complement(117..247)
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="phage lambda major leftward promoter"
     CDS             615..1430
                     /vntifkey="4"
                     /label=Tn903\neomycin\resistance
     rep_origin      complement(1716..2107)
                     /vntifkey="33"
                     /label=ColE1\ori
                     /note="origin plasmid"
     CDS             complement(2648..3508)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    3717..3740
                     /vntifkey="21"
                     /label=5'\UTR\of\A
                     /note="5' UTR of the MS2 assembly protein gene, incl. RBS"
     CDS             3741..4922
                     /vntifkey="4"
                     /label=A
                     /note="MS2 assembly protein coding sequence"
     misc_feature    4923..4945
                     /vntifkey="21"
                     /label=5'\UTR\of\COAT
                     /note="5' UTR of the MS2 coat protein gene, incl. RBS"
     CDS             4946..5241
                     /vntifkey="4"
                     /label=COATf
                     /note="first 97 codons of the MS2 coat protein gene"
BASE COUNT     1317 a      1273 c      1271 g      1380 t 
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc 
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc 
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga 
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg 
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc 
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa 
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga 
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac 
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga 
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc 
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     2941 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     3001 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     3061 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     3121 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     3181 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     3241 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 
     3301 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     3361 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     3421 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     3481 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     3541 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     3601 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     3661 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattcc 
     3721 attcctagga ggtttgacct gtgcgagctt ttagtaccct tgatagggag aacgagacct 
     3781 tcgtcccctc cgttcgcgtt tacgcggacg gtgagactga agataactca ttctctttaa 
     3841 aatatcgttc gaactggact cccggtcgtt ttaactcgac tggggccaaa acgaaacagt 
     3901 ggcactaccc ctctccgtat tcacgggggg cgttaagtgt cacatcgata gatcaaggtg 
     3961 cctacaagcg aagtgggtca tcgtggggtc gcccgtacga ggagaaagcc ggtttcggct 
     4021 tctccctcga cgcacgctcc tgctacagcc tcttccctgt aagccaaaac ttgacttaca 
     4081 tcgaagtgcc gcagaacgtt gcgaaccggg cgtcgaccga agtcctgcaa aaggtcaccc 
     4141 agggtaattt taaccttggt gttgctttag cagaggccag gtcgacagcc tcacaactcg 
     4201 cgacgcaaac cattgcgctc gtgaaggcgt acactgccgc tcgtcgcggt aattggcgcc 
     4261 aggcgctccg ctaccttgcc ctaaacgaag atcgaaagtt tcgatcaaaa cacgtggccg 
     4321 gcaggtggtt ggagttgcag ttcggttggt taccactaat gagtgatatc cagggtgcat 
     4381 atgagatgct tacgaaggtt caccttcaag agtttcttcc tatgagagcc gtacgtcagg 
     4441 tcggtactaa catcaagtta gatggccgtc tgtcgtatcc agctgcaaac ttccagacaa 
     4501 cgtgcaacat atcgcgacgt atcgtgatat ggttttacat aaacgatgca cgtttggcat 
     4561 ggttgtcgtc tctaggtatc ttgaacccac taggtatagt gtgggaaaag gtgcctttct 
     4621 cattcgttgt cgactggctc ctacctgtag gtaacatgct cgagggcctt acggcccccg 
     4681 tgggatgctc ctacatgtca ggaacagtta ctgacgtaat aacgggtgag tccatcataa 
     4741 gcgttgacgc tccctacggg tggactgtgg agagacaggg cactgctaag gcccaaatct 
     4801 cagccatgca tcgaggggta caatccgtat ggccaacaac tggcgcgtac gtaaagtctc 
     4861 ctttctcgat ggtccatacc ttagatgcgt tagcattaat caggcaacgg ctctctagat 
     4921 agagccctca accggagttt gaagcatggc ttctaacttt actcagttcg ttctcgtcga 
     4981 caatggcgga actggcgacg tgactgtcgc cccaagcaac ttcgctaacg gggtcgctga 
     5041 atggatcagc tctaactcgc gttcacaggc ttacaaagta acctgtagcg ttcgtcagag 
     5101 ctctgcgcag aatcgcaaat acaccatcaa agtcgaggtg cctaaagtgg caacccagac 
     5161 tgttggtggt gtagagcttc ctgtagccgc atggcgttcg tacttaaata tggaactaac 
     5221 cattccaatt ttcgctacga a 
//
