LOCUS       pBluhHemeOx             4480 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2866.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBluhHemeOx|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBluhHemeOx" 0 4480 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
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COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "f1ORI" 33 0 0 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #4=(CFSignal (CObList) "T7" 30 0 0 626 642 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "626..642") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 1 710 1312 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(710..1312)") 
COMMENT        #6=(CFSignal (CObList) "hHOx" 4 0 1 1313 2179 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1313..2179)") 
COMMENT        #7=(CFSignal (CObList) "5UTR" 21 0 1 2180 2216 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2180..2216)") 
COMMENT        #8=(CFSignal (CObList) "T3" 30 0 1 2294 2310 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2294..2310)") 
COMMENT        #9=(CFSignal (CObList) "lac" 30 0 1 2374 2457 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2374..2457)") 
COMMENT        #10=(CFSignal (CObList) "ORI" 33 0 1 2549 2951 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2549..2951)") 
COMMENT        #11=(CFSignal (CObList) "AMP" 4 0 1 3492 4352 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3492..4352)")) (CObList) 
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COMMENT                          "DNA 'pBluhHemeOx'" 1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4480 bp" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 1313  End: 2179 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human heme oxygenase coding sequence" 1) 
COMMENT                                   #31=(CLinePar 
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COMMENT                                   #32=(CLinePar 
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COMMENT                                        "   complement(1313..2179)" 1))) "hHOx") 
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COMMENT                                        "Start: 3492  End: 4352 (Complementary)" 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "   complement(3492..4352)" 1))) "AMP"))) 
COMMENT                          "CDS (2 total)") 
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COMMENT                                        "3' UTR of the human heme oxygenase gene" 
COMMENT                                        1) 
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COMMENT                                        "   complement(710..1312)" 1))) "3UTR") 
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COMMENT                                        "Start: 2180  End: 2216 (Complementary)" 
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COMMENT                                   #46=(CLinePar 
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COMMENT                                        "5' UTR of the human HOx gene" 1) 
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COMMENT                                        "phage T7 gene 10 promoter" 1) 
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COMMENT                          "Replication Origin (2 total)"))) "Feature Map")))) 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     promoter        626..642
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     misc_feature    complement(710..1312)
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human heme oxygenase gene"
     CDS             complement(1313..2179)
                     /vntifkey="4"
                     /label=hHOx
                     /note="human heme oxygenase coding sequence"
     misc_feature    complement(2180..2216)
                     /vntifkey="21"
                     /label=5UTR
                     /note="5' UTR of the human HOx gene"
     promoter        complement(2294..2310)
                     /vntifkey="30"
                     /label=T3
                     /note="phage T3 promoter"
     promoter        complement(2374..2457)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2549..2951)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3492..4352)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1033 a      1181 c      1177 g      1083 t         6 others
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 
      661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcnnnc agacaatgtt 
      721 gtttattatt tcacacaaaa gttagaccaa ggccacagtg ccgttaaaca cctccctccc 
      781 cacccacgca tggctcaaaa accaccccaa ccctgctata aaaacaacag aacacaacaa 
      841 aaatactgca tttgaggctg agccaggaac agagtggctc caacaaaaat aagacaaaaa 
      901 cacaagacac aacatctttt caggtttgtg taaaaattgc catggcccac ggggaaagtg 
      961 gtcatggccg tgtcaacaag gatacttgaa actcagggct tttggaggtt tgagacagct 
     1021 gccacattag ggtgtcttcc aggctctgct gcaggaactg aggatgctga agggcagggc 
     1081 tgaccccttc gccccctctg aagtttaggc cattgcctgg atgtgctttt cgttggggaa 
     1141 gatgccatag gctccttcct cctttccaga gagagggaca cagtgagagg gctggaggcc 
     1201 ctgaaagcct tcagtgccca cggtaaggaa gccagccaag agaattccct ctctagaaag 
     1261 aaggccttcc accggacaaa gttcatggcc ctgggagcca gcatgcctgc attcacatgg 
     1321 cataaagccc tacagcaact gtcgccacca gaaagctgag tgtaaggacc catcggagaa 
     1381 gcggagcctg ggagcgggtg ttgagtgggg gcttccctct gggagtctcc acgggggcag 
     1441 aatcttgcac tttgttgctg gcccgctggc gaagccctgg tgcccgtgag gggctctggt 
     1501 ccttggtgtc atgggtcagc agctcctgca actcctcaaa gagctggatg ttgagcagga 
     1561 acgcagtctt ggcctcttct atcaccctct gcctgactgc gggagtcatc tccagggagt 
     1621 tcatgcggga gcggtagagc tgcttgaact tggtggcact ggcaatgttg gggaaggtga 
     1681 agaaggccag gccctcgcca gagctgggca ggtccagggc tttctgggca atctttttga 
     1741 gcacctggcc cccagacagg tcacccaggt agcgggtgta ggcgtgggcc accagcagct 
     1801 cgggctctgt gcgccccacc tcgtggagcc gcttcacata gcgctgcatg gctggtgtgt 
     1861 aggggatgac ctcctgccag cggggcccgt accagaaggc caggtcctgc tccagggcag 
     1921 ccttgcggtg cagctcttct gggaagtaga caggggcgaa gactgggctc tccttgttgc 
     1981 gctcaatctc ctcctccagg gccacataga tgtggtacag ggaggccatc accagcttga 
     2041 agccgtctcg ggtcacctgg cccttctgaa agttcctcat gaactcagca ttctctgcct 
     2101 gggtgtgcac ctccttggtg gcctccttca gggcctctga caaatcctgg ggcatgctgt 
     2161 cgggttgcgg acgctccatc cggccggtgc tgggctcgtt cgtgctggct cagagtnnng 
     2221 aattcctgca gcccggggga tccactagtt ctagagcggc cgccaccgcg gtggagctcc 
     2281 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg 
     2341 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     2401 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     2461 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     2521 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     2581 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     2641 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     2701 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     2761 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     2821 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     2881 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 
     2941 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     3001 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     3061 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     3121 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     3181 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     3241 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     3301 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     3361 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     3421 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     3481 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     3541 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     3601 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     3661 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     3721 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     3781 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     3841 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     3901 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     3961 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     4021 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     4081 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     4141 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     4201 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     4261 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     4321 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     4381 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     4441 caaatagggg ttccgcgcac atttccccga aaagtgccac 
//
