LOCUS       pBLHIL6CAT              5422 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2861.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBLHIL6CAT|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBLHIL6CAT" 0 5422 
COMMENT       (CDBMol 0 0 1 1 1 0 0 26714168 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
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COMMENT            1 1 1 1 "32..672") 
COMMENT        #4=(CFSignal (CObList) "hIL6" 30 0 0 673 1208 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "673..1208") 
COMMENT        #5=(CFSignal (CObList) "cat" 4 0 0 1260 1919 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1260..1919") 
COMMENT        #6=(CFSignal (CObList) "(t)" 4 0 0 2009 2083 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "INT" 15 0 0 2084 2150 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2084..2150") 
COMMENT        #8=(CFSignal (CObList) "3UTR" 21 0 0 2151 2702 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2151..2702") 
COMMENT        #9=(CFSignal (CObList) "polyA" 43 0 0 2703 2799 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2703..2799") 
COMMENT        #10=(CFSignal (CObList) "polyA" 43 0 1 2703 2799 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2703..2799)") 
COMMENT        #11=(CFSignal (CObList) "lac" 30 0 1 2938 3021 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2938..3021)") 
COMMENT        #12=(CFSignal (CObList) "ORI" 33 0 1 3113 3515 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3113..3515)") 
COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 4056 4916 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4056..4916)") 
COMMENT        #14=(CFSignal (CObList) "polyA" 43 0 0 5048 5122 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5048..5122") 
COMMENT        #15=(CFSignal (CObList) "polyA" 43 0 1 5048 5122 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5048..5122)") 
COMMENT        #16=(CFSignal (CObList) "polyA" 43 0 0 5263 5359 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5263..5359") 
COMMENT        #17=(CFSignal (CObList) "polyA" 43 0 1 5263 5359 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5263..5359)")) (CObList) 
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COMMENT                          "DNA 'pBLHIL6CAT'" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5422 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Start: 1260  End: 1919" 1) 
COMMENT                                   #36=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "chloramphenicol acetyltransferase coding sequence" 
COMMENT                                        1) 
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COMMENT                                   #38=(CLinePar 
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COMMENT                                        "Start: 2009  End: 2083" 1) 
COMMENT                                   #41=(CLinePar 
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COMMENT                                        "part of the SV40 small t-antigen" 1) 
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COMMENT                                        "Start: 4056  End: 4916 (Complementary)" 
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COMMENT                                   #46=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
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COMMENT                                   #48=(CLinePar 
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COMMENT                                        "   complement(4056..4916)" 1))) "AMP"))) 
COMMENT                          "CDS (3 total)") 
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COMMENT                                        "Start: 2084  End: 2150" 1) 
COMMENT                                   #52=(CLinePar 
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COMMENT                                        "intron of the SV40 small t-antigen" 1) 
COMMENT                                   #53=(CLinePar 
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COMMENT                                        "Start: 32    End: 672 " 1) 
COMMENT                                   #58=(CLinePar 
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COMMENT                                        "human interleukin 6 promoter" 1) 
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COMMENT                                        "Start: 2938  End: 3021 (Complementary)" 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                          "Replication Origin (1 total)") 
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FEATURES             Location/Qualifiers
     misc_feature    32..672
                     /vntifkey="21"
                     /label=5FR
                     /note="5' flanking region of the human interleukin 6 gene"
     promoter        673..1208
                     /vntifkey="30"
                     /label=hIL6
                     /note="human interleukin 6 promoter"
     CDS             1260..1919
                     /vntifkey="4"
                     /label=cat
                     /note="chloramphenicol acetyltransferase coding sequence"
     CDS             2009..2083
                     /vntifkey="4"
                     /label=(t)
                     /note="part of the SV40 small t-antigen"
     intron          2084..2150
                     /vntifkey="15"
                     /label=INT
                     /note="intron of the SV40 small t-antigen"
     misc_feature    2151..2702
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the SV40 small t-antigen"
     terminator      2703..2799
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2703..2799)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     promoter        complement(2938..3021)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(3113..3515)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(4056..4916)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     terminator      5048..5122
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5048..5122)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     terminator      5263..5359
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     terminator      complement(5263..5359)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
BASE COUNT     1503 a      1236 c      1203 g      1480 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatcctcct gcaagagaca ccatcctgag 
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat 
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 
     1201 tgaggctcat tctgccctcg aggagcttgg cgagattttc aggagctaag gaagctaaaa 
     1261 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac 
     1321 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata 
     1381 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc 
     1441 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg 
     1501 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa 
     1561 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt 
     1621 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga 
     1681 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg 
     1741 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg 
     1801 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg 
     1861 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat 
     1921 ttttttaagg cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa 
     1981 taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac ttctgtggtg 
     2041 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt 
     2101 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct 
     2161 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct 
     2221 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc 
     2281 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt 
     2341 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg 
     2401 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta 
     2461 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt 
     2521 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg 
     2581 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca 
     2641 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 
     2701 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 
     2761 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 
     2821 ttgtccaaac tcatcaatgt atcttatcat gtctggatcg atccccgggt accgagctcg 
     2881 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca 
     2941 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa 
     3001 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 
     3061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 
     3121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 
     3181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 
     3241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 
     3301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 
     3361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 
     3421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 
     3481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 
     3541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 
     3601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 
     3661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 
     3721 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 
     3781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 
     3841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 
     3901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 
     3961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 
     4021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 
     4081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 
     4141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 
     4201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 
     4261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 
     4321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 
     4381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 
     4441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 
     4501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 
     4561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 
     4621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 
     4681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 
     4741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 
     4801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 
     4861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 
     4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 
     4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 
     5041 ccacctgaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 
     5101 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 
     5161 tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc 
     5221 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 
     5281 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 
     5341 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 
     5401 atcttatcat gtctggatct cg 
//
