ID pPNEcad standard; circular DNA; ; 8300 BP. DT 31-OCT-2007 DE LMBP 2828 CC Accession number: LMBP 2828 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 5-NOV-2007 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT terminator complement(402..853) FT /vntifkey="43" FT /label=3'\UTR\of\mPGK1,\incl.\polyA FT /note="3' UTR of the mouse PGK1 gene, incl. polyA" FT misc_feature complement(912..945) FT /vntifkey="21" FT /label=3'\UTR\of\mE-cad FT /note="3' UTR of the mouse E-cadherin gene" FT CDS complement(946..3600) FT /vntifkey="4" FT /label=mE-cad FT /note="mouse E-cadherin coding sequence" FT misc_feature complement(3601..3633) FT /vntifkey="21" FT /label=5'\UTR\of\mE-cad FT /note="5' UTR of the mouse E-cadherin gene" FT promoter complement(3660..4167) FT /vntifkey="29" FT /label=mPGK1\promoter FT /note="mouse phosphoglycerate kinase 1 promoter" FT terminator complement(4203..4654) FT /vntifkey="43" FT /label=3'\UTR\of\mPGK1,\incl.\polyA FT /note="3' UTR of the mouse PGK1 gene, incl. polyA" FT CDS complement(4679..5473) FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT promoter complement(5486..5993) FT /vntifkey="29" FT /label=mPGK1\promoter FT /note="mouse phosphoglycerate kinase 1 promoter" FT promoter complement(6053..6069) FT /vntifkey="30" FT /label=T3\promoter FT /note="phage T3 promoter" FT promoter complement(6133..6216) FT /vntifkey="30" FT /label=lac\promoter FT /note="E. coli lac operon promoter" FT rep_origin complement(6308..6699) FT /vntifkey="33" FT /label=ColE1\ori FT /note="origin plasmid" FT CDS complement(7240..8100) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 8300 BP; 1937 A; 2209 C; 2231 G; 1922 t; 1 other; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac 420 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctn 480 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca 540 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat 600 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta 660 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca 720 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt 780 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga 840 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata 900 caaaacaaaa gccatggtgc cacacggggg agacttgcta gtcccctagt cgtcctcgcc 960 accgccgtac atgtccgcca gcttcttgaa tcggttgccc cactcgttca gataatcgta 1020 gtcctggtcc tgatccgact cagaggagtt cagtgagctc aggctagcgg cttcagaacc 1080 actgccctcg taatcgaaca ccaacagaga gtcgtaaggg ggtgccgtgg ggtcgctgtc 1140 ggctgccttc aggttttcat cgatgaagtt tccaatttca tcaggattgg caggacgggg 1200 acgatactgg ggcacgctca tgagggtggg agccacatca tttcgagtca cttccggtcg 1260 ggcatccagg cccctgtgca gctggctcaa atcaaagtcc tggtcttctt ctccacctcc 1320 ttcttcatca tagtaataca cattgtcccg ggtatcatca tctggtggca gcaggggctc 1380 tttgaccacc gttctcctcc gtagaaacag taggagcagc aggatcagaa tcagcagggc 1440 gaggatccct ccaaggattc cgaggatggc aggaacttgc aatcctgctg ccacgattcc 1500 cgccttcatg cagttgttga ccgtcccttc acagtcacac acatggacgt ccaacgtggt 1560 cacctggtct ttgttctggt tatccgcgag cttgagatgg attttgtatt cgccaatctc 1620 taagtccttt cttggttgca aaatgagaga ttcttgagct gcgtcattat actcaatggt 1680 ccagttgacg ctggccccat gggttagctc agcagtaaag ggggacgtgt tggggggaag 1740 gtctggatcc aagatggtga tgatatgagg ctgtgggttc ctctggcaga actgcatgtt 1800 tcgaggttct gggatgggag cgttgtcatt gacgtctaac aggaccagga gaagagtgcc 1860 cgtgccagtg gcaatgggtg aaccatcatc tgtggcgatg atgagagcta catatgtgct 1920 gttcttcaca tgctcagcgt cttctctgtc catctcagcg cgcgtgaaaa tggcaccagt 1980 ctctgggtta atctccagcc agttggcagt gtccctccaa atccgatacg tgatcttctg 2040 atccatgaac gtgtccggct ctcgagcggt ataagatgtg atttcctgac ccacaccaaa 2100 gtcttcgggc acttcgactc tcctctccgc aggcataaag atgggggctt cattcacgtc 2160 taccacgtcc acagtgacag tggctgtgga agggacaaga gacccctcaa agggttcctc 2220 gttctccact ctcacatgaa ggatgtattg ctgcttggcc tcaaaatcca agcccttggc 2280 tgttttcaaa atgccatcat tggtcgtggg gtctgtgacg acaacgaact gctggtcagg 2340 atcgttgact acggtgtaca cagctttcca cgccggagtg ttgggggcat catcatcggt 2400 cactttgagt gtggcgatcc gggcattgac ctcattctca ggcacttgac cctgatacgt 2460 gctcgggttg aagacaggag cgttgtcatt aatatccttg acagtgatca cagccttggc 2520 tgttgtgctc aagccttcgc cttgaaggtc agcagcctga accaccagag tgtatgtagg 2580 gtaactctct cggtccagcc cagaggtgag cacactgatg accccggtgt ccctattgac 2640 agtgaacatg tttttgtgag gcagctcagg atcctggctg acgatggtgt aggcgatggc 2700 agcgttgtag gtgttgacgt catcgtctgc atcggtggct gagaccttca tcacggaggt 2760 tcctggaaca gcgccttctg caacggatcc ctcaaacacc tcctgggtaa actctggcct 2820 gttgtcattc tgatctgtca ctgtgatcac tatctccatg ggatcctcca ccgcttcccc 2880 atttgatgac acggcatgag aatagaggat gtacttggca atggcttctc tatccagagg 2940 ctgtgtcact ttcagccagc ctgtctccct ctcaatgatg aaaacgccaa cggggggttt 3000 gtcagctcct tggccggtga tgctgtagaa aacctttgtt tctttgtccc tgttggattt 3060 gatctgaacc aggttctttg ggaattcacc cttttcattt tcggggcagc tgatgggagg 3120 gatgacccag tctcgtttct gtcttctgag acctgggtac acgctgggaa acatgagcag 3180 ctctgggttg gattcagagg cagggtcgcg gtggtggtgc cggtgatggt ggtgccccat 3240 ggacttcagc gtcactttgg tagacagctc cctatgactg gagtcccggg cgcggacgag 3300 gaaactggtc tccagcttgt ggagctttag atgccgcttc actgtgatgg tgccgtctgt 3360 cgccactttg aatcgggagt cttccgaaaa gaaggctgtc cttggccggc cggtgcatcc 3420 ttcaaatctc actctgccca ggacatggcc tctctccagg tgcctctccg gcaccgggaa 3480 ggtgtagacc tcggaactga agccgggact gcaggactca ggctccagct cctggcaaag 3540 ccatgaggag acctgcagca ggagcaggag cgcggaaaag ctgcggcacc gggctcccat 3600 ggcggacccg gcagacgccg agcaaacact gaggacgctg ttaagcgggt cgctgcaggt 3660 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt 3720 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct 3780 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag 3840 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat 3900 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg 3960 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc 4020 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc 4080 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg 4140 gaaaagcgcc tcccctaccc ggtagaattc gagctcggta cccggggatc ctctagagtc 4200 agcttctgat ggaattagaa cttggcaaaa caatactgag aatgaagtgt atgtggaaca 4260 gaggctgctg atctcgttct tcaggctatg aaactgacac atttggaaac cacagtactt 4320 agaaccacaa agtgggaatc aagagaaaaa caatgatccc acgagagatc tatagatcta 4380 tagatcatga gtgggaggaa tgagctggcc cttaatttgg ttttgcttgt ttaaattatg 4440 atatccaact atgaaacatt atcataaagc aatagtaaag agccttcagt aaagagcagg 4500 catttatcta atcccacccc acccccaccc ccgtagctcc aatccttcca ttcaaaatgt 4560 aggtactctg ttctcaccct tcttaacaaa gtatgacagg aaaaacttcc attttagtgg 4620 acatctttat tgtttaatag atcatcaatt tctgcagact tacagcggat cgatcccctc 4680 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac 4740 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg 4800 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc 4860 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga 4920 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga 4980 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac 5040 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg 5100 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag 5160 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag 5220 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg 5280 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg 5340 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg 5400 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat 5460 cggccgatcc catattggct gcaggtcgaa aggcccggag atgaggaaga ggagaacagc 5520 gcggcagacg tgcgcttttg aagcgtgcag aatgccgggc ctccggagga ccttcgggcg 5580 cccgccccgc ccctgagccc gcccctgagc ccgcccccgg acccacccct tcccagcctc 5640 tgagcccaga aagcgaagga gcaaagctgc tattggccgc tgccccaaag gcctacccgc 5700 ttccattgct cagcggtgct gtccatctgc acgagactag tgagacgtgc tacttccatt 5760 tgtcacgtcc tgcacgacgc gagctgcggg gcggggggga acttcctgac taggggagga 5820 gtagaaggtg gcgcgaaggg gccaccaaag aacggagccg gttggcgcct accggtggat 5880 gtggaatgtg tgcgaggcca gaggccactt gtgtagcgcc aagtgcccag cggggctgct 5940 aaagcgcatg ctccagactg ccttgggaaa agcgcctccc ctacccggta gaattgacct 6000 gcaggggccc tcgagcggcc gccaccgcgg tggagctcca gcttttgttc cctttagtga 6060 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat 6120 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc 6180 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 6240 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 6300 attgggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg 6360 cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag 6420 gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccacgt agcaggcgtt 6480 tttccatagg ctccgccccc ctgacgagca tcacaaaaat agacgctcaa gtcagaggtg 6540 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6600 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6660 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6720 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6780 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6840 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6900 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6960 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7020 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7080 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7140 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7200 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7260 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7320 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7380 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7440 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7500 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 7560 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7620 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7680 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7740 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7800 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 7860 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7920 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7980 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8040 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8100 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8160 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8220 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8280 tatcacgagg ccctttcgtc 8300 //