LOCUS       pPNT                    7331 bp    DNA     circular     24-OCT-2008
DEFINITION  LMBP 2682.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2682
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 24-OCT-2008
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|427719696|
COMMENT     VNTDBDATE|497029360|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPNT|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTOAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT        #2=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 2942 0 4768 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "FspI" "tgcgca" 4 0 3 259 0 4274 0 6567 0 0 "")) 
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COMMENT        #5=(CFSignal (CObList) "ColE1 ori" 33 0 1 5339 5730 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 5164 5247 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "T3 promoter" 30 0 1 5084 5100 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "mPGK1 promoter" 29 0 1 4517 5024 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 1 3710 4504 0 
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COMMENT        #10=(CFSignal (CObList) "3' UTR of mPGK1, incl. polyA" 43 0 1 3234 3685 
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COMMENT                          "DNA Plasmid 'pPNT'" 1) 
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COMMENT                          "Currently local object. Original author: LMBP Plasmid Collection" 
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COMMENT                          "Created: 11/22/06 11:01" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6271..7131)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(5339..5730)
                     /vntifkey="33"
                     /label=ColE1\ori
     promoter        complement(5164..5247)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        complement(5084..5100)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(4517..5024)
                     /vntifkey="29"
                     /label=mPGK1\promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             complement(3710..4504)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      complement(3234..3685)
                     /vntifkey="43"
                     /label=3'\UTR\of\mPGK1,\incl.\polyA
     promoter        complement(2691..3198)
                     /vntifkey="29"
                     /label=mPGK1\promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             complement(1463..2593)
                     /vntifkey="4"
                     /label=HSV-TK
                     /note="Herpes simplex thymidine kinase coding sequence"
     terminator      complement(860..1462)
                     /vntifkey="43"
                     /label=3'\UTR\of\HSV-TK,\incl.\polyA
     terminator      complement(402..853)
                     /vntifkey="43"
                     /label=3'\UTR\of\mPGK1,\incl.\polyA
BASE COUNT     1700 a      2014 c      2050 g      1567 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgatg gaattagaac 
      421 ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt 
      481 caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca 
      541 agagaaaaac aatgatccca cgagagatct atagatctat agatcatgag tgggaggaat 
      601 gagctggccc ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta 
      661 tcataaagca atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca 
      721 cccccacccc cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt 
      781 cttaacaaag tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga 
      841 tcatcaattt ctgcaggtcc tggccggggt cccgcggaaa ctcggccgtg gtgaccaata 
      901 caaaacaaaa gcgctcctcg taccagcgaa gaaggggcag agatgccgta gtcaggttta 
      961 gttcgtccgg cggcgccaga aatccgcgcg gtggtttttg ggggtcgggg gtgtttggca 
     1021 gccacagacg cccggtgttc gtgtcgcgcc agtacatgcg gtccatgccc aggccatcca 
     1081 aaaaccatgg gtctgtctgc tcagtccagt cgtggacctg accccacgca acgcccaaaa 
     1141 gaataacccc cacgaaccat aaaccattcc ccatggggga ccccgtccct aacccacggg 
     1201 gcccgtggct atggcgggct tgccgccccg acgttggctg cgagccctgg gccttcaccc 
     1261 gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt 
     1321 ctcggtgggg tatcgacaga gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac 
     1381 gacccaacac ccgtgcgttt tattctgtct ttttattgcc gtcatagcgc gggttccttc 
     1441 cggtattgtc tccttccgtg tttcagttag cctcccccat ctcccgggca aacgtgcgcg 
     1501 ccaggtcgca gatcgtcggt atggagcctg gggtggtgac gtgggtctgg accatcccgg 
     1561 aggtaagttg cagcagggcg tcccggcagc cggcgggcga ttggtcgtaa tccaggataa 
     1621 agacgtgcat gggacggagg cgtttggcca agacgtccaa ggcccaggca aacacgttat 
     1681 acaggtcgcc gttgggggcc agcaactcgg gggcccgaaa cagggtaaat aacgtgtccc 
     1741 cgatatgggg tcgtgggccc gcgttgctct ggggctcggc accctggggc ggcacggccg 
     1801 tccccgaaag ctgtccccaa tcctcccacc acgacccgcc gccctgcaga taccgcaccg 
     1861 tattggcaag cagcccgtaa acgcggcgaa tcgcggccag catagccagg tcaagccgct 
     1921 cgccggggcg ctggcgtttg gccaggcggt cgatgtgtct gtcctccgga agggccccca 
     1981 acacgatgtt tgtgccgggc aaggtcggcg ggatgagggc cacgaacgcc agcacggcct 
     2041 ggggggtcat gctgcccata aggtatcgcg cggccgggta gcacaggagg gcggcgatgg 
     2101 gatggcggtc gaagatgagg gtgagggccg ggggcggggc atttgagctc ccagcctccc 
     2161 ccccgatatg aggagccaga acggcgtcgg tcacggcata aggcatgccc attgttatct 
     2221 gggcgcttgt cattaccacc gccgcgtccc cggccgatat ctcaccctgg tcgaggcggt 
     2281 gttgtgtggt gtagatgttc gcgattgtct cggaagcccc cagcacctgc cagtaagtca 
     2341 tcggctcggg tacgtagacg atatcgtcgc gcgaacccag ggccaccagc agttgcgtgg 
     2401 tggtggtttt ccccatcccg tgaggaccgt ctatataaac ccgcagtagc gtgggcattt 
     2461 tctgctccag gcggacttcc gtggcttctt gctgccggcg agggcgcaac gccgtacgtc 
     2521 ggttgctatg gccgcgagaa cgcgcagcct ggtcgaacgc agacgcgtgt tgatggcagg 
     2581 ggtacgaagc catacgcgct tctacaaggc gcttgccaaa gaggtgcggg agtttcacgc 
     2641 caccaagatc tgcggcacgc tgttgacgct gttaagcggg tcgctgcagg tcgaaaggcc 
     2701 cggagatgag gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc 
     2761 cgggcctccg gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc 
     2821 cccggaccca ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg 
     2881 gccgctgccc caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag 
     2941 actagtgaga cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg 
     3001 ggggaacttc ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg 
     3061 agccggttgg cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta 
     3121 gcgccaagtg cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc 
     3181 ctcccctacc cggtagaatt cgagctcggt acccggggat cctctagagt cagcttctga 
     3241 tggaattaga acttggcaaa acaatactga gaatgaagtg tatgtggaac agaggctgct 
     3301 gatctcgttc ttcaggctat gaaactgaca catttggaaa ccacagtact tagaaccaca 
     3361 aagtgggaat caagagaaaa acaatgatcc cacgagagat ctatagatct atagatcatg 
     3421 agtgggagga atgagctggc ccttaatttg gttttgcttg tttaaattat gatatccaac 
     3481 tatgaaacat tatcataaag caatagtaaa gagccttcag taaagagcag gcatttatct 
     3541 aatcccaccc cacccccacc cccgtagctc caatccttcc attcaaaatg taggtactct 
     3601 gttctcaccc ttcttaacaa agtatgacag gaaaaacttc cattttagtg gacatcttta 
     3661 ttgtttaata gatcatcaat ttctgcagac ttacagcgga tcgatcccct cagaagaact 
     3721 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca 
     3781 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg 
     3841 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc 
     3901 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct 
     3961 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat 
     4021 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct 
     4081 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc 
     4141 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga 
     4201 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt 
     4261 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt 
     4321 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct 
     4381 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat 
     4441 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcggccgatc 
     4501 ccatattggc tgcaggtcga aaggcccgga gatgaggaag aggagaacag cgcggcagac 
     4561 gtgcgctttt gaagcgtgca gaatgccggg cctccggagg accttcgggc gcccgccccg 
     4621 cccctgagcc cgcccctgag cccgcccccg gacccacccc ttcccagcct ctgagcccag 
     4681 aaagcgaagg agcaaagctg ctattggccg ctgccccaaa ggcctacccg cttccattgc 
     4741 tcagcggtgc tgtccatctg cacgagacta gtgagacgtg ctacttccat ttgtcacgtc 
     4801 ctgcacgacg cgagctgcgg ggcggggggg aacttcctga ctaggggagg agtagaaggt 
     4861 ggcgcgaagg ggccaccaaa gaacggagcc ggttggcgcc taccggtgga tgtggaatgt 
     4921 gtgcgaggcc agaggccact tgtgtagcgc caagtgccca gcggggctgc taaagcgcat 
     4981 gctccagact gccttgggaa aagcgcctcc cctacccggt agaattgacc tgcaggggcc 
     5041 ctcgagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt 
     5101 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     5161 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     5221 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     5281 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     5341 ttttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggta 
     5401 ctgactcaca caaaaacggt aacacagtta tccacagaat caggggataa ggccggaaag 
     5461 aacatgtgag caaaagacca ggaacaggaa gaaggccacg tagcaggcgt ttttccatag 
     5521 gctccgcccc cctgacgagc atcacaaaaa tagacgctca agtcagaggt ggcgaaaccc 
     5581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 
     5641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 
     5701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 
     5761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 
     5821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 
     5881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 
     5941 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 
     6001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 
     6061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 
     6121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 
     6181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 
     6241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 
     6301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 
     6361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 
     6421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 
     6481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 
     6541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 
     6601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 
     6661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 
     6721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 
     6781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 
     6841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 
     6901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 
     6961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 
     7021 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 
     7081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 
     7141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 
     7201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 
     7261 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag 
     7321 gccctttcgt c 
//
