LOCUS       pLPPHIs                 7373 bp    DNA     circular     26-AUG-2014
DEFINITION  LMBP 2587.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|707306855|
COMMENT     VNTDBDATE|707306855|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLPPHIs|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT            1 1 1 1 "") 
COMMENT        #1=(CFSignal (CObList) "lacUV5" 30 0 0 235 291 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #2=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 292 329 0 
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COMMENT        #6=(CFSignal (CObList) "lacIq" 30 0 0 1669 1746 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "lppT" 43 0 0 901 966 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "lacI" 4 0 0 1747 2829 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
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COMMENT                          "DNA Plasmid 'pLPPHIs'" 1) 
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COMMENT                          "Created: 08/26/14 10:07" 1) 
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COMMENT                          "Last Modified: 08/26/14 10:07" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7373 bp" 1) 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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COMMENT                                  5.6268 13.9386 0 -10 3.09707 1.17389 #116#) 
COMMENT                            #118=(CWideArrow 
COMMENT                                  (CWideLine 
COMMENT                                   (CWidget 0 (3 #6# 0) 1 2 0 0 #73# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 (LOGBRUSH 0 128 0) 1 4.78763 
COMMENT                                   4.854 1 0.082322) 0.8 1.8 0) 
COMMENT                            #119=(CLabel (CWidget 0 (0 0) 1 2 0 0 #57# 1 100) 
COMMENT                                  (LOGPEN 0 0 0) 1 
COMMENT                                  (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                   "Arial") 1.74233 0.666667 0 "lacIq" "@N" 1 
COMMENT                                  14.409 1.74233 0 -10 2.17295 1.17389 #118#)) 
COMMENT                           (CObjectList)) 
COMMENT                     #120=(CGroupWidget 
COMMENT                           (CWidget 32 (7 32 0) 1 2 0 0 Nil -674 100) 
COMMENT                           (CObjectList 
COMMENT                            #121=(CScratch 
COMMENT                                  (CWidget 0 (3 #2# 0) 1 2 0 0 #77# 245 100) 
COMMENT                                  (LOGPEN 0 6 16711680) 8 1 6.02559 1.9 0.082322 
COMMENT                                  1) 
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COMMENT                                  (LOGPEN 0 0 0) 1 
COMMENT                                  (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                   "Arial") 1.74233 0.666667 0 
COMMENT                                  "5' UTR of lacZ, incl. RBS" "@N" 1 11.3782 
COMMENT                                  12.1963 0 -10 10.4152 1.17389 #121#)) 
COMMENT                           (CObjectList)) 
COMMENT                     #123=(CGroupWidget 
COMMENT                           (CWidget 33 (7 33 0) 1 2 0 0 Nil 686 100) 
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COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #60# 0 100) 
COMMENT                                   (LOGPEN 0 0 3355545) 1 (LOGBRUSH 0 6724095 0) 
COMMENT                                   1 1.36559 1.70847 1 0.082322) 0.8 1.8 1) 
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COMMENT                                  (LOGPEN 0 0 0) 1 
COMMENT                                  (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                   "Arial") 1.74233 0.666667 0 "ORI" "@N" 1 
COMMENT                                  -14.2938 0 0 -10 1.59849 1.17389 #124#)) 
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COMMENT                     #126=(CGroupWidget 
COMMENT                           (CWidget 43 (7 43 0) 1 2 0 0 Nil -116 100) 
COMMENT                           (CObjectList 
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COMMENT                                   100) (LOGPEN 0 8 13395507) 10 0.835356 
COMMENT                                  5.45128 5.50744) 
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COMMENT                                  (LOGPEN 0 0 0) 1 
COMMENT                                  (LOGFONT 46 17 0 0 700 0 0 0 0 3 2 1 34 
COMMENT                                   "Arial") 1.74233 0.666667 0 "lppT" "@N" 1 
COMMENT                                  13.5186 5.22699 0 -10 2.09802 1.17389 #127#)) 
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COMMENT              #129=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -953 100) 
COMMENT                    (CObjectList) (CObjectList)) 
COMMENT              #130=(CGroupWidget (CWidget 11 (0 0) 1 2 0 0 Nil -465 100) 
COMMENT                    (CObjectList) (CObjectList)) 
COMMENT              #131=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -395 100) 
COMMENT                    (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 1196705607 
COMMENT       (CStringList) 1094795585 1195852099 (CObList)))
FEATURES             Location/Qualifiers
     promoter        24..208
                     /vntifkey="30"
                     /label=lpp
     promoter        235..291
                     /vntifkey="30"
                     /label=lacUV5
     RBS             292..329
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     CDS             350..412
                     /vntifkey="4"
                     /label=sompA
     CDS             413..598
                     /vntifkey="4"
                     /label=H1-4
     CDS             599..740
                     /vntifkey="4"
                     /label=(CH2)
     terminator      901..966
                     /vntifkey="43"
                     /label=lppT
     promoter        1669..1746
                     /vntifkey="30"
                     /label=lacIq
     CDS             1747..2829
                     /vntifkey="4"
                     /label=lacI
     rep_origin      complement(5366..5768)
                     /vntifkey="33"
                     /label=ORI
     CDS             complement(6309..7169)
                     /vntifkey="4"
                     /label=AMP
BASE COUNT     1752 a      2003 c      1862 g      1754 t         2 others
ORIGIN
        1 ataaccagaa gcaataaaaa atcaaatcgg atttcactat ataatctcac tttatctaag 
       61 atgaatccga tggaagcatc ctgttttctc tcaatttttt tatctaaaac ccagcgttcg 
      121 atgcttcttt gagcgaacga tcaaaaataa gtgccttccc atcaaaaaaa tattctcaac 
      181 ataaaaaact ttgtgtaata cttgtaacgc tacatggaga ttaactcaat ctagcactca 
      241 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gaattgtgag 
      301 cggataacaa tttcacacag gaaacagctc tagataacga gggcaaaaaa tgaaaaagac 
      361 agctatcgcg attgcagtgg cactggctgg tttcgctacc gtagcgcagg ccgagctcaa 
      421 aaccccactt ggtgacacaa ctcacacatg cccacggtgc ccagagccca aatcttgtga 
      481 cacacctccc ccgtgcccac ggtgcccaga gcccaaatct tgtgacacac ctcccccatg 
      541 cccacggtgc ccagagccca aatcttgtga cacacctccc ccgtgcccaa ggtgcccagg 
      601 gccctaactc ttgggaggac cgtcagtctt cctcttcccc ccaaaaccca aggataccct 
      661 tatgatttcc cggacccctg aggtcacgtg cgtggtggtg gacgtgagcc acgaagaccc 
      721 cgaggtccag ttcaagtggt ctcagggccc gtttactcat atatacttta gattgattta 
      781 gcttggatcc ggctgagcaa cgacgtgaac gcaatgcgtt ccgacgttca ggctgctaaa 
      841 gatgacgcag ctcgtgctaa ccagcgtctg gacaacatgg ctactaaata ccgcaagtaa 
      901 tagtacctgt gaagtgaaaa atggcgcaca ttgtgcgaca ttttttttgt ctgccgttta 
      961 ccgctactgc gtcacgcgta acatattccc ttgctctggt tcaccattct gcgctgactc 
     1021 tactgaaggc gcattgctgg ctgcgggagt tgctccactg ctcaccgaaa ccggataccc 
     1081 tgcccgacga tacaacgctt tatcgactaa cttctgatct acagccttat tgtctttaaa 
     1141 ttgcgtaaag cctgctggca gtgtgtatgg cattgtctga acgttctgct gttcttctgc 
     1201 cgatagtggt cgatgtactt caacataacg catcccgtta ggctccacgg aatatttcac 
     1261 cggttcgttg atcactttca ccggcgttcc cgtccgcacg ctggagaaca aggctttaat 
     1321 atccggtgca ttcatgcgaa tacaccctga actgacgcgc aaaccgacgc tgtccggcgc 
     1381 actggtacca tgaatgaggt attcgccatt accatgcgcg aggcgcagtg cgtaacgtcc 
     1441 tagcgggtta tttggtccgg caggaacgac tggcggtaat ttaatgccac gctccagcga 
     1501 acgctgacga atgcctgccg taggcgtcca ggttgggtta gggattttct gcccaacacg 
     1561 cgtttccatc accggcgttt ccagcccctg caatccaata cctattggat aaacctgcac 
     1621 aatattttct cccggcggat aataataaag gcgcagctct gcaaggttga caccatcgaa 
     1681 tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg 
     1741 gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag 
     1801 accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg 
     1861 gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc 
     1921 aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa 
     1981 attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg 
     2041 gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc 
     2101 gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct 
     2161 gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt 
     2221 attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt 
     2281 caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg 
     2341 gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc 
     2401 gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt 
     2461 cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc 
     2521 gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac 
     2581 agctcatgtt atatcccgcc gtcaaccacc atcaaacagg attttcgcct gctggggcaa 
     2641 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg 
     2701 ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct 
     2761 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 
     2821 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 
     2881 acactttatg ctaacgataa tcccctgacg cggtgcatca ggtaataaca gttgtgaagg 
     2941 aatagttatc gtcgtaccag gttttggcac cggggcgata gtgttattgg cttcaaggat 
     3001 caacattgcc gcagtatcaa aacgtcgggc aatagcctga aggtttttat ccccttcttg 
     3061 caccgtatac gtttgatttt gcccaaccag tcggcttccg gttggtggta gcggataatc 
     3121 aaccgcccag gcagcctgga tggcgctaaa agcgccgata agcgtgagtg taagcaaaga 
     3181 cgcgcgtttc attgtaaacc tcctgtattt gccggagact cacgctgaaa cgtcggatgg 
     3241 cgcttatgtt cacctgaaac caaaacactc ctgtnncagg tcagtgtaaa cattgaccat 
     3301 ccggcaatgt tgagccaacc ggatgaaagc tgtcctttta gtttagctaa gtgcagcggc 
     3361 tttggcgcga attgcgcgaa tcatcgcttc cagaccttgt gaacgagatg gggtgagatg 
     3421 ttgggtgagc gccatttttt caaaccacgg acgcacatcg aaattgacaa tatcctgcgg 
     3481 cgtcatctga tcgtagagaa taaagacgaa cgcaataagc cctttcacaa tcgccgcatc 
     3541 gctgtcgccc tgtaattcaa taattccctg ggcattctgg cgcatgacaa tccacacctg 
     3601 actctgacag ccctgaatgc tattttgtgg acttctgtct tcgtcgcgta attctggcag 
     3661 acgctggggg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc 
     3721 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac 
     3781 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga 
     3841 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca 
     3901 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg 
     3961 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta 
     4021 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc 
     4081 aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa 
     4141 cttcgatcac tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga 
     4201 acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc 
     4261 gcggtgcatg gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg 
     4321 attcaccact ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac 
     4381 caacccttgg cagaacatat ccatcgcgtc cgccatctcc agcagccgca cgcggcgcat 
     4441 ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac 
     4501 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg 
     4561 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt 
     4621 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt 
     4681 ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat ctgtattaac 
     4741 gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc ataccgccag 
     4801 ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa cccgtatcgt 
     4861 gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc ccccttacac 
     4921 ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag 
     4981 ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat 
     5041 ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg 
     5101 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 
     5161 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 
     5221 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 
     5281 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 
     5341 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     5401 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     5461 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     5521 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     5581 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     5641 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     5701 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     5761 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     5821 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     5881 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     5941 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 
     6001 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     6061 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     6121 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     6181 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     6241 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     6301 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     6361 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     6421 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     6481 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     6541 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     6601 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 
     6661 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     6721 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     6781 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     6841 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     6901 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 
     6961 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     7021 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     7081 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     7141 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     7201 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     7261 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 
     7321 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tca 
//
