LOCUS       pPLcA1                  3198 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 2469.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2469
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|461507069|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPLcA1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPLcA1" 0 3198 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
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COMMENT             "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT             "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0)) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #1=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 476 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "BglII" "agatct" 2 0 1 654 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 130 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 1 2168 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "HBc" 4 0 0 131 682 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "131..682") 
COMMENT        #6=(CFSignal (CObList) "synthetic polyA" 43 0 0 686 725 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "691..717") 
COMMENT        #7=(CFSignal (CObList) "ampicillin" 4 0 1 1724 2584 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1724..2584)") 
COMMENT        #8=(CFSignal (CObList) "lambda PL promoter" 30 0 0 2950 3080 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2950..3080") 
COMMENT        #9=(CFSignal (CObList) "5' UTR of MS2 polymerase, incl. RBS" 32 0 0 98 
COMMENT            130 0 (CStringList) (CStringList) 1 1 1 1 "98..130") 
COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 781 1183 0 (CStringList) 
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COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #12=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pPLcA1'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modified: 10/29/07 12:24" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 3198 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "phage lambda major leftward promoter" 1) 
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FEATURES             Location/Qualifiers
     CDS             131..682
                     /vntifkey="4"
                     /label=HBc
     terminator      686..725
                     /vntifkey="43"
                     /label=synthetic\polyA
                     /note="synthetic polyA signal"
     CDS             complement(1724..2584)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     promoter        2950..3080
                     /vntifkey="30"
                     /label=lambda\PL\promoter
                     /note="phage lambda major leftward promoter"
     RBS             98..130
                     /vntifkey="32"
                     /label=5'\UTR\of\MS2\polymerase,\incl.\RBS
                     /note="5' UTR of the MS2 polymerase gene, incl. RBS"
     rep_origin      complement(781..1183)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
BASE COUNT      808 a       819 c       773 g       798 t 
ORIGIN
        1 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat 
       61 tccctcagca atcgcagcaa actccggcat ctactaatag acgccggcca ttcaaacatg 
      121 aggattaccc atggatatcg atccttataa agaattcgga gctactgtgg agttactctc 
      181 gtttctcccg agtgacttct ttccttcagt acgagacctt ctggataccg ccagcgcgct 
      241 gtatcgggaa gccttggagt ctcctgagca ctgcagccct caccatactg ccctcaggca 
      301 agcaattctt tgctgggggg agctcatgac tctggccacg tgggtgggtg ttaacttgga 
      361 agatccagct agcagggacc tggtagtcag ttatgtcaac actaatatgg gtttaaagtt 
      421 caggcaactc ttgtggtttc acattagctg cctcactttc ggccgagaaa cagttctaga 
      481 atatttggtg tctttcggag tgtggatccg cactcctcca gcttataggc ctccgaatgc 
      541 ccctatcctg tcgacactcc cggagactac tgttgttaga cgtcgaggca ggtcacctag 
      601 aagaagaact ccttcgcctc gcaggcgaag gtctcaatcg ccgcggcgcc gaagatctca 
      661 atctcgggaa tctcaatgtt agtgaagctt ccgggccggc gataatacgc cggcccgttt 
      721 tttttcagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 
      781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 
      841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 
      901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 
      961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 
     1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 
     1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 
     1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 
     1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 
     1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 
     1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 
     1381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 
     1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 
     1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 
     1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 
     1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 
     1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 
     1741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 
     1801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 
     1861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 
     1921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 
     1981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 
     2041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 
     2101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 
     2161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 
     2221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 
     2281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 
     2341 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 
     2401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 
     2461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 
     2521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 
     2581 tcatactctt cctttttcaa tattatgtaa gcagacagtt ttattgttca tgatgatata 
     2641 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata 
     2701 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt 
     2761 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc 
     2821 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca 
     2881 gcaacacctt cttcacgagg cagacctcag cgccggtgat gccggccacg atgcgtccgg 
     2941 cgtagaggat ctctcaccta ccaaacaatg cccccctgca aaaaataaat tcatataaaa 
     3001 aacatacaga taaccatctg cggtgataaa ttatctctgg cggtgttgac ataaatacca 
     3061 ctggcggtga tactgagcac atcagcagga cgcactgacc accatgaagg tgacgctctt 
     3121 aaaattaagc cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg 
     3181 aaacgaagca ttggaata 
//
