LOCUS       pBLCAT5                 4404 bp    DNA     circular     10-FEB-2009
DEFINITION  LMBP 2451.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2451
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium)
            Last modified: 10-FEB-2009
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|509631523|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBLCAT5|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTAUTHOREML|bccm.lmbp@dmbr.UGent.be|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBLCAT5" 0 4404 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 80) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "HindII" "HindIII" "RsaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 844 843 1 4404 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 3 65 0 456 0 1863 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindII" "gtyrac" 4 0 4 22 0 182 0 1707 0 4267 
COMMENT            0 0 "") #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "RsaI" "gtac" 3 0 5 335 0 873 0 1535 0 1853 0 
COMMENT            3592 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "HSV-TK promoter" 29 0 0 39 143 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "39..194") 
COMMENT        #5=(CFSignal (CObList) "part of SV40 small t-antigen" 4 0 0 991 1065 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "991..1065") 
COMMENT        #6=(CFSignal (CObList) "intron of SV40 small t-antigen" 15 0 0 1066 1132 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "1066..1132") 
COMMENT        #7=(CFSignal (CObList) "3' UTR of SV40 small t-antigen" 21 0 0 1133 1684 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "1133..1684") 
COMMENT        #8=(CFSignal (CObList) "SV40 polyA early" 43 0 0 1685 1781 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1685..1781") 
COMMENT        #9=(CFSignal (CObList) "SV40 polyA late" 43 0 1 1685 1781 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(1685..1781)") 
COMMENT        #10=(CFSignal (CObList) "lac promoter" 30 0 1 1920 2003 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1920..2003)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 2095 2497 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2095..2497)") 
COMMENT        #12=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3038 3898 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3038..3898)") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4030 4104 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4030..4104") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4030 4104 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4030..4104)") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4245 4341 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4245..4341") 
COMMENT        #16=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4245 4341 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4245..4341)") 
COMMENT        #17=(CFSignal (CObList) "cat" 4 0 0 242 901 0 (CStringList) 
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COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #18=(CFSignal (CObList) "LMBP 2451" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "Last modified: 10-FEB-2009" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pBLCAT5'" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 2451" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/10/09 12:18" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4404 bp" 1) 
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COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "Modification Date in the Original DB: 10-FEB-2009" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 2451") 
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COMMENT                                        "Start: 242   End: 901 " 1) 
COMMENT                                   #44=(CLinePar 
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COMMENT                                        "chloramphenicol acetyltransferase coding sequence" 
COMMENT                                        1) 
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COMMENT                                 "part of SV40 small t-antigen") 
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COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                 "intron of SV40 small t-antigen"))) 
COMMENT                          "Intron (1 total)") 
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COMMENT                                 "3' UTR of SV40 small t-antigen"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "   39..194" 1))) "HSV-TK promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "   complement(1920..2003)" 1))) 
COMMENT                                 "lac promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                 "pMB1 ori"))) "Replication Origin (1 total)") 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     promoter        39..143
                     /vntifkey="29"
                     /label=HSV-TK\promoter
                     /note="Herpes simplex thymidine kinase promoter"
     CDS             242..901
                     /vntifkey="4"
                     /label=cat
                     /note="chloramphenicol acetyltransferase coding sequence"
     CDS             991..1065
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
     intron          1066..1132
                     /vntifkey="15"
                     /label=intron\of\SV40\small\t-antigen
     misc_feature    1133..1684
                     /vntifkey="21"
                     /label=3'\UTR\of\SV40\small\t-antigen
     terminator      1685..1781
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(1685..1781)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     promoter        complement(1920..2003)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2095..2497)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3038..3898)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     terminator      4030..4104
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4030..4104)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      4245..4341
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(4245..4341)
                     /vntifkey="43"
                     /label=SV40\polyA\late
BASE COUNT     1196 a      1004 c       976 g      1228 t 
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt 
       61 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat 
      121 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt 
      181 caacagcgtg ccgcagatct cgaggagctt ggcgagattt tcaggagcta aggaagctaa 
      241 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga 
      301 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga 
      361 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat 
      421 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg 
      481 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga 
      541 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata 
      601 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga 
      661 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt 
      721 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg 
      781 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca 
      841 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta 
      901 atttttttaa ggcagttatt ggtgccctta aacgcctggt gctacgcctg aataagtgat 
      961 aataagcgga tgaatggcag aaattcgccg gatctttgtg aaggaacctt acttctgtgg 
     1021 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt 
     1081 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac 
     1141 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg 
     1201 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc 
     1261 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt 
     1321 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa 
     1381 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag 
     1441 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt 
     1501 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg 
     1561 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca 
     1621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 
     1681 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 
     1741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     1801 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccccgg gtaccgagct 
     1861 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     1921 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     1981 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     2041 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     2101 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     2161 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     2221 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 
     2281 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 
     2341 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 
     2401 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 
     2461 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 
     2521 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 
     2581 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 
     2641 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 
     2701 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 
     2761 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 
     2821 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 
     2881 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 
     2941 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 
     3001 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 
     3061 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 
     3121 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 
     3181 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 
     3241 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 
     3301 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 
     3361 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 
     3421 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 
     3481 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 
     3541 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 
     3601 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 
     3661 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 
     3721 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 
     3781 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 
     3841 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 
     3901 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 
     3961 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 
     4021 tgccacctga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     4081 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     4141 aatgtatctt atcatgtctg gatcataatc agccatacca catttgtaga ggttttactt 
     4201 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 
     4261 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     4321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     4381 gtatcttatc atgtctggat ctcg 
//
