LOCUS       pBR325OMMBf             7009 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 2146.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     LSOWNER|
COMMENT     VNTNAME|pBR325OMMBf|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBR325OMMBf" 0 7009 
COMMENT       (CDBMol 0 0 1 1 1 0 0 105 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
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COMMENT        #3=(CFSignal (CObList) "TET" 4 0 0 1301 2491 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1301..2491") 
COMMENT        #4=(CFSignal (CObList) "ORI" 33 0 1 3560 3970 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3560..3970)") 
COMMENT        #5=(CFSignal (CObList) "VHf" 4 0 0 4822 4857 0 (CStringList) 
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COMMENT        #6=(CFSignal (CObList) "H1-4" 4 0 0 4858 5043 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4858..5043") 
COMMENT        #7=(CFSignal (CObList) "CH2" 4 0 0 5044 5376 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5044..5376") 
COMMENT        #8=(CFSignal (CObList) "CH3" 4 0 0 5377 5697 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5377..5697") 
COMMENT        #9=(CFSignal (CObList) "3UTR" 21 0 0 5698 5822 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5698..5822") 
COMMENT        #10=(CFSignal (CObList) "CAM" 4 0 0 6794 444 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6794..444")) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA 'pBR325OMMBf'" 1) 
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COMMENT                          "Foreign object. Author: UNKNOWN. Original author: UNKNOWN" 
COMMENT                          1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7009 bp" 1) 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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FEATURES             Location/Qualifiers
     CDS             1301..2491
                     /vntifkey="4"
                     /label=TET
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      complement(3560..3970)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             4822..4857
                     /vntifkey="4"
                     /label=VHf
                     /note="fragment of VH of the human ì3 heavy chain"
     CDS             4858..5043
                     /vntifkey="4"
                     /label=H1-4
                     /note="hinge domain of HIG3"
     CDS             5044..5376
                     /vntifkey="4"
                     /label=CH2
                     /note="constant domain 2 of the human ì3 heavy chain"
     CDS             5377..5697
                     /vntifkey="4"
                     /label=CH3
                     /note="constant domain 3 of the human ì3 heavy chain"
     misc_feature    5698..5822
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of HIG3"
     CDS             6794..444
                     /vntifkey="4"
                     /label=CAM
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1630 a      1961 c      1825 g      1593 t 
ORIGIN
        1 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 
       61 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 
      121 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 
      181 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 
      241 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 
      301 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 
      361 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 
      421 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 
      481 ggtgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt 
      541 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt 
      601 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca 
      661 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc 
      721 ccagagcctg ataaaaacgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc 
      781 gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg 
      841 gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg 
      901 acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc 
      961 tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg 
     1021 atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac 
     1081 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg 
     1141 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg 
     1201 aataccgcaa gcgacaggcc atgtttgaca gcttatcatc gataagcttt aatgcggtag 
     1261 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct 
     1321 catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt 
     1381 actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt 
     1441 gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc 
     1501 cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta 
     1561 cgcgatcatg gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg 
     1621 catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga 
     1681 agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg 
     1741 ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc 
     1801 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg 
     1861 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 
     1921 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 
     1981 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat 
     2041 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac 
     2101 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga 
     2161 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat 
     2221 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca 
     2281 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac 
     2341 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa 
     2401 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg 
     2461 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga 
     2521 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc 
     2581 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc 
     2641 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc 
     2701 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga 
     2761 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc 
     2821 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc 
     2881 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg 
     2941 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt 
     3001 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg 
     3061 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg 
     3121 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc 
     3181 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc 
     3241 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt 
     3301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 
     3361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 
     3421 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg 
     3481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 
     3541 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     3601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     3661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     3721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     3781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     3841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     3901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     3961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     4201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     4561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     4621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     4681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     4741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     4801 tgcgcaacgt tgttgccatt gctgcaggag tcgggcccag gactggggaa gcctccagag 
     4861 ctcaaaaccc cacttggtga cacaactcac acatgcccac ggtgcccaga gcccaaatct 
     4921 tgtgacacac ctcccccgtg cccacggtgc ccagagccca aatcttgtga cacacctccc 
     4981 ccatgcccac ggtgcccaga gcccaaatct tgtgacacac ctcccccgtg cccaaggtgc 
     5041 ccagcacctg aactcttggg aggaccgtca gtcttcctct tccccccaaa acccaaggat 
     5101 acccttatga tttcccggac ccctgaggtc acgtgcgtgg tggtggacgt gagccacgaa 
     5161 gaccccgagg tccagttcaa gtggtacgtg gacggcgtgg aggtgcataa tgccaagaca 
     5221 aagctgcggg aggagcagta caacagcacg ttccgtgtgg tcagcgtcct caccgtcctg 
     5281 caccaggact ggctgaacgg caaggagtac aagtgcaagg tctccaacaa agccctccca 
     5341 gcccccatcg agaaaaccat ctccaaagcc aaaggacagc cccgagaacc acaggtgtac 
     5401 accctgcccc catcccggga ggagatgacc aagaaccaag tcagcctgac ctgcctggtc 
     5461 aaaggcttct accccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac 
     5521 aactacaaca ccacgcctcc catgctggac tccgacggct ccttcttcct ctacagcaag 
     5581 ctcaccgtgg acaagagcag gtggcagcag gggaacatct tctcatgctc cgtgatgcat 
     5641 gaggctctgc acaaccgcta cacgcagaag agcctctccc tgtctccggg taaatgagtg 
     5701 ccatggccgg caagcccccg ctccccgggc tctcggggtc gcgcgaggat gcttggcacg 
     5761 taccccgtgt acatacttcc caggcaccca gcatggaaat aaagcaccca gcgctgccct 
     5821 ggcccccccc ccccccctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     5881 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     5941 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     6001 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     6061 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     6121 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     6181 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     6241 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     6301 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     6361 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     6421 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     6481 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     6541 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcgaat aaatacctgt 
     6601 gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata ccgggaagcc 
     6661 ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt ccaactttca 
     6721 ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat tttcaggagc 
     6781 taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata tatcccaatg 
     6841 gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct ataaccagac 
     6901 cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta 
     6961 tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaa 
//
