LOCUS       pMc254                  3803 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 2098.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 2098
            (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium).
            Last modified: 23-OCT-2007
            
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|460984722|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pMc254|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|LMBP Plasmid Collection|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pMc254" 0 3803 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 90) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "MspA1I" "PdiI" "BsrDI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "2mu ori" 
COMMENT             "AGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTT" 
COMMENT             "Fragment of pAS3; Length: 981" 0 (CStringList) 0) 
COMMENT         #1=(COligo "5' UTR of AOX1" 
COMMENT             "ACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAA" 
COMMENT             "Fragment of pPP1MFmds1; Length: 165" 0 (CStringList) 0) 
COMMENT         #2=(COligo "ADH1 promoter" 
COMMENT             "TGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAGTTTGCCGCTTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAG" 
COMMENT             "Fragment of pAS3; Length: 404" 0 (CStringList) 0) 
COMMENT         #3=(COligo "ADH1 terminator" 
COMMENT             "GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTC" 
COMMENT             "Fragment of pRH007; Length: 102" 0 (CStringList) 0) 
COMMENT         #4=(COligo "AMP promoter onvolledig" 
COMMENT             "CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT" 
COMMENT             "Fragment of pDEST-ECFP; Length: 99 onvolledig" 0 (CStringList) 0) 
COMMENT         #5=(COligo "AMP RBS" "GATAAATGCTTCAATAATATTGAAAAAGGAAGAGT" 
COMMENT             "Fragment of pdECFP; Length: 35" 0 (CStringList) 0) 
COMMENT         #6=(COligo "ampicillin" 
COMMENT             "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" 
COMMENT             "Fragment of pEF6-E-mTRAF2; Length: 861" 0 (CStringList) 0) 
COMMENT         #7=(COligo "AOX1 promoter" 
COMMENT             "AACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGCCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTG" 
COMMENT             "Fragment of pPP1MFmds1; Length: 766" 0 (CStringList) 0) 
COMMENT         #8=(COligo "AOX1 terminator" 
COMMENT             "TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAAGTTCGTTTGTGC" 
COMMENT             "Fragment of pPP1MFmds1; Length: 260" 0 (CStringList) 0) 
COMMENT         #9=(COligo "attR1" 
COMMENT             "ACAAGTTTGTACAAAAAAGCTGAACGAGAAACGTAAAATGATATAAATATCAATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACACAACATATCCAGTCACTATG" 
COMMENT             "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #10=(COligo "attR2" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #11=(COligo "BK ori" "GAGGCCGAGGCCGCCTCTGCCTC" 
COMMENT              "Fragment of pBK-1; Length: 23 " 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #12=(COligo "blasticidin" 
COMMENT              "ATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 399" 0 (CStringList) 0) 
COMMENT         #13=(COligo "bovine growth hormone polyA" 
COMMENT              "CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG" 
COMMENT              "Fragment of pEF1hCASP-9S-His; Length: 225" 0 (CStringList) 0) 
COMMENT         #14=(COligo "CaMV promoter" 
COMMENT              "GATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCT" 
COMMENT              "Fragment of pEX1SOD; Length: 947" 0 (CStringList) 0) 
COMMENT         #15=(COligo "ccdB" 
COMMENT              "ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA" 
COMMENT              "Fragment of pRH001; Length: 306" 0 (CStringList) 0) 
COMMENT         #16=(COligo "CEN6/ARSH4 ori" 
COMMENT              "CTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 525" 0 (CStringList) 0) 
COMMENT         #17=(COligo "chloramphenicol" 
COMMENT              "ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA" 
COMMENT              "Fragment of pRH001; Length: 660" 0 (CStringList) 0) 
COMMENT         #18=(COligo "CYC1 terminator" 
COMMENT              "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT              "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #19=(COligo "CYH2" 
COMMENT              "ATGCCTTCCAGATTCACTAAGACTAGAAAGCACAGAGGTCACGTCTCAGCCGGTAAAGGTCGTATCGGTAAGCACAGAAAGCACCCCGGTGGTAGAGGTATGGCCGGTGGTCAACATCACCACAGAATTAACATGGATAAATACCATCCAGGTTATTTCGGTAAGGTTGGTATGAGATACTTCCACAAGCAACAAGCTCATTTCTGGAAGCCAGTCTTGAACTTGGACAAATTGTGGACATTGATCCCAGAAGACAAGAGAGACCAATACTTGAAATCTGCTTCTAAGGAAACTGCTCCAGTTATTGACACTTTGGCAGCCGGTTACGGTAAGATCTTGGGTAAGGGTAGAATTCCAAATGTTCCAGTTATCGTCAAAGCTAGATTCGTCTCCAAGTTGGCTGAAGAAAAAATCAGAGCTGCTGGTGGTGTTGTTGAATTGATCGCTTAA" 
COMMENT              "Fragment of pAS2; Length: 450" 0 (CStringList) 0) 
COMMENT         #20=(COligo "E-tag" "GGNGCNCCNGTNCCNTAYCCNGAYCCNYTNGARCCNCGN" 
COMMENT              " Fragment of pYD1TSE ; Length: 39" 0 (CStringList) 0) 
COMMENT         #21=(COligo "ECFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pECFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #22=(COligo "EGFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEGFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #23=(COligo "EK recognition site" "GAYGAYGAYGAYAAR" "Length: 15 bp" 0 
COMMENT              (CStringList) 0) 
COMMENT         #24=(COligo "EM7 promoter" 
COMMENT              "GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACC" 
COMMENT              "Fragment of pPICZTS; Length: 66, as in the pEF6 plasmids" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #25=(COligo "EYFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEYFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #26=(COligo "f1 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pAS2; Length: 456" 0 (CStringList) 0) 
COMMENT         #27=(COligo "fd terminator" 
COMMENT              "TAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCC" 
COMMENT              "Fragment of bacteriophage_fd; Length: 82, zoals in pMa58" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #28=(COligo "FLAG-tag" "GAYTAYAARGAYGAYGAYGAYAAR" 
COMMENT              " Fragment of pCAGGS-FLAGmA20; Length: 24" 0 (CStringList) 0) 
COMMENT         #29=(COligo "FLD1 promoter start" 
COMMENT              "GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGATATGCCGCCTATAGGATAAAAACAGGAGA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #30=(COligo "FLD1 promoter stop" 
COMMENT              "TTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTTGATATTCACA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #31=(COligo "FLP terminator" 
COMMENT              "GTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGC" 
COMMENT              "Fragment of pSCGALyEGFP1 compl; Length: 105" 0 (CStringList) 0) 
COMMENT         #32=(COligo "GAL1 promoter" 
COMMENT              "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #33=(COligo "GAL10/CYC1 promoter" 
COMMENT              "ATAACCATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCGTACTTCAATATAGCAATGAGCAGTTAAGCGTATTACTGAAAGTTCCAAAGAGAAGGTTTTTTTAGGCTAATCGACCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACAT" 
COMMENT              "Fragment of pEMBLyex4; Length: 408" 0 (CStringList) 0) 
COMMENT         #34=(COligo "GALad" 
COMMENT              "ATGGATAAAGCGGAATTAATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTACCGCCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG" 
COMMENT              "Fragment of pGAD-mCASP-11; Length: 408" 0 (CStringList) 0) 
COMMENT         #35=(COligo "GALbd" 
COMMENT              "ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCG" 
COMMENT              "Fragment of pAS3; Length: 441" 0 (CStringList) 0) 
COMMENT         #36=(COligo "GALL promoter" 
COMMENT              "TGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of p415GALLmBAX; Length: 369" 0 (CStringList) 0) 
COMMENT         #37=(COligo "GAP promoter" 
COMMENT              "AGATCTTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 483" 0 (CStringList) 0) 
COMMENT         #38=(COligo "GST" 
COMMENT              "ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA" 
COMMENT              "Fragment of pGEXGSTp65(12-317); Length: 654" 0 (CStringList) 0) 
COMMENT         #39=(COligo "GUS start" 
COMMENT              "ATGGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTT" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #40=(COligo "GUS stop" 
COMMENT              "CACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATAA" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #41=(COligo "HA epitope" "TAYCCNTAYGAYGTNCCNGAYTAYGCN" 
COMMENT              "Fragment of pAS2; Length: 27" 0 (CStringList) 0) 
COMMENT         #42=(COligo "hCMV-IE promoter and enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT              "Fragment of pCDM8; Length: 584" 0 (CStringList) 0) 
COMMENT         #43=(COligo "hEF1 promoter start" 
COMMENT              "GCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGG" 
COMMENT              "Fragment of pEF6-MycHisA; Length: 100" 0 (CStringList) 0) 
COMMENT         #44=(COligo "hEF1 promoter stop" 
COMMENT              "ATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGA" 
COMMENT              "Fragment of pEF6-MycHisA; Length: 100" 0 (CStringList) 0) 
COMMENT         #45=(COligo "His-tag" "CAYCAYCAYCAYCAYCAY" "" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #46=(COligo "HIS3 start" 
COMMENT              "ATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGA" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #47=(COligo "HIS3 stop" 
COMMENT              "TAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAG" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #48=(COligo "HSV-TK polyA" 
COMMENT              "AACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCAC" 
COMMENT              "Fragment of pEYFP-N1; Length: 70" 0 (CStringList) 0) 
COMMENT         #49=(COligo "HSV-TK promoter" 
COMMENT              "CCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGA" 
COMMENT              "Fragment of p220.2; Length: 105" 0 (CStringList) 0) 
COMMENT         #50=(COligo "intron AG promoter start" 
COMMENT              "GTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGC" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #51=(COligo "intron AG promoter stop" 
COMMENT              "TCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #52=(COligo "IRES start" 
COMMENT              "CCCCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCC" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #53=(COligo "IRES stop" 
COMMENT              "CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAA" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #54=(COligo "kanamycin" 
COMMENT              "ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT              "Fragment of pRH001; Length: 816" 0 (CStringList) 0) 
COMMENT         #55=(COligo "lac promoter" 
COMMENT              "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT              "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #56=(COligo "lacA" 
COMMENT              "TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAAGGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCATCCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAAAACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGCAATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGATAACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAATTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATCGGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCGGGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGGGTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAATCGTCAGTTTAA" 
COMMENT              "Fragment of pLacDN10HIFNG; Length: 612" 0 (CStringList) 0) 
COMMENT         #57=(COligo "lacI promoter" 
COMMENT              "GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of pREP4; Length: 78" 0 (CStringList) 0) 
COMMENT         #58=(COligo "lacI start" 
COMMENT              "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #59=(COligo "lacI stop" 
COMMENT              "CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #60=(COligo "lacIq promoter" 
COMMENT              "GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of p714; Length: 78" 0 (CStringList) 0) 
COMMENT         #61=(COligo "lacUV5 promoter" 
COMMENT              "CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGG" 
COMMENT              "Fragment of p714; Length: 57" 0 (CStringList) 0) 
COMMENT         #62=(COligo "lacZ RBS" "AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT" 
COMMENT              "Fragment of pGBF-NLScre; Length: 38" 0 (CStringList) 0) 
COMMENT         #63=(COligo "lacZ start" 
COMMENT              "ATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #64=(COligo "lacZ stop" 
COMMENT              "GGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #65=(COligo "LEU2 start" 
COMMENT              "ATGTTTGTTCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTC" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #66=(COligo "LEU2 stop" 
COMMENT              "TTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAA" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #67=(COligo "loxP" "ATAACTTCGTATAGCATACATTATACGAAGTT" 
COMMENT              "Fragment of pCALNL5; Length: 32" 0 (CStringList) 0) 
COMMENT         #68=(COligo "LUC start" 
COMMENT              "ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACG" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #69=(COligo "LUC stop" 
COMMENT              "TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #70=(COligo "M13 ori start" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTT" 
COMMENT              "Fragment of BluescribeM13-; Length: 100" 0 (CStringList) 0) 
COMMENT         #71=(COligo "M13 ori stop" 
COMMENT              "TTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of BluescribeM13-; Length: 100" 0 (CStringList) 0) 
COMMENT         #72=(COligo "mBAX" 
COMMENT              "ATGGACGGGTCCGGGGAGCAGCTTGGGAGCGGCGGGCCCACCAGCTCTGAACAGATCATGAAGACAGGGGCCTTTTTGCTACAGGGTTTCATCCAGGATCGAGCAGGGAGGATGGCTGGGGAGACACCTGAGCTGACCTTGGAGCAGCCGCCCCAGGATGCGTCCACCAAGAAGCTGAGCGAGTGTCTCCGGCGAATTGGAGATGAACTGGATAGCAATATGGAGCTGCAGAGGATGATTGCTGACGTGGACACGGACTCCCCCCGAGAGGTCTTCTTCCGGGTGGCAGCTGACATGTTTGCTGATGGCAACTTCAACTGGGGCCGCGTGGTTGCCCTCTTCTACTTTGCTAGCAAACTGGTGCTCAAGGCCCTGTGCACTAAAGTGCCCGAGCTGATCAGAACCATCATGGGCTGGACACTGGACTTCCTCCGTGAGCGGCTGCTTGTCTGGATCCAAGACCAGGGTGGCTGGGAAGGCCTCCTCTCCTACTTCGGGACCCCCACATGGCAGACAGTGACCATCTTTGTGGCTGGAGTCCTCACCGCCTCGCTCACCATCTGGAAGAAGATGGGCTGA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 579" 0 (CStringList) 0) 
COMMENT         #73=(COligo "mob'" 
COMMENT              "ATGGCGGCATACGCGATCATGCGATGCAAGAAGCTGGCGAAAATGGGCAACGTGGCGGCCAGTCTCAAGCACGCCTACCGCGAGCGCGAGACGCCCAACGCTGACGCCAGCAGGACGCCAGAGAACGAGCACTGGGCGGCCAGCAGCACCGATGAAGCGATGGGCCGACTGCGCGAGTTGCTGCCAGAGAAGCGGCGCAAGGACGCTGTGTTGGCGGTCGAGTACGTCATGACGGCCAGCCCGGAATGGTGGAAGTCGGCCAGCCAAGAACAGCAGGCGGCGTTCTTCGAGAAGGCGCACAAGTGGCTGGCGGACAAGTACGGGGCGGATCGCATCGTGACGGCCAGCATCCACCGTGACGAAACCAGCCCGCACATGACCGCGTTCGTGGTGCCGCTGACGCAGGACGGCAGGCTGTCGGCCAAGGAGTTCATCGGCAACAAAGCGCAGATGACCCGCGACCAGACCACGTTTGCGGCCGCTGTGGCCGATCTAGGGCTGCAACGGGGCATCGAGGGCAGCAAGGCACGTCACACGCGCATTCAGGCGTTCTACGAGGCCCTGGAGCGGCCACCAGTGGGCCACGTCACCATCAGCCCGCAAGCGGTCGAGCCACGCGCCTATGCACCGCAGGGATTGGCCGAAAAGCTGGGAATCTCAAAGCGCGTTGAGACGCCGGAAGCCGTGGCCGACCGGCTGACAAAAGCGGTTCGGCAGGGGTATGAGCCTGCCCTACAGGCCGCCGCAGGAGCGCGTGAGATGCGCAAGAAGGCCGATCAAGCCCAAGAGACGGCCCGAGACCTTCGGGAGCGCCTGAAGCCCGTTCTGGACGCCCTGGGGCCGTTGAATCGGGATATGCAGGCCAAGGCCGCCGCGATCATCAAGGCCGTGGGCGAAAAGCTGCTGACGGAACAGCGGGAAGTCCAGCGCCAGAAACAGGCCCAGCGCCAGCAGGAACGCGGGCGCG" 
COMMENT              "incomplete but functional mobilization gene; 6 C-terminal codons missing" 
COMMENT              0 (CStringList) 0) 
COMMENT         #74=(COligo "Myc epitope" "GARCARAARYTNATHWSNGARGARGAYYTN" 
COMMENT              "Fragment of MYC-TAG; Length: 30" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #75=(COligo "neomycin" 
COMMENT              "ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA" 
COMMENT              "Fragment of pCDNA-mCASP-8; Length: 795" 0 (CStringList) 0) 
COMMENT         #76=(COligo "nos promoter start" 
COMMENT              "GATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGATTGAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #77=(COligo "nos promoter stop" 
COMMENT              "AGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #78=(COligo "ocs terminator start" 
COMMENT              "CTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATC" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #79=(COligo "ocs terminator stop" 
COMMENT              "GTGGGCCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAG" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #80=(COligo "p15A ori start" 
COMMENT              "GGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #81=(COligo "p15A ori stop" 
COMMENT              "GTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #82=(COligo "parA" 
COMMENT              "TTGAAGGTCGCCCGCATCTACCTGCGCGCCAGTACGGACGAGCAGAATCTTGAACGCCAGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTTGCGGAGAAGATCGACCGCATCAGCCGCTTGCCGTTGGCCGAGGCCGAGCGCCTGGTTGCGTCGATCCGGGCCAAAGGGGCCAAGCTGGCCGTGCCTGGCGTGGTGGACCTGTCGGAGCTGGCCGCCGAGGCGAACGGAGTGGCGAAAATCGTTCTGGAATCCGTCCAGGACATGCTTTTGAAGCTCGCCTTGCAGATGGCCCGCGACGACTACGAGGATCGGCGCGAGCGTCAACGTCAGGGTGTCCAGTTGGCGAAGGCCGCCGGCCGCTACACCGGCCGCAAACGTGACGCCGGCATGCACGACCGCATCATCACGCTTCGCTCCGGCGGATCGAGCATTGCCAAGACGGCCAAGCTGGTCGGATGCAGCCCGAGCCAGGTCAAACGAGTGTGGGCGGCCTGGAACGCGCAGCAGCAAAAATAA" 
COMMENT              "Fragment of pRH001; Length: 762" 0 (CStringList) 0) 
COMMENT         #83=(COligo "parB" 
COMMENT              "ATGAAGCGCCGAAGCTACGCCATGCTGCGCGCCGCTGCCGCGCTGGCCGTCCTGGTCGTTGCCTCGCCGGCATGGGCCGAGCTGCGCGGCGAGGTCGTGCGCATCATCGACGGCGACACCATCGACGTGCTGGTAGACAAGCAGCCGGTGCGCGTGCGCCTGGTGGACATTGACGCGCCGGAAAAGCGGCAAGCCTTCGGCGAACGTGCGCGCCAGGCGCTGGCCGGCATGGTGTTCCGCCGGCACGTCCTGGTCGACGAGAAGGACACCGACCGTTACGGCCGCACGCTGGGCACCGTGTGGGTCAACATGGAGCTGGCCAGCCGGCCGCCGCAGCCGCGCAACGTCAACGCCGCGATGGTTCACCAGGGCATGGCGTGGGCCTATCGCTTCCACGGCCGCGCGGCCGACCCTGAAATGCTGCGGCTCGAACAGGAGGCGCGAGGCAAGCGCGTCGGCCTCTGGTCCGATCCGCACGCCGTCGAGCCGTGGAAATGGCGACGCGAGAGCAACAACCGGAGGGACGAAGGTTGA" 
COMMENT              "Fragment of pRH001; Length: 534" 0 (CStringList) 0) 
COMMENT         #84=(COligo "parC" 
COMMENT              "ATGGGTATACCCAATTTGACCAAGGGGGACGCGATGAGGGCGGCCAAGCACTACCGACAACTTCTATCCATCGACTTCAACATCGAGGCGCTGGCCTTCGTGCCTGGACCCGACGGCACACGCGGCCGGCGCATCCACGTCCTGGGGCGCGAGGTCCGCGACCGGCCCGGCCTGGTCGAGTACCTTTCGCCGGCGTTCGGCTCGCGGGTGGCGCTGGACGGCTACTGCAAGGCCAATTTCGATGCAGTGCTGCACCTGGCGTACCCCGATCATCAGCAATGGGGCCACGCATGA" 
COMMENT              "Fragment of pRH001; Length: 294" 0 (CStringList) 0) 
COMMENT         #85=(COligo "parD" 
COMMENT              "ATGAGCCGCCTGACAATCGACATGACGGACCAGCAGCACCAGAGCCTGAAAGCCCTGGCCGCCTTGCAGGGCAAGACCATTAAGCAATACGCCCTCGAACGTCTGTTCCCCGGTGACGCTGATGCCGATCAGGCATGGCAGGAACTGAAAACCATGCTGGGGAACCGCATCAACGATGGGCTTGCCGGCAAGGTGTCCACCAAGAGCGTCGGCGAAATTCTTGATGAAGAACTCAGCGGGGATCGCGCTTGA" 
COMMENT              "Fragment of pMR10cat; Length: 252" 0 (CStringList) 0) 
COMMENT         #86=(COligo "parE" 
COMMENT              "TTGACGGCCTACATCCTCACGGCTGAGGCCGAAGCCGATCTACGCGGCATCATCCGCTACACGCGCCGGGAGTGGGGCGCGGCGCAGGTGCGCCGCTATATCGCTAAGCTGGAACAGGGCATAGCCAGGCTTGCCGCCGGCGAAGGCCCGTTTAAGGACATGAGCGAACTCTTTCCCGCGCTGCGGATGGCCCGCTGCGAACACCACTACGTTTTTTGCCTGCCGCGTGCGGGCGAACCCGCGTTGGTCGTGGCGATCCTGCATGAGCGCATGGACCTCATGACGCGACTTGCCGACAGGCTCAAGGGCTGA" 
COMMENT              "Fragment of pMR10cat; Length: 312" 0 (CStringList) 0) 
COMMENT         #87=(COligo "pBBR1 ori" 
COMMENT              "ATGGCCACGCAGTCCAGAGAAATCGGCATTCAAGCCAAGAACAAGCCCGGTCACTGGGTGCAAACGGAACGCAAAGCGCATGAGGCGTGGGCCGGGCTTATTGCGAGGAAACCCACGGCGGCAATGCTGCTGCATCACCTCGTGGCGCAGATGGGCCACCAGAACGCCGTGGTGGTCAGCCAGAAGACACTTTCCAAGCTCATCGGACGTTCTTTGCGGACGGTCCAATACGCAGTCAAGGACTTGGTGGCCGAGCGCTGGATCTCCGTCGTGAAGCTCAACGGCCCCGGCACCGTGTCGGCCTACGTGGTCAATGACCGCGTGGCGTGGGGCCAGCCCCGCGACCAGTTGCGCCTGTCGGTGTTCAGTGCCGCCGTGGTGGTTGATCACGACGACCAGGACGAATCGCTGTTGGGGCATGGCGACCTGCGCCGCATCCCGACCCTGTATCCGGGCGAGCAGCAACTACCGACCGGCCCCGGCGAGGAGCCGCCCAGCCAGCCCGGCATTCCGGGCATGGAACCAGACCTGCCAGCCTTGACCGAAACGGAGGAATGGGAACGGCGCGGGCAGCAGCGCCTGCCGATGCCCGATGAGCCGTGTTTTCTGGACGATGGCGAGCCGTTGGAGCCGCCGACACGGGTCACGCTGCCGCGCCGGTAG" 
COMMENT              "" 0 (CStringList) 0) 
COMMENT         #88=(COligo "pColE1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCTACGTGGCCTTCTTCCTGTTCCTGGTCTTTTGCTCACATGTTCTTTCCGGCCTTATCCCCTGATTCTGTGGATAACTGTGTTACCGTTTTTGTGTGAGTCAGTACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAAAGC" 
COMMENT              "Fragment of pPLa2311trpA1; Length: 392" 0 (CStringList) 0) 
COMMENT         #89=(COligo "PL promoter" 
COMMENT              "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT              "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0) 
COMMENT         #90=(COligo "pMB1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT              "Fragment of pRH007; Length: 403" 0 (CStringList) 0) 
COMMENT         #91=(COligo "ppMF" 
COMMENT              "ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 243" 0 (CStringList) 0) 
COMMENT         #92=(COligo "R6K ori" 
COMMENT              "TCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTCAGTGACACAGGAACACTTAACGGCTGACAT" 
COMMENT              "Fragment of pKNG101; Length: 388" 0 (CStringList) 0) 
COMMENT         #93=(COligo "RK2 ori" 
COMMENT              "GGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGG" 
COMMENT              "Fragment of pRH001; Length: 526" 0 (CStringList) 0) 
COMMENT         #94=(COligo "RK2/RP4 oriT" 
COMMENT              "CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG" 
COMMENT              "Fragment of pRH001; Length: 111" 0 (CStringList) 0) 
COMMENT         #95=(COligo "rop" 
COMMENT              "GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA" 
COMMENT              "Fragment of pBR322; Length: 192" 0 (CStringList) 0) 
COMMENT         #96=(COligo "SP6 promoter" "ATTTAGGTGACACTATA" 
COMMENT              "Fragment of pdCS2-MT ; Length: 17" 0 (CStringList) 0) 
COMMENT         #97=(COligo "Strep-tag" "GCTTGGCGTCACCCGCAGTTCGGTGGT" 
COMMENT              "pUChGNTI2: 27 nucleotides" 0 (CStringList) 0) 
COMMENT         #98=(COligo "SV40 early promoter" 
COMMENT              "CTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCC" 
COMMENT              "Fragment of pEF6/Myc-HisA; Length: 282" 0 (CStringList) 0) 
COMMENT         #99=(COligo "SV40 ori" 
COMMENT              "GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCKGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT" 
COMMENT              "Fragment of SV40; Length: 76" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #100=(COligo "SV40 polyA early" 
COMMENT               "AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTT" 
COMMENT               "Fragment of pdEYFP; Length: 97" 0 (CStringList) 0) 
COMMENT         #101=(COligo "T1 terminator" 
COMMENT               "ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT" 
COMMENT               "Fragment of pDONR201; Length: 44" 0 (CStringList) 0) 
COMMENT         #102=(COligo "T2 terminator" "AGAAGGCCATCCTGACGGATGGCCTTTT" 
COMMENT               "Fragment of pDONR201; Length: 28" 0 (CStringList) 0) 
COMMENT         #103=(COligo "T3 promoter" "AATTAACCCTCACTAAA" 
COMMENT               "Fragment of pLT-mCASP-14" 0 (CStringList) 0) 
COMMENT         #104=(COligo "T7 promoter" "TAATACGACTCACTATA" 
COMMENT               "Fragment of pGEM-TAX1BP1-S; Length: 17" 0 (CStringList) 0) 
COMMENT         #105=(COligo "T7 RBS" 
COMMENT               "GGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT" 
COMMENT               "Fragment of pLT10Bgal; Length: 63" 0 (CStringList) 0) 
COMMENT         #106=(COligo "T7 terminator" 
COMMENT               "CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG" 
COMMENT               "Fragment of pGBKT7; Length: 48" 0 (CStringList) 0) 
COMMENT         #107=(COligo "tac promoter" "GTTGACAATTAATCATCGGCTCGTATAATGTGTGG" 
COMMENT               "Fragment of pGEX5X1; Length: 35" 0 (CStringList) 0) 
COMMENT         #108=(COligo "TEF1 E. gossypii" 
COMMENT               "GGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTC" 
COMMENT               "E. gossypii TEF1alpha promoter" 0 (CStringList) 0) 
COMMENT         #109=(COligo "TEF1 terminator start" 
COMMENT               "AGTAACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATT" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #110=(COligo "TEF1 terminator stop" 
COMMENT               "ATATTTTTTTTGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #111=(COligo "TEF1a promoter start" 
COMMENT               "CACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATA" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #112=(COligo "TEF1a promoter stop" 
COMMENT               "GGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGYTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB" "nagekeken 06/2005 AO") 0) 
COMMENT         #113=(COligo "TET start" 
COMMENT               "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGG" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #114=(COligo "TET stop" 
COMMENT               "CACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #115=(COligo "thrombin recognition site" "CTGGTTCCGCGTGGATCC" 
COMMENT               "Fragment of pGEX-4T2; Length: 18" 0 (CStringList) 0) 
COMMENT         #116=(COligo "TRP1" 
COMMENT               "ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG" 
COMMENT               "Fragment of pAS2; Length: 675" 0 (CStringList) 0) 
COMMENT         #117=(COligo "URA3" 
COMMENT               "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT               "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0) 
COMMENT         #118=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT               "length: 42 bp" 0 (CStringList) 0) 
COMMENT         #119=(COligo "VSV epitope" "TACACCGACATCGAGATGAACCGGTTGGGCAAG" 
COMMENT               "Fragment of pCR3VSV-CED-4; Length: 33" 0 (CStringList) 0) 
COMMENT         #120=(COligo "XGPRT" 
COMMENT               "ATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAAGTGCTGAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAA" 
COMMENT               "Fragment of pDelta1; Length: 459" 0 (CStringList) 0) 
COMMENT         #121=(COligo "Xpress epitope" "GAYYTNTAYGAYGAYGAYGAYAAR" 
COMMENT               "Fragment of pYD1TSE ; Length: 24" 0 (CStringList) 0) 
COMMENT         #122=(COligo "zeocin" 
COMMENT               "ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 375" 0 (CStringList) 0) 
COMMENT         #123=(COligo "\337-globin polyA" 
COMMENT               "GATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGSTAATAAAGGAAATTTATTTTCATTGC" 
COMMENT               "Fragment of pBI-G; Length: 92" 0 (CStringList) 0)) 1 "{(0,1),2}" 
COMMENT        0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 3803 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #124=(CRSite (CStringList) "MspA1I" "cmgckg" 4 0 5 1267 0 1512 0 2453 0 
COMMENT              2963 0 3407 0 0 "") 
COMMENT        #125=(CRSite (CStringList) "PdiI" "gccggc" 4 0 1 699 0 0 "") 
COMMENT        #126=(CRSite (CStringList) "BsrDI" "gcaatg" 9 7 4 1879 0 2053 1 2681 0 
COMMENT              3290 1 0 "")) 
COMMENT       (CObList 
COMMENT        #127=(CFSignal (CObList) "fd terminator" 43 0 0 24 105 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "24..105") 
COMMENT        #128=(CFSignal (CObList) "fd terminator" 43 0 0 134 215 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "134..215") 
COMMENT        #129=(CFSignal (CObList) "f1 ori" 33 0 1 371 826 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(371..826)") 
COMMENT        #130=(CFSignal (CObList) "pMB1 ori" 33 0 1 830 1203 0 (CStringList) 
COMMENT              (CStringList) 1 1 1 1 "complement(830..1203)") 
COMMENT        #131=(CFSignal (CObList) "ampicillin sensitive" 4 0 1 1744 2604 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "complement(1744..2604)") 
COMMENT        #132=(CFSignal (CObList) "chloramphenicol" 4 0 1 2861 3520 0 
COMMENT              (CStringList) (CStringList) 1 1 1 1 "complement(2861..3520)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #133=(CFSignal (CObList) "LMBP 2098" 2 0 0 0 0 0 (CStringList) 
COMMENT              (CStringList) 1 1 0 0 "") 
COMMENT        #134=(CFSignal (CObList) "Last modified: 23-OCT-2007" 2 0 0 0 0 0 
COMMENT              (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #135=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT              (CObjectList 
COMMENT               #136=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pMc254" 2) 5 "" 
COMMENT                     0 4) 
COMMENT               #137=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #138=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "DNA Plasmid 'pMc254'" 1) 
COMMENT                       #139=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "LMBP 2098." 1) 
COMMENT                       #140=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Currently local object. Original author: LMBP Plasmid Collection" 
COMMENT                             1) 
COMMENT                       #141=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Creation Date: ? (no data)" 1) 
COMMENT                       #142=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Last Modified: 10/23/07 11:18" 1) 
COMMENT                       #143=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "length: 3803 bp" 1) 
COMMENT                       #144=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "storage type: Basic" 1) 
COMMENT                       #145=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "form: Circular" 1))) "General Description") 
COMMENT               #146=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #147=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Original Source Database: GenBank" 1) 
COMMENT                       #148=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "Modification Date in the Original DB: 1-AUG-2007" 
COMMENT                             1))) "Standard Fields") 
COMMENT               #149=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 4 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #150=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "LMBP Plasmid Collection" 1))) "Author") 
COMMENT               #151=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 5 (0 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #152=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                             "LMBP Plasmid Collection" 1))) "Original Author") 
COMMENT               #153=(CRefLinePar 
COMMENT                     (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 
COMMENT                     "" 0 0) 
COMMENT               #154=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #155=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                              (CObjectList 
COMMENT                               #156=(CLinePar 
COMMENT                                     (CParagraph 0 (15 #133# 0) 1 2 2 0 180) "" 
COMMENT                                     1))) "LMBP 2098") 
COMMENT                       #157=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                              (CObjectList 
COMMENT                               #158=(CLinePar 
COMMENT                                     (CParagraph 0 (15 #134# 0) 1 2 2 0 180) "" 
COMMENT                                     1))) "Last modified: 23-OCT-2007"))) 
COMMENT                     "Annotations") 
COMMENT               #159=(CFolderPar 
COMMENT                     (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                      (CObjectList 
COMMENT                       #160=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #161=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 1744 (3 #131# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #162=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 1744  End: 2604 (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #163=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "ampicillin sensitive; mutated coding sequence" 
COMMENT                                             1) 
COMMENT                                       #164=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #165=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(1744..2604)" 1))) 
COMMENT                                     "ampicillin sensitive") 
COMMENT                               #166=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 2861 (3 #132# 0) 1 2 2 0 327) 
COMMENT                                      (CObjectList 
COMMENT                                       #167=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 2861  End: 3520 (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #168=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "chloramphenicol resistance gene; coding sequence" 
COMMENT                                             1) 
COMMENT                                       #169=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #170=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(2861..3520)" 1))) 
COMMENT                                     "chloramphenicol"))) "CDS (2 total)") 
COMMENT                       #171=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #172=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 371 (3 #129# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #173=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 371   End: 826  (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #174=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "phage f1 origin" 1) 
COMMENT                                       #175=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #176=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(371..826)" 1))) 
COMMENT                                     "f1 ori") 
COMMENT                               #177=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 830 (3 #130# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
COMMENT                                       #178=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Start: 830   End: 1203 (Complementary)" 
COMMENT                                             1) 
COMMENT                                       #179=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "origin plasmid" 1) 
COMMENT                                       #180=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "Original Location Description:" 1) 
COMMENT                                       #181=(CLinePar 
COMMENT                                             (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                             "   complement(830..1203)" 1))) 
COMMENT                                     "pMB1 ori"))) 
COMMENT                             "Replication Origin (2 total)") 
COMMENT                       #182=(CFolderPar 
COMMENT                             (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #183=(CFolderPar 
COMMENT                                     (CGroupPar 
COMMENT                                      (CParagraph 24 (3 #127# 0) 1 2 2 0 194) 
COMMENT                                      (CObjectList 
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FEATURES             Location/Qualifiers
     terminator      24..105
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="phage fd terminator"
     terminator      134..215
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="phage fd terminator"
     rep_origin      complement(371..826)
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     rep_origin      complement(830..1203)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(1744..2604)
                     /vntifkey="4"
                     /label=ampicillin\sensitive
                     /note="ampicillin sensitive; mutated coding sequence"
     CDS             complement(2861..3520)
                     /vntifkey="4"
                     /label=chloramphenicol
                     /note="chloramphenicol resistance gene; coding sequence"
BASE COUNT     1030 a       907 c       885 g       981 t 
ORIGIN
        1 aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt 
       61 ttttttggag attttcaacg tgaaaaaatt attattcgca attccaagct aattcacctc 
      121 gaaagcaagc tgataaaccg atacaattaa aggctccttt tggagccttt ttttttggag 
      181 attttcaacg tgaaaaaatt attattcgca attccaagct ctgcctcgcg cgtttcggtg 
      241 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
      301 cggatgcaga tcaccccggt tgataatcag aaaagcccca aaaacaggaa gattgtataa 
      361 gcaaatattt aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 
      421 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa 
      481 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 
      541 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 
      601 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct 
      661 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 
      721 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 
      781 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtgatc tgcgctcggt 
      841 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
      901 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
      961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     1021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     1081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     1141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     1201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     1261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     1321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     1381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     1441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     1501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     1561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     1621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     1681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     1741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     1801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     1861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     1921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     1981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     2041 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     2101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     2161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     2221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     2281 ttctgtgact ggtgactact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     2341 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 
     2401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     2461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     2521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     2581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcagacagtt 
     2641 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca 
     2701 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gactccccgc gcgcgatggg 
     2761 tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt caggcgtagc 
     2821 aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg ccctgccact 
     2881 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacagacgg 
     2941 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc 
     3001 ccatagtgaa aacgggggcg aagaagttgt ccatattcgc cacgtttaaa tcaaaactgg 
     3061 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga 
     3121 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc 
     3181 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa 
     3241 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac 
     3301 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact 
     3361 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt 
     3421 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg 
     3481 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg 
     3541 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt 
     3601 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc 
     3661 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta 
     3721 tttattcgaa gacgaaaggg catcgcgcgc ggggaattcc cggggatccg tcgacctgca 
     3781 gccaagcttg gtctagaggt cga 
//
