ID pLacDN6FHIFNG standard; circular DNA; ; 9484 BP. DT 4-AUG-2009 DE LMBP 2011. CC Accession number: LMBP 2011 CC (c) BCCM/LMBP Plasmid collection (Ghent University, Belgium) CC Last modified: 4-AUG-2009 CC CC VNTAUTHORNAME|LMBP Plasmid Collection| CC VNTAUTHOREML|bccm.lmbp@irc.UGent.be| CC VNTREPLTYPE|Plasmid CC FH Key Location/Qualifiers FH FT CDS 15..3062 FT /vntifkey="4" FT /label=lacZ\fragment FT CDS 3114..4367 FT /vntifkey="4" FT /label=lacY FT CDS 4431..4978 FT /vntifkey="4" FT /label=lacA\fragment FT terminator 5600..5681 FT /vntifkey="43" FT /label=fd\terminator FT terminator 5710..5791 FT /vntifkey="43" FT /label=fd\terminator FT rep_origin 5889..6344 FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(6348..6721) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(7262..8122) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter 8488..8618 FT /vntifkey="30" FT /label=PL\promoter FT promoter 8812..8907 FT /vntifkey="30" FT /label=amp\promoter FT CDS 9055..14 FT /vntifkey="4" FT /label=hIFNG\mature\sequence FT RBS 8908..8942 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT RBS 9026..9054 FT /vntifkey="32" FT /label=5'\UTR\of\trp\operon,\incl.\RBS FT CDS 8943..9011 FT /vntifkey="4" FT /label=amp\signal\sequence SQ Sequence 9484 BP; 2312 A; 2366 C; 2369 G; 2431 t; 6 other; ttcccgggga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 60 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 120 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc 180 cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg 240 tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtaa 300 cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact 360 cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg 420 atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt tacggccagg 480 acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga gaaaaccgcc 540 tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag gatatgtggc 600 ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca caaatcagcg 660 atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg gaggctgaag 720 ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta tggcagggtg 780 aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat gagcgtggtg 840 gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg 900 aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc acgctgattg 960 aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc 1020 tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat cctctgcatg 1080 gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag cagaacaact 1140 ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg ctgtgcgacc 1200 gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg gtgccaatga 1260 atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta acgcgaatgg 1320 tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat gaatcaggcc 1380 acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg 1440 tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc ccgatgtacg 1500 cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc 1560 tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac gcgatgggta 1620 acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc cgtttacagg 1680 gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa aacggcaacc 1740 cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag ttctgtatga 1800 acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca aaacaccagc 1860 agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc gaatacctgt 1920 tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt aagccgctgg 1980 caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt gaactgcctg 2040 aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta gtgcaaccga 2100 acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg 2160 aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg accaccagcg 2220 aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc cagtcaggct 2280 ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg cgcgatcagt 2340 tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc attgacccta 2400 acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca gcgttgttgc 2460 agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac gcgtggcagc 2520 atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt agtggtcaaa 2580 tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg cggattggcc 2640 tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta gggccgcaag 2700 aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg ccattgtcag 2760 acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat 2820 tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc cgctacagtc 2880 aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa ggcacatggc 2940 tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc ccgtcagtat 3000 cggcgnnnnn ncagctgagc gccggtcgct accattacca gttggtctgg tgtcaaaaat 3060 aataataacc gggcaggcca tgtctgcccg tatttcgcgt aaggaaatcc attatgtact 3120 atttaaaaaa cacaaacttt tggatgttcg gtttattctt tttcttttac ttttttatca 3180 tgggagccta cttcccgttt ttcccgattt ggctacatga catcaaccat atcagcaaaa 3240 gtgatacggg tattattttt gccgctattt ctctgttctc gctattattc caaccgctgt 3300 ttggtctgct ttctgacaaa ctcgggctgc gcaaatacct gctgtggatt attaccggca 3360 tgttagtgat gtttgcgccg ttctttattt ttatcttcgg gccactgtta caatacaaca 3420 ttttagtagg atcgattgtt ggtggtattt atctaggctt ttgttttaac gccggtgcgc 3480 cagcagtaga ggcatttatt gagaaagtca gccgtcgcag taatttcgaa tttggtcgcg 3540 cgcggatgtt tggctgtgtt ggctgggcgc tgtgtgcctc gattgtcggc atcatgttca 3600 ccatcaataa tcagtttgtt ttctggctgg gctctggctg tgcactcatc ctcgccgttt 3660 tactcttttt cgccaaaacg gatgcgccct cttctgccac ggttgccaat gcggtaggtg 3720 ccaaccattc ggcatttagc cttaagctgg cactggaact gttcagacag ccaaaactgt 3780 ggtttttgtc actgtatgtt attggcgttt cctgcaccta cgatgttttt gaccaacagt 3840 ttgctaattt ctttacttcg ttctttgcta ccggtgaaca gggtacgcgg gtatttggct 3900 acgtaacgac aatgggcgaa ttacttaacg cctcgattat gttctttgcg ccactgatca 3960 ttaatcgcat cggtgggaaa aacgccctgc tgctggctgg cactattatg tctgtacgta 4020 ttattggctc atcgttcgcc acctcagcgc tggaagtggt tattctgaaa acgctgcata 4080 tgtttgaagt accgttcctg ctggtgggct gctttaaata tattaccagc cagtttgaag 4140 tgcgtttttc agcgacgatt tatctggtct gtttctgctt ctttaagcaa ctggcgatga 4200 tttttatgtc tgtactggcg ggcaatatgt atgaaagcat cggtttccag ggcgcttatc 4260 tggtgctggg tctggtggcg ctgggcttca ccttaatttc cgtgttcacg cttagcggcc 4320 ccggcccgct ttccctgctg cgtcgtcagg tgaatgaagt cgcttaagca atcaatgtcg 4380 gatgcggcgc gacgcttatc cgaccaacat atcataacgg agtgatcgca ttgaacatgc 4440 caatgaccga aagaataaga gcaggcaagc tatttaccga tatgtgcgaa ggcttaccgg 4500 aaaaaagact tcgtgggaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaag 4560 ttgaaaaaag agaaagcctg attaaagaaa tgtttgccac ggtaggggaa aacgcctggg 4620 tagaaccgcc tgtctatttc tcttacggtt ccaacatcca tataggccgc aatttttatg 4680 caaatttcaa tttaaccatt gtcgatgact acacggtaac aatcggtgat aacgtactga 4740 ttgcacccaa cgttactctt tccgttacgg gacaccctgt acaccatgaa ttgagaaaaa 4800 acggcgagat gtactctttt ccgataacga ttggcaataa cgtctggatc ggaagtcatg 4860 tggttattaa tccaggcgtc accatcgggg ataattctgt tattggcgcg ggtagtatcg 4920 tcacaaaaga cattccacca aacgtcgtgg cggctggcgt tccttgtcgg gttattcgcc 4980 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 5040 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 5100 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 5160 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 5220 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 5280 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 5340 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 5400 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 5460 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 5520 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcctaga ggtcgaaatt 5580 cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt 5640 ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctaatt cacctcgaaa 5700 gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt ttggagattt 5760 tcaacgtgaa aaaattatta ttcgcaattc caagctctgc ctcgcgcgtt tcggtgatga 5820 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 5880 tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 5940 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 6000 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 6060 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 6120 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 6180 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 6240 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 6300 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttgatctg cgctcggtcg 6360 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 6420 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 6480 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 6540 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 6600 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 6660 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 6720 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 6780 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 6840 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6900 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 6960 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7020 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7080 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 7140 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 7200 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 7260 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 7320 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 7380 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 7440 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 7500 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 7560 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 7620 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 7680 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 7740 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 7800 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 7860 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 7920 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 7980 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8040 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8100 cacggaaatg ttgaatactc atactcttcc tttttcaata ttatgtaagc agacagtttt 8160 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca 8220 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt 8280 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc 8340 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt 8400 caggcctggt atgagtcagc aacaccttct tcacgaggca gacctcagcg ccggtgatgc 8460 cggccacgat gcgtccggcg tagaggatct ctcacctacc aaacaatgcc cccctgcaaa 8520 aaataaattc atataaaaaa catacagata accatctgcg gtgataaatt atctctggcg 8580 gtgttgacat aaataccact ggcggtgata ctgagcacat cagcaggacg cactgaccac 8640 catgaaggtg acgctcttaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc 8700 tttggggtgt gtgatacgaa acgaagcatt ggaattcttg aagacgaaag ggcctcgtga 8760 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca 8820 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 8880 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 8940 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 9000 ctgtttttgc tcaccgccta gtacgcaagt tcacgtaaaa agggtatcga ttccatgcag 9060 gacccatatg taaaagaagc agaaaacctt aagaaatatt ttaatgcagg tcattcagat 9120 gtagcggata atggaactct tttcttaggc attttgaaga attggaaaga ggagagtgac 9180 agaaaaataa tgcagagcca aattgtctcc ttttacttca aactttttaa aaactttaaa 9240 gatgaccaga gcatccaaaa gagtgtggag accatcaagg aagacatgaa tgtcaagttt 9300 ttcaatagca acaaaaagaa acgagatgac ttcgaaaagc tgactaatta ttcggtaact 9360 gacttgaatg tccaacgcaa agcaatacat gaactcatcc aagtgatggc tgaactgtcg 9420 ccagcagcta aaacagggaa gcgaaagcgc agtcagatgc tgtttcgagg tcgaccctct 9480 agaa 9484 //