LOCUS       pPLc321HIFNGHS          5464 bp    DNA     circular     25-FEB-2011
DEFINITION  LMBP 1444.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|582807995|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pPLc321HIFNGHS|
COMMENT     VNTAUTHORNAME|LMBP Plasmid collection|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "475..1074") 
COMMENT        #2=(CFSignal (CObList) "pMB1 ori" 33 0 1 3047 3449 0 (CStringList) 
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COMMENT                        "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                        "Creation Date: ? (no data)" 1) 
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FEATURES             Location/Qualifiers
     CDS             40..474
                     /vntifkey="4"
                     /label=hIFNG
                     /note="mutated human interferon ì mature sequence"
     misc_feature    475..1074
                     /vntifkey="21"
                     /label=3'\\UTR\of\hIFNG
     rep_origin      complement(3047..3449)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3990..4850)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        5216..5346
                     /vntifkey="30"
                     /label=PL
                     /note="phage ñ major leftward promoter"
     misc_feature    5347..39
                     /vntifkey="21"
                     /label=5'\UTR\of\trp\operon,\incl.\RBS
     misc_feature    2612..2803
                     /vntifkey="21"
                     /label=rop
BASE COUNT     1386 a      1378 c      1345 g      1336 t        19 others
ORIGIN
        1 aactagtacg caagttcacg taaaaagggt atcgattcca tgcaggaccc atatgtaaaa 
       61 gaagcagaaa accttaagaa atattttaat gcaggtcatt cagatgtagc ggataatgga 
      121 actcttttct taggcatttt gaagaattgg aaagaggaga gtgacagaaa aataatgcag 
      181 agccaaattg tctcctttta cttcaaactt tttaaaaact ttaaagatga ccagagcatc 
      241 caaaagagtg tggagaccat caaggaagac atgaatgtca agtttttcaa tagcaacaaa 
      301 aagaaacgag atgacttcga aaagctgact aattattcgg taactgactt gaatgtccaa 
      361 cgcaaagcaa tacatgaact catccaagtg atggctgaac tgtcgccagc agctaaaaca 
      421 gggaagcgaa agcgcagtca gatgctgttt cgaggtcgac gagcatccca gtaatggttg 
      481 tcctgcctgc aatatttgaa ttttaaatct aaatctattt attaatattt aacattattt 
      541 atatggggaa tatattttta gactcatcaa tcaaataagt atttataata gcaacttttg 
      601 tgtaatgaaa atgaatatct attaatatat gtattattta taattcctat atcctgtgac 
      661 tgtctcactt aatcctttgt tttctgacta attaggcaag gctatgtgat tacaaggctt 
      721 tatctcaggg gccaactagg cagccaacct aagcaagatc ccatgggttg tgtgtttatt 
      781 tcacttgatg atacaatgaa cacttataag tgaagtgata ctatccagtt actgccggtt 
      841 tgaaaatatg cctgcaatct gagccagtgc tttaatggca tgtcagacag aacttgaatg 
      901 tgtcaggtga ccctgatgaa aacatagcat ctcaggagat ttcatgcctg gtgcttccaa 
      961 atattgttga caactgtgac tgtacccaaa tggaaagtaa ctcatttgtt aaaattatca 
     1021 atatctaata tatatgaata aagtgtaagt tcnnnnnnnn nnnnnnnnnn nggatcctct 
     1081 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 
     1141 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 
     1201 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 
     1261 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 
     1321 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag 
     1381 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 
     1441 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 
     1501 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 
     1561 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 
     1621 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 
     1681 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 
     1741 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 
     1801 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 
     1861 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 
     1921 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 
     1981 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 
     2041 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 
     2101 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat 
     2161 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa 
     2221 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg 
     2281 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 
     2341 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 
     2401 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc 
     2461 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc 
     2521 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat 
     2581 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa 
     2641 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 
     2701 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 
     2761 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
     2821 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
     2881 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 
     2941 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg 
     3001 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc 
     3061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 
     3121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 
     3181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 
     3241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 
     3301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 
     3361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 
     3421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 
     3481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 
     3541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 
     3601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 
     3661 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 
     3721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 
     3781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 
     3841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 
     3901 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 
     3961 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 
     4021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 
     4081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 
     4141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 
     4201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 
     4261 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg 
     4321 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 
     4381 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 
     4441 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 
     4501 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 
     4561 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc 
     4621 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 
     4681 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 
     4741 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 
     4801 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 
     4861 tttcaatatt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc 
     4921 aatgtaacat cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc 
     4981 tgagttgaag gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca 
     5041 aaagttcaaa atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct 
     5101 cactttctgg ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc 
     5161 acgaggcaga cctcagcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctct 
     5221 cacctaccaa acaatgcccc cctgcaaaaa ataaattcat ataaaaaaca tacagataac 
     5281 catctgcggt gataaattat ctctggcggt gttgacataa ataccactgg cggtgatact 
     5341 gagcacatca gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa ttaagccctg 
     5401 aagaagggca gcattcaaag cagaaggctt tggggtgtgt gatacgaaac gaagcattgg 
     5461 aatt 
//
